ABSTRACT
Polymeric {Cu6[(µ3-η2:η2:η2)2-C60](FPz)6Cl·3C6H5Cl}∞ [FPz = 4-(trifluoromethyl)pyrazolate], synthesized solvothermally with chlorobenzene as the solvent, is a doubly-connecting trans bis-adduct hexanuclear cuprofullerene that has copper in mixed valence. The compound is an example of a metallofullerene having semiconductivity character.
ABSTRACT
The controlled synthesis of high-nuclear regioisomeric core-shell exohedral metallofullerenes (ExMFs) is challenging. Herein, we demonstrated the synthesis of regioisomeric core-shell cuprofullerene C60@CuI24 and its 3-D coordination polymer using heteroleptic ligands, realizing high-nuclear regioisomeric ExMFs and a polymeric ExMF structure.
ABSTRACT
In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the microscope. And then the dermatophytes were cultured on Sabouraud's dextrose agar (SDA). The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction (PCR), and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN software were used to analyze the sequences. To further determine highly homologous sequences, a phylogenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosum and Microsporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate C115LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to microsporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR (JN650537, Cladosporium) and Ascomycete sp. LB68A1A2 (AY770409, Ascomycete sp).
Subject(s)
Humans , Arthrodermataceae , Cell Biology , Genetics , Hyphae , Cell Biology , RNA, Fungal , Genetics , RNA, Ribosomal, 18S , Genetics , Skin , MicrobiologyABSTRACT
In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the microscope. And then the dermatophytes were cultured on Sabouraud's dextrose agar (SDA). The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction (PCR), and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN software were used to analyze the sequences. To further determine highly homologous sequences, a phylogenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosum and Microsporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate C115LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to microsporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR (JN650537, Cladosporium) and Ascomycete sp. LB68A1A2 (AY770409, Ascomycete sp).
ABSTRACT
<p><b>OBJECTIVE</b>To detect bcl-2 gene expression in Epstein-Barr virus (EBV)-infected human gastric epithelial cell line GES-1 for understanding the role of bcl-2 gene in the carcinogenesis of EBV-associated gastric carcinoma.</p><p><b>METHODS</b>Akata 1061 cells producing recombined EBV carrying neomycin resistance gene (NEOr) was used to mediate the EBV infection of human gastric epithelial cell line GES-1 via close contact, with the empty plasmid pcDNA3-transfected GES-1 cells via lipofectamine method as a control. The EBV-infected and pcDNA3-transfected cells were cloned by limited dilution and the positive clones selected with G418. Immunocytochemical staining was performed to detect the expressions of EBNA1 and Bcl-2 protein.</p><p><b>RESULTS</b>Bcl-2 protein expression was detected in EBV-infected cells but not in the control cells.</p><p><b>CONCLUSION</b>EBV infection can increase Bcl-2 expression in gastric epithelial cells, and such cell transformation effect of EBV is related to the overexpression of bcl-2 gene.</p>