[Vaccine prophylaxis of viral hepatitis B in patients with acute leukemia].

AIM: To assess efficacy of vaccine against viral hepatitis B (VHB) in adults after achievement of acute leukemia (AL) remission. MATERIAL AND METHODS: Specific prevention of VHB was made in 30 AL patients with recombinant vaccine Engerix B by two schemes: 0-1-2-12 and 0-1-2-6 months with double adult dose 40 mcg. Efficacy of vaccination was evaluated by the titer of antibodies to HBs-Ag (HBs-Ab) 1 month after each injection of the vaccine. The control group consisted of 36 patients. Follow-up was 6 months to 10 years. RESULTS: Vaccine prophylaxis of VHB in AL remission resulted in appearance of the protective antibody titer after the forth vaccine injection in 50% patients. Out of 30 vaccinated patients 2 (6.7%) got infected with HBV 3 and 5 years after onset of the disease. Markers of HBV replication were detected in 8 (22%) of 36 patients. CONCLUSION: Vaccine prophylaxis of VHB in adult patients in AL remission was effective in 93.3% of the vaccinated patients.

[The role of specific blood serum factors in disrupting insulin binding to erythrocyte cell membrane receptors]. / Rol' spetsificheskikh faktorov syvorotki krovi v narushenii sviazyvaniia insulina s retseptorami plazmaticheskikh membran éritrotsitov.

The alteration of indices of 125-I insulin binding to receptors in the reactions with human erythrocytes, with their membranes and with solubilized insulin receptors in the presence of type 2 diabetics' blood serum was demonstrated (decrease of the number of insulin binding sites and increase of the dissociation constant). Treatment of patients' erythrocytes with acetate buffer solution (0.1 M, pH 5, 150 mM sodium chloride) restored the level of specific binding of insulin to normal values. It is supposed that in insulin-independent diabetes mellitus patients' serum there are specific blocking factors which interact immediately with insulin receptors thus preventing them from hormone binding or reducing their affinity.

[Biochemical and physico-chemical changes in albumin in dynamics of experimental allergic encephalomyelitis]. / Biokhimichni ta fizyko-khimichni zminy al'buminu v dynamitsi eksperymental'nogo alergichnogo entsefalomiielitu.

The experiments on guinea pig with experimental allergic encephalomyelitis (EAE) have shown that the most significant changes in the content, binding ability and thermodynamic characteristics of serum albumin are found at the early stages preceding the appearance of neurological characters. The plasma perfusion through granulated carbon-activated sorbents increases the binding ability and decreases the temperature denaturation of albumin down to the normal level.

Effect of hemoperfusion on the course of demyelinization processes: experimental and clinical picture.

The experiments on guinea pigs with experimental allergic encephalomyelitis showed, that hemoperfusion through fibrous and granulated activated carbon resulted in stable clinical effect solely on the early stages of the disease (10-11 days after sensitization). At the stage of advanced neurologic symptomatology the hemoperfusion proved effective when performed through binary adsorbent on the base of fibrous activated carbon and immobilized basic encephalitogenous protein. Forty nine patients with multiple sclerosis underwent 2-3 sessions of hemocarboperfusion on SKN coal with 7 day intervals. In patients at the initial stage of the disease the hemocarboperfusion was found to produce a stable and practically complete normalizing effect on the neurologic status. At the advanced stages of the disease the hemosorption eliminates only newly developed symptoms.

[Effect of alpha-tocopherol on relations between RNA and ubiquinone biosynthesis in rat liver]. / Vzaimosviaz' izmenenii biosinteza RNK i ubikhinona v pecheni krys pod vozdeistviem alpha-tokoferola.

The animals were kept on the low-protein diet deprived of vitamin E. Addition of the latter to the diet fed to a noticeable increase in the intensity of the biosynthesis of total and nuclear RNA of the rat liver. alpha-Tocopherol had a clearly marked stimulant effect on the synthesis of RNA and ubiquinone as shown by in vitro experiments under 30-minute preincubation of the liver from rats with E-hypovitaminosis. Meanwhile actinomycin D was found to inhibit these processes. Under preincubation with actinomycin D or concurrent introduction of the antibiotic with alpha-tocopherol the effect of the latter does no get realized. Unlike alpha-tocopherol, ionol and other synthetic antioxidants proved ineffective. It is assumed that the stimulant effect of vitamin E on the biosynthesis of ubiquinone in the liver of rats with E-hypovitaminosis is related to the increased intensity of the RNA synthesis and differs from the antioxidant one as regards the type. It is also assumed that the action of alpha-tocopherol on the RNA synthesis is effected at the transcriptional level.

[Possibility of preventing muscular dystrophy in E avitaminosis with alpha-tocopheronolactone and diludin]. / Izuchenie vozmozhnosti preduprezhdeniia myshechnoi distrotii pri E-avitaminoze al'fa-tokoferonolaktonom i diludinom.

The efficacy of pharmacopeial alpha-tocopheryl acetate, alpha-tocopheronolactone and the antioxidant diludin was studied as to the possibilities of preventing E-hypovitaminosis muscle dystrophy in rabbits. alpha-Tocopheronolactone action was similar to that of pharmacopeial alpha-tocopheryl acetate as regards E-vitamin activity that was manifested by an increase in the body mass, decrease in the urine creatine index and maintenance of normal ubiquinone content, as well as of succinate-ubiquinone-reductase, NADH-ubiquinone-reductase and NADH-cytochrome c-reductase activity of rabbit skeletal muscle and heart mitochondria. On the contrary , as a result of the antioxidant administration, no direct correlation was found between ubiquinone content and activity of ubiquinone-dependent enzymatic systems of mitochondria. The data obtained attest to high E-vitamin activity of alpha-tocopheronolactone. The action of the latter was disclosed to be different from that produced by the antioxidant.

[Alpha-tocopherol and actinomycin D regulation of ubiquinone and protein biosynthesis in rat liver in E-hypovitaminosis]. / Reguliatsiia al'fa-tokoferolom i aktinomitsinom D biosinteza ubikhinona i belka v pecheni krys pri E-gipovitaminoze.

In vitro experiments with the liver preparations from rats with E-hypovitaminosis revealed a more intensive incorporation of [14C]phenylalanine into ubiquinone induced by alpha-tocopherol. Actinomycin D inhibiting the DNA-dependent RNA synthesis decreases the incorporation intensity. A 30 min preincubation of the liver preparations with thesis. In case of preliminary addition of actinomycin D or its simultaneous introduction with alpha-tocopherol the latter has no stimulating effect on the intensity of [14C] phenylalanine incorporation into ubiquinone. When studying incorporation of [14C]phenylalanine into the total protein of the liver of rats with E-hypovitaminosis a tendency is revealed to an increase in the protein biosynthesis intensity in case of preincubation with alpha-tocopherol and to inhibition of the labelled precursor incorporation under conditions of preincubation with actinomycin D. The data obtained permit suggesting that the alpha-tocopherol effect on the ubiquinone biosynthesis may occur due to its effect on the RNA synthesis.