ABSTRACT
Because of the increasing interest on stimulating fracture healing, knowledge about the role and chronology of growth factors during the healing process is important. The purpose of this study was to quantify the protein concentration of IGF-I and TGF-beta1 during rat tibial fracture healing 5, 10, and 15 days after fracture using ELISA methods and to analyze the distribution of the proteins and the related mRNA expression in the fracture callus by immunohistochemistry and in situ hybridization. The following three groups were analyzed: Fractured tibiae intramedullary stabilized with K-wires coated with IGF-I and TGF-beta1 compared with fractures stabilized with uncoated K-wires and unfractured tibiae. The weight of the callus increased during the healing process in both experimental groups. The protein concentration of IGF-I and TGF-beta1 in the fracture callus showed significant changes between the investigated time points and treatment groups compared with the unfractured tibia. IGF-I increased with healing time whereas TGF-beta1 revealed a constantly elevated level at the investigated time points. Mesenchymal cells, osteoblasts, osteocytes, proliferating and immature chondrocytes, and osteoclasts expressed both growth factors. No differences in the expression and localization pattern of the growth factors were detectable among the groups. Using the different methods for quantification and visualization of the growth factors, no differences (except the increased IGF-I concentration at day 15 in the growth factor group) were seen between the normal and the growth factor-stimulated fracture healing as an indication for physiological healing after exogenous growth factor treatment.
Subject(s)
Fracture Healing/drug effects , Insulin-Like Growth Factor I/pharmacology , Tibial Fractures/metabolism , Transforming Growth Factor beta/pharmacology , Absorbable Implants , Animals , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Female , Fracture Healing/physiology , Humans , Immunoenzyme Techniques , In Situ Hybridization , Insulin-Like Growth Factor I/genetics , Insulin-Like Growth Factor I/metabolism , Polyesters/administration & dosage , Polymers , Proteins/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Transforming Growth Factor beta/genetics , Transforming Growth Factor beta/metabolism , Transforming Growth Factor beta1ABSTRACT
Experimental results are presented from vacuum-ultraviolet free-electron laser (FEL) operating in the self-amplified spontaneous emission (SASE) mode. The generation of ultrashort radiation pulses became possible due to specific tailoring of the bunch charge distribution. A complete characterization of the linear and nonlinear modes of the SASE FEL operation was performed. At saturation the FEL produces ultrashort pulses (30-100 fs FWHM) with a peak radiation power in the GW level and with full transverse coherence. The wavelength was tuned in the range of 95-105 nm.
ABSTRACT
An experiment has been carried out at the TESLA Test Facility linac to investigate the wake fields generated by picosecond electron bunches in narrow beam pipes with an artificially roughened inner surface. The energy structure imposed on the bunches by the wake fields has been analyzed with a magnetic spectrometer. Strong harmonic-wake-field effects are observed as expected from simulations in which the rough surface is modeled by a dielectric layer.
