Gathering the evidence: health and aged care carbon inventory study.

Objective This study aimed to calculate the baseline carbon inventory of Mercy Health, a provider of health and aged care services in Australia, across emission Scopes 1, 2 and 3. The carbon inventory has clarified the baseline environmental impact, identified carbon hotspots and will inform emissions reduction interventions and a decarbonisation trajectory. Methods A hybrid carbon footprinting methodology was devised. Established carbon footprinting standards provided methodological guidance. A consulting firm with health service carbon accounting experience provided expertise, rigour and objectivity to the work. Results In the 2020-21 financial year, the carbon footprint of Mercy Health was 102.96k tCO2-e. Scope 1 emissions accounted for 11.07% (11.40k tCO2-e), followed by Scope 2 with 29.80% (30.68k tCO2-e) and Scope 3 with 59.13% (60.88k tCO2-e). The largest carbon impost group was Building energy (42.01%; 43.25k tCO2-e), followed by Food and catering (9.42%; 9.70k tCO2-e) and Business services (7.74%; 7.97k tCO2-e). Mercy Health's Health Services, Aged Care and Support Services divisions contributed 49.16, 47.81 and 3.03% (50.61k, 49.23k and 3.12k tCO2-e) of total greenhouse gas emissions respectively. Conclusions Mercy Health's Health Services division and Aged Care division each comprised around half of total organisation carbon emissions. Building energy dominated emissions, particularly electricity. The study discovered meaningful differences in the composition of carbon emissions in operational divisions of the organisation, indicating tailored interventions will be required to meet carbon abatement targets. The study demonstrates the benefit of conducting carbon footprinting within individual organisations, and the importance of studies within the Australian context.

Exploring the influence of natural cosolvents on the free energy and conformational landscape of filamentous actin and microtubules.

Actin and tubulin, the main components of the cytoskeleton, are responsible for many different cellular functions and can be found in nearly all eukaryotic cells. The formation of filamentous actin (F-actin) as well as microtubules depends strongly on environmental and solution conditions. The self-assembly of both, actin and tubulin, has been found to be among the most pressure sensitive process in vivo. Here, we explored the effects of various types of natural cosolvents, such as urea and the osmolyte trimethylamine-N-oxide (TMAO), on the temperature- and pressure-dependent stability of their polymeric states, F-actin and microtubules. Accumulation of TMAO by deep-sea animals is proposed to protect against destabilizing effects of pressure. The pressure and temperature of unfolding as well as associated enthalpy and volume changes have been determined using Fourier-transform infrared spectroscopy, covering a wide range of pressures and temperatures, ranging from 1 bar to 11 kbar and from 20 to 90 °C, respectively. Complementary thermodynamic measurements have been carried out using differential scanning and pressure perturbation calorimetry. The results obtained helped us explore the effect of the cellular milieu on the limitations of the pressure stability of cytoskeletal assemblies. Conversely to urea, the pressure stability of both polymers increases dramatically in the presence of TMAO, counteracting detrimental effects of both, urea and pressure.