ABSTRACT
OBJECTIVE:To establish a HPLC method for content determination of salvianolic acid B and chrysophenine glycoside in Xiaozhiyin.METHODS:The compounds were base-isolated on the column of Kromesile C 18 at30℃and gradient elution was performed in mobile phase of acetonitrile-0.15%phosphoric acid solution,the wavelength was320nm,and the flow rate was1ml/min.RESULTS:The linearity was obtained between sample size and peak area score over the range of0.8336?g～ 8.336?g(r=1.0000)for salvianolic acid B,and0.352?g～3.52?g(r=1.0000)for chrysophenine glycoside.The average re?covery rate was99.5%(RSD=0.40%)for salvianolic acid B and100.2%(RSD=0.70%)for chrysophenine glycoside.CON?CLUSION:The method is accurate,simple,and highly reproducible,and it can be used for the quality control of Xiaozhiyin.
