ABSTRACT
OBJECTIVE: The efficacy of two-drug combination treatment may be schedule-dependent. We investigated a simulated in-vitro interaction between taxol and doxorubicin in a Cervical cancer cell line HeLa and the role of peroxiredoxin in cytotoxicity. METHODS: Two contradicting schedules of two drugs (taxol followed by doxorubicin or vice versa) were compared each other in terms of cytotoxicity in parental HeLa cell line and the peroxiredoxin (prx)-overexpressing variant. Cytotoxic activity was determined by MTT assay. Cell cycle pertubation was evaluated by flow cytometric analysis. Protein levels were determined by western blot. RESULTS: The sequential treatment of taxol followed by doxorubicin (T--Subject(s)
Humans
, Appointments and Schedules
, Blotting, Western
, Cell Cycle
, Cell Line
, Cell Line, Tumor
, Cyclin D1
, Doxorubicin
, HeLa Cells
, Paclitaxel
, Parents
, Peroxiredoxins
, Uterine Cervical Neoplasms
