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1.
Int Immunopharmacol ; 39: 57-62, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27424079

ABSTRACT

OBJECTIVE: The interaction between nitric oxide (NO) and hydrogen sulfide (H2S) in the airways could have significant implications for the pathogenesis and therapeutic effects of both on lung diseases. In this study we investigated whether the beneficial effects of H2S on asthma could be comparable to that inhibition of inducible NO synthase (iNOS). METHODS: Female BALB/C mice sensitized with ovalbumin (OVA) received either the H2S donor sodium hydrosulfide (NaHS, 14µmol/kg) or the iNOS inhibitor 1400W (1mg/kg), 30min before each OVA challenge during six days. On the first, second and sixth days, the leucocyte infiltration in lung parenchyma and bronchoalveolar lavage was evaluated. The aconitase activity (a sensor of O2 formation) and lipid peroxidation, as well as levels of reduced glutathione (GSH) and oxidized glutathione (GSSG) were determined in the lung tissues. RESULTS: OVA-challenge caused a significant and time-dependent increase in the eosinophil number in the airways, which was accompanied by a significant decrease of aconitase activity and GSH/GSSG ratio along with enhanced lipid peroxidation in the lungs. Treatment with NaHS or 1400W significantly attenuated the airways eosinophilia that was paralleled by an increase in aconitase activity and decrease of lipid peroxidation. NaHS or 1400W treatments also reversed the decreased GSH/GSSG ratio seen after OVA-challenge. CONCLUSIONS: The present study shows for the first time that the increased GSH/GSSG ratio caused by either H2S supplementation or iNOS-inhibition is a potential mechanism protecting airways against oxidative stress and inflammatory lung diseases.


Subject(s)
Asthma/drug therapy , Enzyme Inhibitors/therapeutic use , Glutathione/metabolism , Hydrogen Sulfide/therapeutic use , Lung/drug effects , Neutrophils/drug effects , Pneumonia/drug therapy , Aconitate Hydratase/metabolism , Animals , Cell Movement/drug effects , Enzyme Inhibitors/pharmacology , Female , Lung/immunology , Lung/pathology , Mice , Mice, Inbred BALB C , Neutrophils/immunology , Nitric Oxide Synthase Type II/antagonists & inhibitors , Oxidative Stress/drug effects
2.
Eur J Pharmacol ; 698(1-3): 463-9, 2013 Jan 05.
Article in English | MEDLINE | ID: mdl-23183109

ABSTRACT

Recent studies show that endogenous hydrogen sulfide (H(2)S) plays an anti-inflammatory role in the pathogenesis of airway inflammation. This study investigated whether exogenous H(2)S may counteract oxidative stress-mediated lung damage in allergic mice. Female BALB/c mice previously sensitized with ovalbumin (OVA) were treated with sodium hydrosulfide (NaHS) 30 min before OVA challenge. Forty eight hours after antigen-challenge, the mice were killed and leukocyte counting as well as nitrite plus nitrate concentrations were determined in the bronchoalveolar lavage fluid, and lung tissue was analysed for nitric oxide synthase (NOS) activity, iNOS expression, superoxide dismutase (SOD), catalase, glutathione reductase (GR) and glutathione peroxidase (GPx) activities, thiobarbituric acid reactive species and 3-nitrotyrosine containing proteins (3-NT). Pre-treatment of OVA-sensitized mice with NaHS resulted in significant reduction of both eosinophil and neutrophil migration to the lungs, and prevented the elevation of iNOS expression and activity observed in the lungs from the untreated allergic mice, although it did not affect 3-NT. NaHS treatment also abolished the increased lipid peroxidation present in the allergic mouse lungs and increased SOD, GPx and GR enzyme activities. These results show, for the first time, that the beneficial in vivo effects of the H(2)S-donor NaHS on allergic airway inflammation involve its inhibitory action on leukocyte recruitment and the prevention of lung damage by increasing endogenous antioxidant defenses. Thus, exogenous administration of H(2)S donors may be beneficial in reducing the deleterius impact of allergic pulmonary disease, and might represent an additional class of pharmacological agents for treatment of chronic pulmonary diseases.


Subject(s)
Hydrogen Sulfide/pharmacology , Hypersensitivity/metabolism , Lung/drug effects , Lung/metabolism , Oxidative Stress/drug effects , Animals , Catalase/metabolism , Female , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Hypersensitivity/enzymology , Hypersensitivity/immunology , Leukocytes/drug effects , Leukocytes/immunology , Lung/enzymology , Lung/immunology , Mice , Mice, Inbred BALB C , Nitric Oxide Synthase/metabolism , Sulfides/pharmacology , Superoxide Dismutase/metabolism , Thiobarbiturates/metabolism , Tyrosine/analogs & derivatives , Tyrosine/metabolism
3.
Free Radic Biol Med ; 47(10): 1432-9, 2009 Nov 15.
Article in English | MEDLINE | ID: mdl-19703550

ABSTRACT

We have recently demonstrated that hypertriglyceridemic (HTG) mice present both elevated body metabolic rates and mild mitochondrial uncoupling in the liver owing to stimulated activity of the ATP-sensitive potassium channel (mitoK(ATP)). Because lipid excess normally leads to cell redox imbalance, we examined the hepatic oxidative status in this model. Cell redox imbalance was evidenced by increased total levels of carbonylated proteins, malondialdehydes, and GSSG/GSH ratios in HTG livers compared to wild type. In addition, the activities of the extramitochondrial enzymes NADPH oxidase and xanthine oxidase were elevated in HTG livers. In contrast, Mn-superoxide dismutase activity and content, a mitochondrial matrix marker, were significantly decreased in HTG livers. Isolated HTG liver mitochondria presented lower rates of H(2)O(2) production, which were reversed by mitoK(ATP) antagonists. In vivo antioxidant treatment with N-acetylcysteine decreased both mitoK(ATP) activity and metabolic rates in HTG mice. These data indicate that high levels of triglycerides increase reactive oxygen generation by extramitochondrial enzymes that promote mitoK(ATP) activation. The mild uncoupling mediated by mitoK(ATP) increases metabolic rates and protects mitochondria against oxidative damage. Therefore, a biological role for mitoK(ATP) as a redox sensor is shown here for the first time in an in vivo model of systemic and cellular lipid excess.


Subject(s)
Hypertriglyceridemia/metabolism , Mitochondria, Liver/metabolism , Potassium Channels/metabolism , Animals , Disease Models, Animal , Female , Male , Mice , Mice, Transgenic , Oxidation-Reduction , Reactive Oxygen Species/metabolism
4.
Free Radic Res ; 43(4): 340-7, 2009 Apr.
Article in English | MEDLINE | ID: mdl-19212855

ABSTRACT

H2O2 can freely crosses membranes and in the presence of Fe2+ (or Cu+) it is prone to participate in Fenton reaction. This study evaluated the concentration and time-dependent effects of H2O2-induced oxidative stress on MnSOD, Se:GPx and catalase and on aconitase. Acute and chronic H2O2 treatments were able to induce oxidative stress in HeLa cells as they significantly decreased aconitase activity and also caused a very significant decrease on antioxidant enzyme activities. The inhibition of enzyme activities was time- and concentration-dependent. Chronic treatment with 5 microM H2O2/h after 24 h was able to decrease all enzyme activities almost at the same level as the acute treatment. Acute and chronic treatments on antioxidant enzyme activities were prevented by cell treatment with ascorbic acid or N-acetylcysteine. These results indicate that antioxidant enzymes can also be affected by the same ROS they produce or neutralize if the time of exposure is long enough.


Subject(s)
Antioxidants/metabolism , Hydrogen Peroxide/toxicity , Oxidative Stress/drug effects , Acetylcysteine/pharmacology , Aconitate Hydratase/metabolism , Antioxidants/pharmacology , Ascorbic Acid/pharmacology , Catalase/metabolism , Glutathione Peroxidase/metabolism , HeLa Cells , Humans , Hydrogen Peroxide/administration & dosage , Kinetics , Superoxide Dismutase/metabolism
5.
J Enzyme Inhib Med Chem ; 22(4): 439-44, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17847710

ABSTRACT

Flavonoids, polyphenolic phytochemicals, are ubiquitous in plants and are commonly present in the human diet. They may exert diverse beneficial effects, including antioxidant and anticarcinogenic activities. The present study was designed to evaluate three biomolecules that play important roles in the apoptotic process: mitogen-activated protein kinases, protein phosphatases and NFkappaB, using HL60 cells treated with fisetin as an experimental model. Our results demonstrated that cells treated with fisetin presented high expression of NFkappaB, activation of MAPK p38 and an increase of phosphoprotein levels; inhibition of enzymes involved in redox status maintenance were also observed. Our findings reinforce the hypothesis that fisetin is likely to exert beneficial and/or toxic actions on cells not through its potential as antioxidant but rather through its modulation of protein kinase and phosphatase signaling cascades. Additionally, our results also indicate that the cellular effects of fisetin will ultimately depend on the cell type and on the extent to which they associate with the cells, either by interactions at the membrane or by uptake into the cytosol.


Subject(s)
Flavonoids/metabolism , MAP Kinase Signaling System , NF-kappa B/biosynthesis , Phosphoproteins/chemistry , Antioxidants/chemistry , Antioxidants/metabolism , Cytosol/metabolism , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Flavonols , HL-60 Cells , Humans , Lymphocytes/metabolism , Models, Chemical , Oxidative Stress , Phosphoproteins/metabolism , Phosphorylation , p38 Mitogen-Activated Protein Kinases/metabolism
6.
Clin Biochem ; 40(9-10): 615-22, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17466292

ABSTRACT

OBJECTIVES: To analyze the status of expression of inflammation markers, antioxidant and oxidant enzymes in biopsies from patients diagnosed with gastritis, gastric ulcer (GU) and gastric cancer (GC) and the Helicobacter pylori virulence from these isolated biopsies in order to evaluate a possible association among these factors. METHODS: H. pylori genotype from isolated biopsies was performed by PCR. The pattern of expression of inflammation (TNF-alpha, IL-1beta, IL-8, IL-10 and IL-12), oxidant (iNOS and Nox1) and antioxidant markers (MnSOD, GPX and CAT) of biopsies from gastritis, GU, GC and control groups was performed by RT-PCR. RESULTS: Different from other gastric diseases studied here, gastritis is characterized by an oxidative stress with significant expression of TNF-alpha, IL-8, IL-12, iNOS and Nox and significant absence of MnSOD and GPX expression. Gastritis was the only condition where there was an association between TNF-alpha or IL-8 expression and H. pylori cagA+/vacAs1 genotype. In this case, TNF-alpha expression was about 3 times higher when compared to control subjects. CONCLUSION: In this study, only gastritis was found to be associated with significant oxidative stress marker expression of TNF-alpha and IL-8 that was also related to H. pylori virulence, suggesting that they are the main oxidant stress markers responsible to trigger an increase in ROS level that contributes to decrease the expression of the MnSOD and GPX.


Subject(s)
Helicobacter pylori/pathogenicity , Interleukin-8/blood , Oxidative Stress/physiology , Stomach Diseases/physiopathology , Tumor Necrosis Factor-alpha/blood , Antioxidants/metabolism , Gastritis/physiopathology , Gene Expression , Helicobacter Infections/physiopathology , Helicobacter pylori/genetics , Helicobacter pylori/isolation & purification , Humans , Interleukin-10/blood , Interleukin-12/blood , Multienzyme Complexes/blood , NADH, NADPH Oxidoreductases/blood , Nitric Oxide Synthase Type II/blood , Stomach Diseases/microbiology , Stomach Neoplasms/physiopathology , Stomach Ulcer/physiopathology , Virulence
7.
Arch Biochem Biophys ; 458(2): 229-35, 2007 Feb 15.
Article in English | MEDLINE | ID: mdl-17224128

ABSTRACT

We report that two fractions containing proteins from rat hepatocyte nuclei, obtained by nondenaturing gel electrophoresis, were able to bind iron and ATP, and to hydrolyze ATP. Electroelution of these two active fractions followed by SDS-PAGE analysis showed an identical protein pattern, each one containing four proteins in a range of 62-80 kDa. Phosphorylated protein bands were also detected in acid gel and disappeared after treatment with hydroxylamine/acetate or KOH, and upon chasing with cold ATP. A proteoliposome system, made by the incorporation of these partially purified protein fractions into phosphatidylcholine vesicles, carried out Fe(3+)-citrate uptake in a Mg(2+)-ATP-dependent way; Fe(3+) accumulation increased with time reaching a plateau in 30 min. Iron uptake was not supported by AMP-PNP, was partially inhibited by orthovanadate and was not affected by a mix of specific inhibitors of known ATPases. These results support our previous hypothesis that a putative nuclear membrane Fe(3+)-ATPase is involved in nuclear iron homeostasis.


Subject(s)
Adenosine Triphosphatases/metabolism , Ferric Compounds/metabolism , Proteolipids/metabolism , Adenosine Triphosphatases/isolation & purification , Adenosine Triphosphate/metabolism , Animals , Cell Nucleus/metabolism , Electrophoresis, Polyacrylamide Gel , Hepatocytes/ultrastructure , Nuclear Envelope/enzymology , Nuclear Proteins/isolation & purification , Nuclear Proteins/metabolism , Phosphorylation , Rats
8.
FEMS Immunol Med Microbiol ; 38(3): 193-8, 2003 Oct 15.
Article in English | MEDLINE | ID: mdl-14522454

ABSTRACT

Infection by Helicobacter pylori elicits persistent neutrophil infiltration in the gastric mucosa and stimulates the release of substances that may contribute to the establishment of gastritis. In this study, we used the rat air pouch model to evaluate the acute inflammatory response to H. pylori, in vivo. A pronounced neutrophil infiltration was observed 6 h and 12 h after the injection of H. pylori into the air pouch. Strains with different genotypes were able to induce cellular influx. This response was dependent upon the amount of bacteria injected and still occurred when heat-killed bacteria were employed. An increase in prostaglandin E(2) levels was observed, indicating that H. pylori induced cyclooxygenase 2 in this model. The production of interleukin-1 beta and tumor necrosis factor-alpha by leukocytes was also enhanced, suggesting that this model may be useful for studying the direct activation of neutrophils by H. pylori in vivo.


Subject(s)
Helicobacter Infections/physiopathology , Helicobacter pylori/pathogenicity , Inflammation , Animals , Dinoprostone/analysis , Disease Models, Animal , Exudates and Transudates/chemistry , Exudates and Transudates/immunology , Helicobacter Infections/immunology , Helicobacter Infections/microbiology , Helicobacter Infections/pathology , Interleukin-1/analysis , Leukocyte Count , Leukocytes , Male , Neutrophil Infiltration , Rats , Rats, Wistar , Tumor Necrosis Factor-alpha/analysis
9.
Environ Health Perspect ; 110(8): 749-55, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12153754

ABSTRACT

In vitro studies suggest that reactive oxygen species contribute to the cardiopulmonary toxicity of particulate air pollution. To evaluate the ability of particulate air pollution to promote oxidative stress and tissue damage in vivo, we studied a rat model of short-term exposure to concentrated ambient particles (CAPs). We exposed adult Sprague-Dawley rats to either CAPs aerosols (group 1; average CAPs mass concentration, 300 +/- 60 micro g/m3) or filtered air (sham controls) for periods of 1-5 hr. Rats breathing CAPs aerosols for 5 hr showed significant oxidative stress, determined as in situ chemiluminescence in the lung [group 1, 41 +/- 4; sham, 24 +/- 1 counts per second (cps)/cm2] and heart (group 1, 45 +/- 4; sham, 24 +/- 2 cps/cm2) but not liver (group 1, 10 +/- 3; sham, 13 +/- 3 cps/cm2). Increases in oxidant levels were also triggered by highly toxic residual oil fly ash particles (lung chemiluminescence, 90 +/- 10 cps/cm2; heart chemiluminescence, 50 +/- 3 cps/cm2) but not by particle-free air or by inert carbon black aerosols (control particles). Increases in chemiluminescence showed strong associations with the CAPs content of iron, manganese, copper, and zinc in the lung and with Fe, aluminum, silicon, and titanium in the heart. The oxidant stress imposed by 5-hr exposure to CAPs was associated with slight but significant increases in the lung and heart water content (approximately 5% in both tissues, p < 0.05) and with increased serum levels of lactate dehydrogenase (approximately 80%), indicating mild damage to both tissues. Strikingly, CAPs inhalation also led to tissue-specific increases in the activities of the antioxidant enzymes superoxide dismutase and catalase, suggesting that episodes of increased particulate air pollution not only have potential for oxidant injurious effects but may also trigger adaptive responses.


Subject(s)
Air Pollutants/adverse effects , Inhalation Exposure , Oxidative Stress , Reactive Oxygen Species/analysis , Aerosols , Animals , Dose-Response Relationship, Drug , Lung/chemistry , Male , Myocardium/chemistry , Particle Size , Rats , Rats, Sprague-Dawley
10.
Ciênc. cult. (Säo Paulo) ; 47(5/6): 320-4, Sept.-Oct. 1995. ilus
Article in English | LILACS | ID: lil-186418

ABSTRACT

The interplay between cellular iron homeostasis and metabolism of reactive oxidative species is reviewed, mainly from the viewpoint of the possible consequences for DNA damage inflicted by these species. It is shown that genetic manipulation of the iron homeostasis gene repertoire affects directly the response of DNA to the aggression by oxidant species. It is also shown that a condition of oxidative stress alters iron homeostasis, providing the perception that these two events are mutually dependent. The presence of iron in the nucleus is reviewed and new data are discussed pointing both to i) the participation of iron as a ligand of an underined chromatin component, and to ii) mechanisms of active transport of iron into the nucleus. The question of which mechanism is more important for DNA strand breaks under oxidative stress, if calcium-activated nuclease or .OH radical generated by the Fenton reaction is discussed. New data are reviewed showing that the chemical nature of the 3'-terminus at the scission point confirms the .OH radical attack mechanism. Finally, genetic manipulation experiments at the level of metallothionein and superoxide dismutase genes allowed to engineer cells that provided important information: i) Metallothionein seems to be a nuclear antioxidant protein, playing a protective role against attack to DNA and ii) the CU/Zn superoxide dismutase balance is a very crucial one; an excess of this enzyme may downregulate the synthesis of antioxidant proteins, rendering the cells more vulnerable to oxidant attack.


Subject(s)
DNA Damage , Iron/metabolism , Reactive Oxygen Species/metabolism
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