ABSTRACT
Allergy to pollen of Cupressaceae has been linked to pollens of Cupressus, Juniper and Cryptomeria. The authors report 2 cases of rhinitis and conjunctivitis induced by thuja, another member of the Cupressaceae family. Monosensitization to thuja pollen has been identified as the causal agent: (1) of a long-standing springtime rhinitis in 1 patient with negative skin tests and specific IgE titers to the main inhalants (specificity of the prick test and nasal provocation with the thuja extract was confirmed by a positive RAST) and (2) in a 2nd patient without former history of allergy, who consulted for conjunctivitis following acquisition of a dog; the standard battery of skin tests, and Phadiotop were all negative. After controls, only the thuja extract gave significant skin test response. The level of total IgE was low, and RAST was negative. Sensitization and pollen provocation were produced by the intermediary of the dog, carrying thuja pollens on its fur. The immunoprint and the crossed radioimmunoelectrophoresis revealed common antigenicity between cypress and thuja extracts.
Subject(s)
Conjunctivitis, Allergic/etiology , Conjunctivitis, Allergic/immunology , Pollen/immunology , Rhinitis, Allergic, Seasonal/etiology , Rhinitis, Allergic, Seasonal/immunology , Adult , Aged , Animals , Dogs , Female , Humans , Intradermal Tests , Plant Extracts/chemistry , Plant Extracts/immunology , Pollen/chemistryABSTRACT
Fifty-two cases of perennial rhinitis were studied, leading to the diagnosis of seven cases of nonallergic rhinitis with eosinophilia syndrome (NARES) a frequency of 13.5%. Symptoms of nasal hyperreactivity involving sneezing, rhinorrhea, nasal obstruction and pruritus were more severe than in other types of rhinitis. The frequency of hyposmia was very specific to NARES. Nasal endoscopy and sinus CT revealed an evolution towards nasal polyposis in four patients. The nasal challenge to house dust mites showed the absence of any increase in local eosinophilia. Bronchial hyperreactivity to carbachol occurred in one case. There was no case of intolerance to aspirin. There was particular adrenergic hyperreactivity among the seven patients, evidenced by study of the reactivity of the cardiovascular alpha and beta receptors. The authors emphasize the features that are shared by NARES and by the triad, which suggest that NARES is the early phase of the triad. They advance the pathogenic hypothesis of an autonomic nervous system dysregulation with a predominating adrenergic hyperreactivity. Inflammatory effects of local release of neurotransmitters induce a switch from a neurogenic to a self-sustaining inflammation. Tissue eosinophilia is regulated by chemical attractants and activating substances of various origins and plays a major part in the chronic inflammatory state.
Subject(s)
Aspirin/pharmacology , Asthma/etiology , Eosinophilia/complications , Nasal Polyps/etiology , Rhinitis/complications , Adolescent , Adult , Child , Drug Tolerance , Female , Humans , Male , Middle Aged , SyndromeABSTRACT
Metapramine (Timaxel) was oxidised by hepatic microsomes from rat and by biomimetic chemical systems; vanadyl acetylacetonate-iodylbenzene, phthalocyanin-iron II-iodosylbenzene, meso tetraphenyl porphyrine-iron III chloride-iodosylbenzene and Fenton's reagent. The major metabolite in all cases was the monodemethylated product formed by oxydative removal of the methyl group on the endocyclic nitrogen atom.
Subject(s)
Antidepressive Agents, Tricyclic/metabolism , Dibenzazepines/metabolism , Microsomes, Liver/metabolism , Animals , Chromatography, Gas , Chromatography, High Pressure Liquid , Formaldehyde/metabolism , Hydrogen Peroxide , Iron , Male , Metalloporphyrins , Models, Chemical , Oxidation-Reduction , RatsABSTRACT
Gas chromatographic and mass spectrometric method is used to confirm the administration of 19-nortestosterone to cattle. The most abundant metabolites detected were 19-norepitestosterone and 5 alpha-oestrane-3 beta,17 alpha-diol.
Subject(s)
Cattle/metabolism , Nandrolone/metabolism , Animals , Cattle/urine , Estranes/analysis , Estranes/urine , Female , Gas Chromatography-Mass Spectrometry , Injections, Intramuscular , Nandrolone/administration & dosageABSTRACT
The main metabolite of flunixin, a hydroxylated product, has been identified by gas chromatography-mass spectrometry and 1H NMR spectroscopy in equine urine and plasma. The method also permits the qualitative monitoring of the urinary elimination of the drug and its metabolite. The two products are detected up to 175 and 54 h, respectively, after a single intravenous administration at the dose of 1 mg/kg. Simultaneous detection of the two compounds increases the reliability of anti-doping control analysis.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/analysis , Clonixin/analysis , Nicotinic Acids/analysis , Animals , Anti-Inflammatory Agents, Non-Steroidal/blood , Anti-Inflammatory Agents, Non-Steroidal/urine , Biotransformation , Clonixin/analogs & derivatives , Clonixin/blood , Clonixin/urine , Gas Chromatography-Mass Spectrometry , Horses , Magnetic Resonance Spectroscopy , Male , Spectrophotometry, InfraredABSTRACT
Chronopharmacokinetics of intravenous phenylbutazone in the horse was studied with the aim of antidoping control. Among parameters studied, the single one which seemed to depend on circadian rhythm was the elapsed time between the injection and the plasmatic peak. There was no relationship between the injection time and the both parameters: half-life and time required to reach the forensic level of 4 micrograms/ml. This later, and oxyphenbutazone/phenylbutazone ratio, should depend on individual factors. Therefore, the injection time should not be a main parameter for the phenylbutazone evaluation in the case of antidoping control.
