ABSTRACT
A series of experiments to study the delayed effects of gamma-radiation exposure in different generations of the progenies of the Chinese hamster ovary CHO-K1 irradiated cells has been conducted. It has been shown that in the progenies of the cells irradiated with a dose of 1 Gy, the following effects are observed: in the 9-27 cell generations - increase in the genome damage, intracellular production of reactive oxygen species, apoptotic cells percentage and cell sensitivity to additional exposure (irradiation at a dose of 10 Gy); in the 30-42 cell generations - decrease of the studied parameters up to control values and increased resistance to additional exposure. It is assumed that the decrease of the studied parameters up to the control values in the 30-42 postirradiation generations of cells is caused by elimination of damaged cells or transition of genomic instability into a hidden (latent) condition.
Subject(s)
Apoptosis/radiation effects , DNA Breaks, Double-Stranded/radiation effects , DNA Fragmentation/radiation effects , Gamma Rays , Genomic Instability/radiation effects , Reactive Oxygen Species/metabolism , Animals , CHO Cells , Cell Culture Techniques , Comet Assay , Cricetinae , CricetulusABSTRACT
The level of damage DNA in neyroblastes of larvae and frequency of recessive sex-linked lethal mutations of males from chronically irradiated populations Drosophila melanogaster, differing on mobile P-elements patterns, was estimated. Received results testify, that exposition in conditions a chronic gamma-radiation (absorbed radiation dose at one generation is compounds 10 mGy) result to increase of significance of parameters and change of sensitivity of cells to following of an acute irradiation in a dose of 3 Gy.
Subject(s)
DNA Breaks, Double-Stranded/radiation effects , Drosophila melanogaster/genetics , Drosophila melanogaster/radiation effects , Gamma Rays , Animals , DNA Transposable Elements/radiation effects , Dose-Response Relationship, Radiation , Ganglia, Invertebrate/radiation effects , Gene Frequency/radiation effects , Genes, Insect/radiation effects , Larva/genetics , Larva/radiation effects , Male , Mutation , Spermatozoa/radiation effectsABSTRACT
We studied histomorphology of the CBA line mice liver after 30 and 90 days of the low dose gamma-radiation influence combined with incorporated Th(NO3)4 in doses 0.03; 0.1; 0.3 grams per kilogram of the living mouse weight. Morphophysiological and morphometric changes were shown. The liver mass and index were significant by increased after 30 days influence. The same changes after 90 days influence were not shown. The morphometric parameters (dynamics of double-nucleus and polyploid hepatocytes, nucleoluses numbers) gave evidence of the liver energy disbalance.
