[Changes in the neuronal ultrastructure of the plexuses of the small intestine in suckling rabbits with experimental cholera]. / Izmeneniia ul'trastruktury neironov spletenii tonkoi kishki krolikov-sosunkov pri éksperimental'noi kholere.

Ultrastructural changes in the small intestine nerve cells have been studied in 34 suckling rabbits infected intragastrically with V. cholerae 01. A correlation between neuron reactions and vascularization of the intramural ganglia have been revealed. Initial alterations (1.5 hr) in nerve cells were characterized by adoptive reorganization of the cytoplasmic organelle. During the period of V. cholerae adhesion (4 hr) ultrastructural alterations were accompanied by cytoplasmic chromatolysis. 1-2 days later, i.e. during the period of clinical manifestations of experimental cholera, changes in the ultrastructure of neurons became stable and most of the injuries were irreversible. The involvement of small intestine nerve elements in this process may be one of pathogenetic links of cholera infection.

[Ultrastructural bases of microcirculatory disorders in intraganglionic capillaries and disorders of nerve elements in the small intestine of suckling rabbits in experimental cholera]. / Ul'trastrukturnye osnovy mikrotsirkuliatornykh rasstroistv v intraganglionarnykh kapilliarakh i narushenii nervnykh élementov tonkoi kishki krolikov-sosunkov pri éksperimental'noi kholera.

The 10-12-day suckling rabbits received culture of V. el tor into the stomach, and the ultrastructural changes of intraganglionic capillaries and nervous elements of small intestine were studied in the course of experimental cholera. It was established that the initial changes 1.5 hrs after the beginning of the experiment were characterized by permeability increase, thrombohemorrhagic syndrome development, oedema of endotheliocytes and perivascular space, fibrin precipitation. There were reactive changes in neurons, but alterations in synapses were absent. In the period of cholera vibrio adhesion (4 hrs) the new signs of endothelial cells desquamation developed. At the same time haemorrhages became more distinct and cells of extravasates more diverse. The development of experimental cholera (1-2 days) was accompanied by appearance of large numbers of coated vesicles in endothelium, tight junctions damage and endothelial cells destruction, extensive dysendothelization which promoted blood cells contact with collagen. In this period dystrophic changes progressed in neurons and activation of cholinergic synapses took place. Enhancement of transepithelial transport of liquid, induced by cholera toxin, is partly connected with activation of cholinergic mechanisms in autonomic ganglia of the small intestine.

[Ultrastructural changes in interneuron connections in intramural ganglia of the small intestine in suckling rabbits with experimental cholera]. / Ul'trastrukturnye izmeneniia mezhneironnykh sviazei v intramural'nykh gangliiakh tonkoi kishki krolikov-sosunkov pri éksperimental'noi kholere.

Data, received in investigation of the small intestine intramural ganglia of 34 suckling rabbits show, that development of experimental cholera induce in the neurons reactive shifts, changed by dystrophic alterations. Prevaluation of cholinergic influences in autonomic ganglia in small intestine promotes enterocytes hypersecretion and accelerating of motor activity.