ABSTRACT
An animal model to study the fate of antinuclear antibodies (ANA) in vivo is reported. Newborn Balb/c mice were passively transferred by injection with sera from patients with autoimmune disease. The following antinuclear antibodies were found in the liver, spleen, kidneys and skin shortly after the injection: anti-dsDNA, nRNP, Ro/SSA and La/SSB. Clearance of all these antibodies occurred within 48 hours. The cytoskeleton, Fc immunoglobulin domain and Fc immunoglobulin-cell surface interaction may play a major role in the entry of antinuclear antibodies into cells, which may occur via endocytosis. Our animal model may be useful for studying the kinetics of binding of antinuclear antibodies to nuclear components and the effects of such binding in vivo.
Subject(s)
Antibodies, Anti-Idiotypic/metabolism , Antibodies, Antinuclear/metabolism , Immunization, Passive , Immunoglobulin G/immunology , Lupus Erythematosus, Systemic/immunology , Animals , Animals, Newborn , Binding Sites, Antibody , Disease Models, Animal , Fluorescent Antibody Technique, Indirect , Humans , Immunoglobulin G/metabolism , Lupus Erythematosus, Systemic/metabolism , Lupus Erythematosus, Systemic/pathology , Mice , Mice, Inbred BALB CABSTRACT
Pregnant female Balb/c mice were injected with IgG fractions from patients with systemic lupus erythematosus, in order to study the in vivo passage of antinuclear antibodies (ANA) across the placenta. After injection of monospecific sera directed against nDNA, Sm, nRNP, Ro(SSA) and La(SSB), ANA were found in fetal circulation and trapped in the liver, spleen kidney and skin of fetus. Also, ANA were demonstrated in placental tissue and cord. The placental IgG-Fc receptors apparently played a major role in ANA entry into the fetus. Our study demonstrates that human ANA can be passively transferred into experimental animals to study their kinetics during pregnancy.
Subject(s)
Antibodies, Antinuclear/pharmacokinetics , Maternal-Fetal Exchange , Placenta/metabolism , Animals , Female , Fetus/analysis , Humans , Immunoglobulin G/immunology , Injections , Lupus Erythematosus, Systemic/immunology , Mice , Mice, Inbred BALB C , Placenta/analysis , Pregnancy , Receptors, Fc/metabolismSubject(s)
Endocytosis/drug effects , Neutrophils/drug effects , Thalidomide/pharmacology , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/therapeutic use , Depression, Chemical , Humans , Leprosy/drug therapy , Leprosy/immunology , Neutrophils/physiology , Thalidomide/therapeutic useABSTRACT
Se demostraron anticuerpos anti-DNA nativo utilizando el cinetoplasto del parasito Crithidia luciliae como substrato. Se estudiaron 81 sueros, de los que 27 eran de pacientes con lupus eritematoso sistemico (LES), 20 de artritis reumatoide, 17 con padecimientos miscelaneos y 17 controles sanos. El 44% de los sueros de LES fueron positivos a la prueba y 11 de 14 sueros de pacientes con LES activo tuvieron titulos significativos de anticuerpos anti-DNA (p < 0.0005). Los grupos restantes fueron negativos. Esta prueba se considera util y sencilla para demostrar anticuerpos anti-DNA de doble cadena
