[Reproductive toxicological fundamental investigations on adult male rats of the WIST/Lppt stock on the determination of swelling after administration of cadmium chloride]. / Reproduktionstoxikologische Grundlagenuntersuchungen an adulten männlichen Ratten des Stammes WIST/Lppt zur Schwellenwertbestimmung nach Cadmiumchloridapplikation.

The reproductive-toxic effects after an oral administration of Cadmium as Cadmium chloride on testes and epididymidis of mature Wistar rats were investigated. After daily dosages of 0.3, 0.6, 1.0 oder 2.0 mg CdCl2/kg b.w. over one or two weeks no changes of testes and epididymidis were detected in comparison with control animals by histological observations. By means of morphometrical methods significant changes of the testes from a dosage of 1.0 mg/kg b.w. and changes of the epididymidis previously a dosage of 0.3 mg/kg b.w. have been demonstrated. That means the effect level of Cadmium is higher by consideration of testes than of epididymidis. Consequently, during investigations of reproductive toxicity one hand to test both organs.

[Histological and histochemical investigations on the structure of udder skin of cattle with special reference to changes during in vivo udder perfusion models]. / Histologisch-histochemische Untersuchungen zum Bau der Euterhaut des Rindes unter besonderer Berücksichtigung der Veränderungen durch die Perfusion im Rahmen des Eutermodells.

Generally, the skin of the bovine mammary gland is found to be similar to the skin of the integument. In the epidermis, the stratum lucidum is lacking. A prominent stratum corneum can be observed as well as in the haired areas and in areas without hairs as well. Distinct granules of melanin are located mainly in the stratum basale in pigmented skin areas. By means of the alkaline phosphatase, dendritc cells can be seen in the basal parts of the epidermis. In the corium, collagenous fibres are less thick below the epithelium, whereas coarse bundles are seen deeper in the stratum reticulare. The appearance of multilobal sebaceous glands and apocrine sweat glands is associated with a hair follicle, which occur loosely distributed as single hairs. A large erector pili muscle is situated on the side of the hair slope. The numerous sweat glands with wide diameters appear below the hair papilla, and vary greatly in shape and size. Myoepithelials cells are seen at the periphery of its secretory portions. The small number of differences in all areas of the skin examined, are reduced to differences between haired areas and the base of the teats. The histologic structure of the epidermis remains unchanged by the perfusion. A partly collagenolysis occur in the dermis and subcutis.

[Histomorphometric and histological investigations of the morphometric effects of furazolidone on spermatogenesis in mature rats]. / Histomorphometrische und histologische Untersuchungen zur morphokinetischen Wirkung von Furazolidon auf die Spermiogenese geschlechtsreifer Ratten.

The antispermatongenic effects of furazolidone on the testes of mature Wistar rats were investigated using histological and morphometric methods. The sections showed a varying degree of depopulation of the germinal epithelium, a shrinking and a deformation of the Tubuli contorti, and an enlargement of the intertubular lymphatic sinuses. The strain led to a standstill in the spermatogenesis at the primary-spermatocyte stage. After administering furazolidone, the following results could be seen: a weight loss of up to 42.1% and a decrease in testes volume by up to 30.2%; a decrease in the volume of the nuclei of the Leydig-cells by up to 51.6%; a reduction in the diameter, perimeter and area covered by Tubuli seminiferi contorti of up to 33.5%, 30.8%, and 53.4%, respectively; an increase in the number of Tubuli seminiferi contorti per mm2 by up to 44.8%; a decrease in the percentage of Tubuli seminiferi contorti of the total testes tissue by up to 39.1%.

[Basic reproductive toxicological studies of the effect of furazolidone on the hypothalamo-hypophyseo-gonadal axis of male rats]. / Reproduktionstoxikologische Grundlagenuntersuchungen zur Wirkung von Furazolidon auf die Achse Hypothalamus--Hypophyse-Gonaden der männlichen Ratte.

The reproductive-toxic effects of furazolidone on testis, epididymis and selected nuclei of the hypothalamus of male wistar rats were investigated. After a dosage of 50 mg/kg b.w. daily over five days no changes of testis and epididymis were detected in relation to controls by means of light microscope. Using morphometric methods a significant increase of the nucleus volume of the measured nuclei of the hypothalamus (Ncl. ventromedialis, Ncl. praeopticus medialis, Ncl. infundibularis) could be shown. After the high dosage of 200 mg/kg b.w. daily for five days testis and epididymis showed marked signs of atrophy and the volume of the investigated nuclei of the hypothalamus was significantly increased. These results suggest that--contrary to the wide spread opinion the testis would react most sensitive to chemical agents--more attention should be directed to the hypothalamus in reproductive-toxicological studies.

[Histologic and histometric studies of the epiphysis cerebri of the fattening guinea fowl (Meleagris gallopavo) after the use of different light regimens]. / Histologische und histometrische Untersuchungen an der Epiphysis cerebri von Mastputen (Meleagris gallopavo) nach Anwendung unterschiedlicher Lichtregime.

Chicken turkey-hens aged 8 weeks were exposed to a light regime of 1 hour light to 2 hours darkness for 8 weeks. Another experimental group was exposed to permanent lighting from hatching to the 16th week of age. The epiphyses of 5 males and females of either group were histologically, histochemically, and histometrically investigated. Significant differences were found to exist between the 2 experimental groups with regard to the nuclear volumes of epiphyseal cells. Epiphyseal cell activity was found to be reduced by permanent lighting.

[Morphological changes in the pineal body of domestic swine (Sus scrofa domesticus) depending on light]. / Morphologische Veränderungen an der Epiphysis cerebri von Hausschweinen (Sus scrofa domesticus) in Abhängigkeit vom Licht.

The cerebral epiphysis of twelve gilts, aged 6.5 months, was histologically, histochemically, and histometrically tested, after the animals had been kept permanently under artificial light, 100 lux, or in darkness, interrupted twice a day by two hours of lighting, over ten weeks altogether. The parenchymal cells of the epiphyses of the animals kept in darkness changed clearly from those of the other group with constant exposure to light. Changes in the darkness animals included significant enlargement of the cell nuclear volume by 33 per cent, increase in nucleoli count as well as increases of lipids and ribonucleic acid in cytoplasm.

[Histochemical investigation on the parenchyma cells of the Epiphysis cerebri of cattle (Bos taurus domesticus) and pig (Sus scrofa domesticus) (author's transl)]. / Histochemische Untersuchungen an den Parenchymzellen der Epiphysis cerebri von Rind (Bos taurus domesticus) und Schwein (Sus scrofa domesticus).

The parenchyma cells of the pineal organ from 20 cattle (10 week-old female calfs, from one year to several years of age female and male cattle) and 14 pigs (8- to 9-month-old female pigs) were investigated with histochemical methods. Carbohydrates are evident in a insignificant quantity. The content of lipids is very high, according to the age the quantity varying. Proteins are evident, but only in small quantities. The content of ribonucleic acid is very high.

[Studies on the orthology of epiphysis cerebri in cattle (Bos taurus var. domesticus L.)]. / Untersuchungen zur Orthologie der Epiphysis cerebri beim Rind (Bos taurus var. domesticus L.).

The pineal organ from 20 cattle (10 week-old female calfs, from one year to several years of age male and female cattle) were investigated with histological and histochemical methods. Carbohydrats are evident in a insignificant quantity in the parenchyma cells. The content of lipids is very high according to the age of the cattle the quantity varying. Proteins are evident only in small quantities. The content of ribonucleic acid is equally high in the cytoplasma of the parenchyma cells.

[The subcapsular blastema of the adrenal cortex of the swine after continuous long-term infusion of exogenous ACTH]. / Das Verhalten des subkapsulären Blastems der Nebennierenrinde des Schweines nach kontinuierlicher Langzeitdauerinfusion von exogenem

After long time application of homologous ACTH the morphokinesis of the adrenal cortex of the pig was investigated experimentally. Following results were obtained: 1. In view of the controls the absolute and relative weight of the adrenals is raised considerably. 2. The progressive transformation is followed by the disappearance of the zonal structure of the adrenal cortex, and the parenchyma get the picture of fasciculata cells generally. 3. Nearly exclusive the zona fasciculata consists of great, pale activated spongiocytes with 2 nucleoli frequently. Topochemically glycogen and the lipids are inconstant, however the histochemical activity of succinodehydrogenase, lactate dehydrogenase, acid and alkaline phosphatase are considerable raised in regard of the controls. 4. The zona fasciculata contains degenerated cells isolated only. Signs of extensive regressive changes are not present. 5. The zona glomerulosa is dissolving or eliminating respectively. The consequences for the synthesis of the adrenal steroid hormones are discussed. 6. A large, spongy subcapsular blastema with several cell layers and a rich capillary network develop between the fibrous capsula of the adrenal and the zona fasiculata. The fasciculata cells are the direct continuation of the subcapsular blastema. The blastema contains neither glycogen nor lipids and histochemical activities of the enzymes are absent, too. The significance of the subcapsular blastema for the morphological and functional adaptation of the adrenal cortex in stress are discussed. Under the conditions of the closed hypothalamo-hypophyseal-adrenal control system the new origin of cells (hyperplasia) is not significant for the morphokinetic adaptive reactions of the adrenal cortex. Rather the subcapsular blastema represents a reserve area which after the destruction of the endocrine parenchyma through specific pathogens the organism enabled to the regeneration of the adrenal cortex.