ABSTRACT
OBJECTIVE: To examine the efficacy of a strength-focused mutual support group for reducing stress and enhancing psychological well-being of the caretakers of children with cerebral palsy. METHODS: This pre- and post-intervention outcome study, conducted by Department of Orthopaedics and Traumatology, Duchess of Kent Children's Hospital, recruited 12 primary caretakers of children with cerebral palsy in Hong Kong. A strength-focused support group manual was developed to help such caretakers to identify and cultivate the character strengths of their children and enhance their own positive emotions. Participants were asked to complete a full set of questionnaires at 3 time-points: before and immediately after the intervention (consisting of 4 weekly sessions), and after the 1-month follow-up booster session. Two additional questionnaires were administered before each session to check mood. Parenting stress, anxiety, depression, social support, hope, and other psychological well-being measures were also assessed. RESULTS: Half of the caretakers (n = 6) who had attended the full intervention programme were included in the data analysis. Participants exhibited a significantly lower level of parental stress and higher hope level both after the 4 intervention sessions and at the booster session. Their perceived social support was significantly increased when the group was ongoing but not after it ended. CONCLUSION: This group intervention programme could effectively help caretakers reduce their parenting stress and enhance their hopefulness. Launching a similar programme with more sessions and regular follow-up sessions might help maintain positive effects and establish a social support network.
Subject(s)
Caregivers/psychology , Cerebral Palsy/therapy , Self-Help Groups/statistics & numerical data , Adaptation, Psychological , Adult , Cerebral Palsy/psychology , Emotions , Female , Follow-Up Studies , Hong Kong , Humans , Male , Middle Aged , Parents/psychology , Personal Satisfaction , Program Evaluation/methods , Program Evaluation/statistics & numerical data , Social Support , Stress, Psychological/psychology , Stress, Psychological/therapy , Surveys and QuestionnairesABSTRACT
The processing pathway of G-proteins and Ras family proteins includes the isoprenylation of the cysteine residue, followed by proteolysis of three terminal residues and alpha-carboxyl methyl esterification of the cysteine residue. Farnesylcysteine methyltransferase (FCMT) activity is responsible for the methylation reaction which play a role in the membrane attachment of a variety of cellular proteins. Four kinds of Ras protein (c-Ha-ras, c-N-Ras, c-Ki-Ras, pan-Ras) expression were detected in adenocarcinoma of human tissue by immunohistochemical method, and hematoxylin and eosin staining. The level of Ras protein in human stomach tumor tissues was much higher than in normal and peritumoral regions of the same biopsy samples. The FCMT activities of each cellular fractions were high in mitochondrial fraction followed by microsomal fraction, whole homogenate and cytosolic fraction. The inhibitory effect on FCMT activity on stomach tumor tissue was determined after treatment with 0.25 microM of S-adenosyl-L-homocysteine. S-adenosyl-L-homocysteine inhibited FCMT activity from 11.2% to 30.5%. These results suggested that FCMT might be involved in Ras proteins activity.
Subject(s)
Cysteine/analogs & derivatives , Protein Methyltransferases/metabolism , Stomach Neoplasms/enzymology , ras Proteins/biosynthesis , Adenocarcinoma/enzymology , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Adult , Aged , Cysteine/metabolism , Enzyme Activation/drug effects , Gene Expression , Humans , Immunohistochemistry , Middle Aged , Protein Methyltransferases/antagonists & inhibitors , S-Adenosylhomocysteine/pharmacology , Stomach/enzymology , Stomach Neoplasms/metabolism , Stomach Neoplasms/pathology , ras Proteins/analysis , ras Proteins/metabolismABSTRACT
In the self-fertilizing hermaphroditic fish, Rivulus marmoratus, the susceptibility to tumor induction by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was evaluated. Seven-day-old fish larvae were exposed for 2 h to MNNG at concentrations ranging from 5 to 25 ppm in a static water bath. The exposed fish were observed at 2 and 4 months after carcinogen treatment to assess tumor development. Within 4 months after 25 ppm MNNG exposure, nearly all fish developed thyroid tumors. The tumor incidences were dose- and time-dependent, and the latent period of tumor induction was less than 2 months. Most induced neoplasms were papillary carcinomas similar histologically to those of rodents and humans, and the tumors were serially transplantable to other fish of the same species. These results demonstrate that rivulus could be useful as a model of thyroid carcinogenesis.
