ABSTRACT
Antiviral agents based on natural products have attracted substantial attention in clinical applications for their distinct biological activities,molecular structuralmultiformities, and low biotoxicities. Ferulic acid (FA) with apigenin propaneto form an esterified FA derivative (FAAP).Herein, we designed a CsPbBr3-modified chitosan oligosaccharide, a biomimetic nanoplatform that could load with FAAP. After self-assembly by combining FAAP with CsPbBr3-modified chitosan oligosaccharide (FAAP NPs), the resulting nanoparticles (FAAP NPs) showed high antioxidant and anti-inflammatory activities for enhancing the inhibition of porcineparvovirus.FAAP NPs exhibited no signs of acute toxicity in vitro or in vivo. DPPH and ABST are widely used for quantitative determination of antioxidant capacity. FAAP NPs exhibited excellent DPPH and ABTS radical scavenging abilities. In addition, we found that FAAP NPs inhibited PPV infection-induced PK-15 cell apoptosis, which was associated with regulating antioxidant and anti-inflammatory signaling pathways. Importantly, we showed that FAAP NPs blocked PPV infection-induced mitochondrial apoptosis in PK-15 cells via a p53/BH3 domain molecular-dependent mechanism.
Subject(s)
Antiviral Agents/pharmacology , Nanoparticles/chemistry , Parvoviridae Infections/veterinary , Parvovirus, Porcine/drug effects , Animals , Antiviral Agents/chemical synthesis , Apigenin/chemistry , Calcium Compounds/chemistry , Cell Line , Chitosan/chemistry , Coumaric Acids/chemistry , Inhibitory Concentration 50 , Oxides/chemistry , Particle Size , Parvoviridae Infections/drug therapy , Parvoviridae Infections/virology , Sus scrofa , Titanium/chemistryABSTRACT
OBJECTIVE: To optimize the sulfated modification conditions of astragalus polysaccharide (APS) and probe into the probability of sulfated modification to enhance APS activity. METHODS: Total APS extracted by one-step ethanol precipitation and three fractional APSs extracted by stepwise ethanol precipitation method and purified were modified by chlorosulfonic acid-pyridine method. The modification conditions were optimized by L9 (3(4)) orthogonal design taking the ratio of chlorosulfonic acid to pyridine, reaction temperature and time. The degree of substitution (DS) was tested by sulfate barium turbidimetric method. The anti-IBDV activity of modification production was tested by MTT method. RESULTS: The optimized modification conditions were 1 : 6 of chlorosulfonic acid to pyridine, reaction temperature of 95 degrees C and reaction time of 1 hour. CONCLUSION: Sulfated modification can enhance the antiviral activity of APS, which related to DS.
