High incidence of coding gene mutations in mitochondrial DNA in esophageal cancer.

The aim of the present study was to detect mutations in the coding genes of mitochondrial DNA (mtDNA) in three esophageal cancer cell lines and in tumor tissues obtained from 30 patients with esophageal cancer, to investigate the relationship between protein­ and RNA­coding gene mutations and esophageal cancer. mtDNA was extracted and the coding genes were sequenced and analyzed by comparing the sequencing results with the complete mitochondrial genome of Homo sapiens. The results revealed 39 mutations in the three esophageal cancer cell lines; the genes with the highest mutation frequencies included mitochondrially encoded cytochrome B (MT­CYTB), NADH dehydrogenase 5 (MT­ND5) and MT­ND4 gene. A total of 216 mutations were identified in the 30 esophageal cancer tissues, including 182 protein­coding mutations, of which MT­CYTB and MT­ND5 genes exhibited higher mutation frequencies. The results of the present study indicated that mutations in the coding genes of mtDNA in esophageal cancer cells may be related to the occurrence of esophageal cancer.

[Spectra of in situ reduction of Ag( I ) by phycocyanin and dynamic formation of Ag(0)].

The interaction of Ag( I ) and Phycocyanin(PC for short) was investigated by ultraviolet-visible, fluorescence and infrared spectra under the condition of 4 degrees C and without light. The results showed that the characteristic absorption of PC at 615 nm decreased gradually with the increase in Ag( I ) concentration and interaction time. Accordingly, its fluorescence emission peak and two fluorescence excitation peaks also decreased. Dynamic formation of nano-Ag(0) was also observed by synchronous fluorescence spectra, showing that Ag(0) was formed by the PC in situ reduction. It was observed by TEM that Ag(0) dispersed in PC, which formed a bioconjugate with PC as the core and nano-Ag(0) as the crust. The bioconjugate was round and its diameter was about 15-30 nm.