3D T1 relaxation time measurements in an equine model of subtle post-traumatic osteoarthritis using MB-SWIFT.

The aim of this study is to assess whether articular cartilage changes in an equine model of post-traumatic osteoarthritis (PTOA), induced by surgical creation of standard (blunt) grooves, and very subtle sharp grooves, could be detected with ex vivo T1 relaxation time mapping utilizing three-dimensional (3D) readout sequence with zero echo time. Grooves were made on the articular surfaces of the middle carpal and radiocarpal joints of nine mature Shetland ponies and osteochondral samples were harvested at 39 weeks after being euthanized under respective ethical permissions. T1 relaxation times of the samples (n = 8 + 8 for experimental and n = 12 for contralateral controls) were measured with a variable flip angle 3D multiband-sweep imaging with Fourier transform sequence. Equilibrium and instantaneous Young's moduli and proteoglycan (PG) content from OD of Safranin-O-stained histological sections were measured and utilized as reference parameters for the T1 relaxation times. T1 relaxation time was significantly (p < 0.05) increased in both groove areas, particularly in the blunt grooves, compared with control samples, with the largest changes observed in the superficial half of the cartilage. T1 relaxation times correlated weakly (Rs ≈ 0.33) with equilibrium modulus and PG content (Rs ≈ 0.21). T1 relaxation time in the superficial articular cartilage is sensitive to changes induced by the blunt grooves but not to the much subtler sharp grooves, at the 39-week timepoint post-injury. These findings support that T1 relaxation time has potential in detection of mild PTOA, albeit the most subtle changes could not be detected.

T2orientation anisotropy mapping of articular cartilage using qMRI.

Objective.To provide orientation-independent MR parameters potentially sensitive to articular cartilage degeneration by measuring isotropic and anisotropic components ofT2relaxation, as well as 3D fiber orientation angle and anisotropy via multi-orientation MR scans.Approach. Seven bovine osteochondral plugs were scanned with a high angular resolution of thirty-seven orientations spanning 180° at 9.4 T. The obtained data was fitted to the magic angle model of anisotropicT2relaxation to produce pixel-wise maps of the parameters of interest. Quantitative Polarized Light Microscopy (qPLM) was used as a reference method for the anisotropy and fiber orientation.Main results. The number of scanned orientations was found to be sufficient for estimating both fiber orientation and anisotropy maps. The relaxation anisotropy maps demonstrated a high correspondence with qPLM reference measurements of the collagen anisotropy of the samples. The scans also enabled calculating orientation-independentT2maps. Little spatial variation was observed in the isotropic component ofT2while the anisotropic component was much faster in the deep radial zone of cartilage. The estimated fiber orientation spanned the expected 0°-90° in samples that had a sufficiently thick superficial layer. The orientation-independent magnetic resonance imaging (MRI) measures can potentially reflect the true properties of articular cartilage more precisely and robustly.Significance. The methods presented in this study will likely improve the specificity of cartilage qMRI by allowing the assessment of the physical properties such as orientation and anisotropy of collagen fibers in articular cartilage.

New methods for robust continuous wave T1ρ relaxation preparation.

Measurement of the longitudinal relaxation time in the rotating frame of reference (T1ρ ) is sensitive to the fidelity of the main imaging magnetic field (B0 ) and that of the RF pulse (B1 ). The purpose of this study was to introduce methods for producing continuous wave (CW) T1ρ contrast with improved robustness against field inhomogeneities and to compare the sensitivities of several existing and the novel T1ρ contrast generation methods with the B0 and B1 field inhomogeneities. Four hard-pulse and four adiabatic CW-T1ρ magnetization preparations were investigated. Bloch simulations and experimental measurements at different spin-lock amplitudes under ideal and non-ideal conditions, as well as theoretical analysis of the hard-pulse preparations, were conducted to assess the sensitivity of the methods to field inhomogeneities, at low (ω1 << ΔB0 ) and high (ω1 >> ΔB0 ) spin-locking field strengths. In simulations, previously reported single-refocus and new triple-refocus hard-pulse and double-refocus adiabatic preparation schemes were found to be the most robust. The mean normalized absolute deviation between the experimentally measured relaxation times under ideal and non-ideal conditions was found to be smallest for the refocused preparation schemes and broadly in agreement with the sensitivities observed in simulations. Experimentally, all refocused preparations performed better than those that were non-refocused. The findings promote the use of the previously reported hard-pulse single-refocus ΔB0 and B1 insensitive T1ρ as a robust method with minimal RF energy deposition. The double-refocus adiabatic B1 insensitive rotation-4 CW-T1ρ preparation offers further improved insensitivity to field variations, but because of the extra RF deposition, may be preferred for ex vivo applications.

Harmonization of technical image quality in computed tomography: comparison between different reconstruction algorithms and kernels from six scanners.

Purpose. The radiology department faces a large number of reconstruction algorithms and kernels during their computed tomography (CT) optimization process. These reconstruction methods are proprietary and ensuring consistent image quality between scanners is becoming increasingly difficult. This study contributes to solving this challenge in CT image quality harmonization by modifying and evaluating a reconstruction algorithm and kernel matching scheme.Methods. The Catphan 600 phantom was scanned with six different CT scanners from four vendors. The phantom was scanned with volumetric CT dose indices (CTDIvols) of 10 mGy and 40 mGy, and the data were reconstructed using 1 mm and 5 mm slices with each combination of reconstruction algorithm, body region kernel, and iterative and deep learning reconstruction strength. A matching scheme developed in previous research, which utilizes the noise power spectrum (NPS) and modulation transfer function (MTF), was modified based on our organization's needs and used to identify the matching reconstruction algorithms and kernels between different scanners.Results. The matching paradigm produced good matching results, and the mean ± standard deviation (median) matching function values for the different acquisition settings were (a value of 1 indicates a perfect match): CTDIvol 10 mGy, 1 mm slice: 0.78 ± 0.31 (0.94); CTDIvol 10 mGy, 5 mm slice: 0.75 ± 0.33 (0.93); CTDIvol 40 mGy, 1 mm slice: 0.81 ± 0.28 (0.95); CTDIvol 40 mGy, 5 mm slice: 0.75 ± 0.33 (0.93). In general, soft reconstruction kernels, i.e., noise-reducing kernels that reduce sharpness, of one vendor were matched with the soft kernels of another vendor, and vice versa for sharper kernels. Conclusions. Combined quantitative assessment of NPS and MTF allows effective strategy for harmonization of technical image quality between different CT scanners. A software was also shared to support CT image quality harmonization in other institutions.