Specific recognition of ribavirin in animal-derived foods by high performance liquid chromatography combined with magnetic solid-phase extraction based on highly selective Zr-Fe3 O4.

For the first time, a magnetic solid-phase extraction with high-performance liquid chromatography detection method using Zr functionalized Fe3 O4 magnetic material to enrich ribavirin was successfully established. Zr components that modified in Fe3 O4 nanoparticles via a simple one-step hydrothermal method was selected in this work to specifically capture ribavirin by the strong chemical bonding between Zr components of Zr functionalized Fe3 O4 magnetic material and cis-hydroxyl of ribavirin, which was confirmed by pseudo-second-order kinetic model. And Fe3 O4 components were selected in this work to achieve simple operation. Under the optimal experimental conditions, proposed magnetic solid-phase extraction with high-performance liquid chromatography detection method along with Zr functionalized Fe3 O4 magnetic material offered a wide range linearity at 10-200 µg/L with correlation coefficient of 0.9978 with low detection limit of 2.68 µg/L for ribavirin. The relative standard deviations obtained from nine parallel extractions of 100 µg/L ribavirin were 4.41% and revealed good repeatability. This established method was successfully applied to detect real samples including chicken liver, egg, and shrimp with satisfactory recoveries of 74.13-92.9%.

Study of Efficiency of Coupling Peptides with Gold Nanoparticles / 分析化学

Fluorescence spectroscopy was used to investigate the efficiency of coupling peptides to gold nanoparticles via 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride-N-hydroxysuccinimide (EDC-NHS).The experiment conditions including buffer solution, pH value and concentrations of buffer solution, concentrations of NHS and EDC, concentration ratios of NHS to EDC, and coupling reaction time on the coupling efficiency were investigated and optimized.The experimental results indicated that the optimized experimental conditions were as follows: 25 mmol/L HEPES buffer solution, pH 7.0;2∶1 of concentration ratio of NHS to EDC, 0.4 mol/L NHS, 0.2 mol/L EDC, and coupling reaction time of 24 h.This study may provide references for the relative research involving coupling peptide or protein with gold nanoparticles

Obeservation of curative effects of repetitive transcranial magnetic stimulation therapy on subcortical ischemic vascular disease patients with mild cognitive impairment / 临床神经病学杂志

Objective To explore the curative effects of repetitive transcranial magnetic stimulation ( rTMS) therapy on subcortical ischemic vascular disease( SIVD) patients with mild cognitive impairment.Methods Sixty-one SIVD patients with mild cognitive impairment but did not meet the diagnostic criteria for vascular dementia were enrolled and randomly divided into the treatment group (n=31) and the control group (n=30) in accordance with international common randomization form,TMT-A、TMT-B、VFT、AVLT score and P300 latency and amplitude changes were compared between the two groups before and after treatment.Results TMT-A、TMT-B、VFT、AVLT showed no difference before treatment between the two groups, but improved significantly after treatment in the treatment group ( P=0.040;P=0.041;P=0.034;P=0.010 ) .the results were also significantly different from the control group after treatment( P=0.019;P=0.009;P=0.044;P=0.045 ) .In the treatment group, P300 latency after treatment was significantly reduced than that before treatment (P=0.045),which was also significantly reduced from the control group ( P=0.025 ) , but P300 amplitude did not reach statistically significant difference before and after treatment in the treatment group.In the control group, P300 latency and amplitude did not reach statistically significant difference before and after treatment.Conclusion TMS therapy can improve cognitive function in SIVD with mild cognitive impairment.