ABSTRACT
The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A Methanosarcina sp. dominated the thermophilic, while a Methanosaeta sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate reducing respiratory bacteria could be detected. The PLFA profiles of the digester communities were similar though in minor components characteristic differences were shown. Level of branched chain fatty acids is slightly lower in the thermophilic digester that reports less Gram positive bacteria. The relative ratio of fatty acids characteristic to Enterobacteriaceae, Bacteroidetes and Clostridia shows differences between the two digesters: their importance generally decreased under thermophilic conditions. The sulphate reducer marker (15:1 and 17:1) fatty acids are present in low quantity in both digesters.
Subject(s)
Archaea/classification , Archaea/growth & development , Bacteria/classification , Bacteria/growth & development , Biodiversity , Sewage/microbiology , Anaerobiosis , Archaea/chemistry , Archaea/genetics , Archaea/isolation & purification , Bacteria/chemistry , Bacteria/genetics , Bacteria/isolation & purification , Bacteroidetes/isolation & purification , DNA Fingerprinting , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Enterobacteriaceae/isolation & purification , Fatty Acids/analysis , Gram-Positive Bacteria/isolation & purification , Hot Temperature , Methanosarcina/isolation & purification , Methanosarcinales/isolation & purification , Phospholipids/analysis , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Quinones/analysis , Sequence Analysis, DNAABSTRACT
The cause of nosocomial infections and their possible solutions, according to the chemical compounds of rubber and plastic equipments are shortly summarized. Compounds of rubbers and plastics act as culture medium for bacteria and fungi being causative agents in nosocomial infections and because of their long-term persistence they can be source of such infections. This problem needs cooperation between experts and specialists from different scientific fields working in both industry and medicine. Only "united forces" can make steps in order to find solutions to these emerging questions and problems.
Subject(s)
Candida albicans/growth & development , Candida/growth & development , Equipment and Supplies , Pseudomonas aeruginosa/growth & development , Pseudomonas/growth & development , Corrosion , Equipment Contamination/prevention & control , Quality ControlABSTRACT
In the course of the study the photopolimerizating filling materials (Visiomolar, Heliomolar, Heliosit, Silux, P-50, Prismatic) and the glass ionomer cements (Ketacfil, Ketac-silver) served as supposed sources of carbon for Ps. aeruginosa. All the examined materials have shown a suitable resistance to the studied bacterium species. If sunflower oil has been absorbed onto the surface of the examined materials, then a limited increase of the living germ counts has been observed. Ketac-silver was proved to be bactericidal in every case.
Subject(s)
Dental Materials/chemistry , Dental Restoration, Permanent , Drug Resistance, Microbial , Glass Ionomer Cements/standards , Composite Resins , Humans , PolymersABSTRACT
Inertia in labour could be more often observed and the number of intra-uterine foetal distress signs increased in case of bacterial infection of the maternal uropoietic and urinary system. Consequently, stimulation of labour pains and surgical termination of the delivery were required several times. The data in concert with those mentioned in the first part of the study indicate that the clinical pictures associated with the bacterial infections of the urine constitute a significant part of the pathological aspects of pregnancy.
Subject(s)
Bacterial Infections/complications , Obstetric Labor Complications , Pregnancy Complications, Infectious , Urinary Tract Infections/complications , Female , Fetal Distress/etiology , Humans , Pregnancy , Risk , Time Factors , Uterine Inertia/etiologyABSTRACT
The resistance to bacteria of composition filling materials (Evicrol, Isopast, Micromix and Superlux), of acrylic base-plate used for removable dentures and of acrylic (Medident) teeth has been examined. Out of the 6 samples tested with Pseudomonas aeruginosa in accordance with the Hungarian standard, Evicrol, Superlux and the base-plate proved to be resistant; Isopast was attacked moderately and Medident teeth and Micromix showed intermediate results.
Subject(s)
Acrylic Resins , Composite Resins , Dentures , Methylmethacrylates , Pseudomonas aeruginosa/growth & development , Tooth, Artificial , Bacteriological Techniques , Culture Media , PolyurethanesSubject(s)
Dental Care , Dental Clinics , Community Dentistry , Delivery of Health Care , Hungary , Rural PopulationABSTRACT
The qualitative and quantitative changes in the bacterial flora of the duodenal juice after truncal vagotomy have been studied in self-control experiments in dogs. The intervention was followed by a rise in the bacterial count without a change in qualitative composition. It is suggested that the accumulation of microorganisms might contribute to the development of postoperative diarrhoea. The role of several partly clarified pathophysiological processes are pointed out.
