[Morphological characterization of experimental parodontitis during its treatment with a phyto-preparation uno de gato].

Examination of new methods of treatment of parodontitis is stipulated by the significant increase in the number of patients with parodontitis. Experiments were conducted on white outbred male rats weighing 150-200 gr, which were divided into three groups. The first group was a reference one (n=20). The second group includes induced parodontitis (n=15) and the third group includes induced parodontitis with the treatment of uno de gato (n=19). We simulated parodontitis in the following way: on the lower nippers in the field of a neck we attached a ligature causing mechanical damage. We used a intramuscular injection of Nembutal as a means of narcotization (0.1 ml-10 g per mass). The present work is dedicated to morphological studies of the structures of damaged parodontium during its treatment with a phyto-preparation uno de gato. The undertaken treatment has demonstrated partial restoration of the mucous tunic of the gum. Newly formed capillaries were found in the connective tissue. The fibroblasts are mostly mature and collagen-producing. Thus, the study has demonstrated that there is a certain tendency of the tissue restoration and the treatment has produced the best therapeutic results.

[Extra-islet cells of pancreas during the diabetes of alloxan and after the action of plaferon].

Using the methods of light and electron microscopy, pancreas was studied in the experimental model of alloxan diabetes (AD) at various times of the experiment (1 and 6 month) at different degrees of AD and after the action of plaferon. The special emphasis was made on display of compensatory changes that is opportunity of occurrence in extra-islet cells special granules, characteristic for insulin producing B-cells. During the development of pathological processes, there were detected intermediate cells including exocrine and endocrine granules. Some of these cells were located near the acini and some of them were in close contact with duct cells. It is suggested that these intermediate extra-islet cells are especial cells with high functional activity, they are transitional form during the processes of transformation into endocrine ones, possibly these cells are precursors of islet cells. It is possible, that plaferon acts on processes of transformation and differentiation of extra-islet cells and takes part in neogenesis of B-cells.

[Influence of connective tissue dysplasia on the clinical course of chronic catarrhal gingivitis].

"MASS"- phenotype or dysplasia of the connective tissue has been studied for many years, but it still remains the actual problem in clinical and theoretical medicine. Expansive integration of clinical genetics and molecular biology into the medicine has revealed the most complicated and multistage structure of the pathogenesis of this disease. The aim of our study was focused on the investigation of "MASS" phenotype and its influence on the stomatological disease such as the early stage of periodontitis -- chronic catarrhal gingivitis. Two groups of subjects with chronic catarrhal gingivitis were examined. Subjects in the first group had the chronic catarrhal gingivitis but did not belong to "MASS" phenotype, while the subjects in the second group had the chronic catarrhal gingivitis and belonged to "MASS" phenotype at the same time. Comparing clinical characteristics of these two groups, the clinical course of catarrhal gingivitis among subjects with "MASS" phenotype was characterised by more acute dynamics, weaker response to conventional treatment and more frequent recidives.

[Morphologic characteristics of microadenomas of the large intestine]. / Morfologicheskaia kharakteristika mikroadenom tolstoi kishki.

Tissue and cellular atypia, changes of the mucus chemical composition, protein secreting function, ATPase activity, differentiation of the epithelial cells and DNA replication with the widening of proliferation zone were registered morphologically in colon microadenomas. A pronounced increase of labelled small lymphocytes capable of proliferation is observed in the stroma. Fibrocytes are practically absent, fibroblasts are represented by young 3H-labelled forms. All these changes are considered as dysplastic.

[Hyperplastic changes in the mucosa in colon cancer]. / Giperplasticheskie izmeneniia v slizistoi obolochke pri rake tolstoi kishki.

67 segments of rectum and sigmoid removed surgically because of carcinoma were examined: the surface of intestinal mucosa stained with methylene-blue was studied in order to determine the number and localization of focal changes in the macroscopically unchanged areas. Colon microscopy was performed clinically in 24 patients with carcinoma of the rectum and in 24 control individuals. 28 hyperplastic polyps and 1037 microscopic hyperplastic foci were found in operative material. An active metabolism is established by means of electron autoradiography in the cells of hyperplastic focus. Areas of dysplastic epithelium were rarely found in big hyperplastic polyps. Areas of diffuse hyperplasia (transitional mucosa) with crypt deepening and presence of a high number of goblet cells were found near tumour. A certain role of hyperplastic processes in carcinogenesis is forwarded. Numerous microscopic changes in the mucosa epithelium may testify against the carcinoma development de novo.

[The parenchymatous-stromal interrelationships in adenocarcinomas of the large intestine (an electron radioautographic study)]. / Parenkhimatozno-stromal'nye vzaimootnosheniia v adenokartsinomakh tolstoi kishki (élektronno-radioavtograficheskoe issledovanie).

Parenchymatous and stromal cells in colonic adenocarcinomas were studied electron-radiographically. The properties of the nucleic acid and protein synthesis in the cylindrical and goblet cells in carcinoma (partial 3H-thymidine incorporation, heterogeneity of 3H-uridine and amino acid mixture incorporation) indicate a relatively low level of their differentiation, lower degree of their participation in a specific function, retaining their capacity to proliferate. A high number of 3H-thymidine-labelled stem cells which are the main source of tumour growth is observed in carcinoma. Stroma in carcinoma is characterized by endotheliocyte and fibroblast proliferation (3H-thymidine incorporation), high degree of metabolic processes in some of these cells (3H-uridine and amino acid mixture incorporation) and by the disturbance of immunocompetent cell specific function (partial and weak labelling of the immunocompetent cells with 3H-uridine and amino acid mixture).

[Cloacogenic cancer of the rectum]. / Kloakogennyi rak lriamoi Kishki.

Three cases of cloacogenous and 35 cases of squamous-cell carcinoma of the distal part of the rectum and anus were studied and compared histochemically to reveal considerable differences in mucopolysaccharides contained by the tumours. Squamous-cell carcinomas were characterized by the presence of glycogen which was not detected in cloacogenous carcinomas. In squamous-cell carcinomas, glycosamine glycanes were represented by sulfo- and sialo-mucin, while cloacogenous carcinomas largely contained a non-sulfated mucosubstance. The evidence obtained allows one to distinguish, among anal carcinomas of the rectum, a group of cloacogenous carcinomas that arose from the transitional epithelium of the serrate line and that differ from squamous-cell carcinomas in specific metabolic processes.