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Sud Med Ekspert ; 41(2): 40-4, 1998.
Article in Russian | MEDLINE | ID: mdl-9608262

ABSTRACT

The sensitivity of the absorption-elution test (AET) for the detection of A, B, and H antigens in blood traces can be appreciably increased by using serologically active proteases at different stages of the test. A sensitive AET modification is developed, detecting group antigens in far lesser amounts of the material than by routine AET without proteases. A higher sensitivity of the antigen-antibody reaction between enzyme-treated erythrocytes and tested blood traces permits the use of standard group-specific sera less active than usually; the actually solves the problem of deficit of the sera fit for this test.


Subject(s)
ABO Blood-Group System/blood , Blood Stains , Papain , Serine Endopeptidases , Absorption , Antibodies, Heterophile/blood , Antibodies, Monoclonal/blood , Forensic Medicine/methods , Hemagglutinins/blood , Humans , Indicators and Reagents , Sensitivity and Specificity , Solutions
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