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1.
Mol Cell ; 68(5): 978-992.e4, 2017 Dec 07.
Article in English | MEDLINE | ID: mdl-29198561

ABSTRACT

Covalent nucleotide modifications in noncoding RNAs affect a plethora of biological processes, and new functions continue to be discovered even for well-known modifying enzymes. To systematically compare the functions of a large set of noncoding RNA modifications in gene regulation, we carried out ribosome profiling in budding yeast to characterize 57 nonessential genes involved in tRNA modification. Deletion mutants exhibited a range of translational phenotypes, with enzymes known to modify anticodons, or non-tRNA substrates such as rRNA, exhibiting the most dramatic translational perturbations. Our data build on prior reports documenting translational upregulation of the nutrient-responsive transcription factor Gcn4 in response to numerous tRNA perturbations, and identify many additional translationally regulated mRNAs throughout the yeast genome. Our data also uncover unexpected roles for tRNA-modifying enzymes in regulation of TY retroelements, and in rRNA 2'-O-methylation. This dataset should provide a rich resource for discovery of additional links between tRNA modifications and gene regulation.


Subject(s)
RNA, Fungal/metabolism , RNA, Transfer/metabolism , Ribosomes/enzymology , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/enzymology , Transcriptome , tRNA Methyltransferases/metabolism , Basic-Leucine Zipper Transcription Factors/biosynthesis , Basic-Leucine Zipper Transcription Factors/genetics , Gene Expression Profiling/methods , Gene Expression Regulation, Fungal , Genotype , Methylation , Mutation , Phenotype , RNA, Fungal/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Ribosomal/genetics , RNA, Ribosomal/metabolism , RNA, Transfer/genetics , RNA, Untranslated/genetics , RNA, Untranslated/metabolism , Retroelements , Ribosomes/genetics , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae Proteins/biosynthesis , Saccharomyces cerevisiae Proteins/genetics , Terminal Repeat Sequences , tRNA Methyltransferases/genetics
2.
Cell Rep ; 21(13): 3807-3818, 2017 12 26.
Article in English | MEDLINE | ID: mdl-29281829

ABSTRACT

Aneuploidy disrupts cellular homeostasis. However, the molecular mechanisms underlying the physiological responses and adaptation to aneuploidy are not well understood. Deciphering these mechanisms is important because aneuploidy is associated with diseases, including intellectual disability and cancer. Although tumors and mammalian aneuploid cells, including several cancer cell lines, show altered levels of sphingolipids, the role of sphingolipids in aneuploidy remains unknown. Here, we show that ceramides and long-chain bases, sphingolipid molecules that slow proliferation and promote survival, are increased by aneuploidy. Sphingolipid levels are tightly linked to serine synthesis, and inhibiting either serine or sphingolipid synthesis can specifically impair the fitness of aneuploid cells. Remarkably, the fitness of aneuploid cells improves or deteriorates upon genetically decreasing or increasing ceramides, respectively. Combined targeting of serine and sphingolipid synthesis could be exploited to specifically target cancer cells, the vast majority of which are aneuploid.


Subject(s)
Aneuploidy , Saccharomyces cerevisiae/metabolism , Serine/metabolism , Sphingolipids/biosynthesis , Cell Proliferation , Ceramides/metabolism , Membrane Proteins/metabolism , Mitochondria/metabolism , Serine/biosynthesis , Transcription, Genetic , Up-Regulation/genetics
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