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2.
Inflamm Res ; 55(7): 293-9, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16955392

ABSTRACT

OBJECTIVE: Synovial sarcoma (SS) is a malignant mesenchymal tumor that accounts for 5-10% of all soft tissue sarcoma. IL-1beta, a pleiotrophic cytokine, has been found in the tumor microenvironment which plays crucial roles in the pathogenesis of tumors. METHODS: In this study, we used Hs701.T as a cellular model to study the short-term (4-h) and long-term (48-h) stimulatory effect of IL-1beta on cell proliferation and differential gene expression. RESULTS: The results showed that IL-1beta can stimulate cell proliferation through activation of NF-kappaB and AP-1 transcription factors; sequentially triggers the expression of genes related to tumor progression. The microarray data indicated that most of the up-regulated genes were related to tumor progression. Five candidate genes which are involved in the mediation of proliferation (IL-6), apoptosis (Hsp27 and Daxx), and angiogenesis (PlGF and SPARC) were further validated by RT-PCR. CONCLUSION: These findings may be useful for understanding the pathogenesis of synovial sarcoma.


Subject(s)
Gene Expression Profiling , Gene Expression Regulation, Neoplastic , Interleukin-1beta/metabolism , Sarcoma, Synovial/metabolism , Synovial Membrane/pathology , Cell Line, Tumor , Cell Nucleus/metabolism , Cell Proliferation , Disease Progression , Fibroblasts/metabolism , Humans , Neoplasms/blood supply , Neovascularization, Pathologic , Oligonucleotide Array Sequence Analysis , Time Factors
3.
Life Sci ; 79(7): 665-73, 2006 Jul 10.
Article in English | MEDLINE | ID: mdl-16566946

ABSTRACT

Sinomenine is an alkaloid with pharmacological effects of anti-inflammation, anti-angiogenesis, anti-arthritis and immunosuppression. This study aimed to investigate the effect of sinomenine on gene expression of human synovial sarcoma cells (Hs701.T) activated by IL-1 beta. The proliferative effect of sinomenine was examined in the presence or absence of IL-1 beta by the [3H]-thymidine incorporation and MTT assay, respectively. Using DNA microarray technology and RT-PCR, the activating action of IL-1 beta and modulatory effect of sinomenine on Hs701.T were simultaneously determined. Results showed that IL-1 beta could stimulate the proliferation and gene expression of Hs701.T cells. Sinomenine could significantly inhibit proliferation of IL-1 beta-activated Hs701.T cells and suppress expression of 17 genes including IL-6, PlGF, Daxx, and HSP27. These genes were found to be important in tumor progression through the mediation of inflammation, cell adhesion, proliferation, apoptosis and angiogenesis. In conclusion, our study provides supplementary information for the further studies on the pharmacological effects of sinomenine acting on synovial sarcoma.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Interleukin-1/biosynthesis , Interleukin-1/genetics , Morphinans/pharmacology , Sarcoma/metabolism , Synovial Membrane/metabolism , Cell Division/drug effects , Cell Line, Tumor , Cell Survival/drug effects , DNA, Neoplasm/biosynthesis , Gene Expression/drug effects , Gene Expression Profiling , Humans , Oligonucleotide Array Sequence Analysis , Reverse Transcriptase Polymerase Chain Reaction
4.
Angiogenesis ; 8(3): 205-16, 2005.
Article in English | MEDLINE | ID: mdl-16328162

ABSTRACT

The major active constituents of ginseng are ginsenosides, and Rg(1) is a predominant compound of the total extract. Recent studies have demonstrated that Rg(1) can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg(1). We report that Rg(1) induces the proliferation of HUVECs, monitored using [(3)H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg(1) (150-600 nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg(1) was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg(1) revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg(1) can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell-cell adhesion and migration.


Subject(s)
Gene Expression Regulation/drug effects , Ginsenosides/metabolism , Neovascularization, Physiologic/physiology , Panax/chemistry , Cell Adhesion/drug effects , Cell Line , Collagen , Cytoskeletal Proteins/metabolism , DNA Primers , Drug Combinations , Ginsenosides/pharmacology , Humans , Laminin , Models, Biological , Neovascularization, Physiologic/drug effects , Oligonucleotide Array Sequence Analysis , Plant Extracts/metabolism , Plant Extracts/pharmacology , Proteoglycans , Reverse Transcriptase Polymerase Chain Reaction , Thymidine/metabolism , Tritium
5.
Br J Nutr ; 93(1): 21-9, 2005 Jan.
Article in English | MEDLINE | ID: mdl-15705221

ABSTRACT

Si-Jun-Zi decoction (SJZD), a traditional Chinese herbal prescription, has been used clinically for treating patients with disorders of the digestive system. Previous studies indicated that the polysaccharides of SJZD (SJZPS) are the active components contributing towards its pharmacological effects in improving gastrointestinal function and immunity. However, the protective and restitutive effects on intestinal epithelial cells remain unknown. In the present study, SJZPS were first extracted and chemically characterized. Then their stimulatory and restitutive effects on intestinal epithelial cells (IEC-6 cells) were elicited by different in vitro models including migration of wounded IEC-6 cells and cell proliferation. Results indicated that SJZPS not only protects the cells against the harmful impairment of indomethacin but also enhances re-epithelialization of a wounded monolayer at an optimal dose of 100 mug/ml at 24 h incubation. To elucidate the modulatory effect of SJZPS on wounded IEC-6 cells at the molecular level, an oligonucleotide microarray was employed to study differential gene expression of SJZPS-treated IEC-6 cells and the candidate genes were validated by RT-PCR. There was increased expression of genes coding for ion channels and transporters, which are critical to cell migration and restoration of wounded intestinal cells, suggesting a possible mechanism for re-epithelialization. In conclusion, our data show for the first time that SJZPS can enhance intestinal restitution and protect against indomethacin-induced damage of intestinal epithelial cells. These findings provide new insight into the mechanism of action of a traditional Chinese herbal prescription, SJZD, in intestinal wound restitution.


Subject(s)
Drugs, Chinese Herbal/pharmacology , Intestinal Mucosa/drug effects , Polysaccharides/pharmacology , Wound Healing/drug effects , Animals , Cell Line , Cell Movement/drug effects , Dose-Response Relationship, Drug , Drugs, Chinese Herbal/chemistry , Gene Expression Profiling/methods , Gene Expression Regulation/drug effects , Intestinal Mucosa/metabolism , Intestinal Mucosa/pathology , Polysaccharides/chemistry , Rats , Reverse Transcriptase Polymerase Chain Reaction/methods , Wound Healing/physiology
6.
Life Sci ; 75(26): 3157-71, 2004 Nov 12.
Article in English | MEDLINE | ID: mdl-15488895

ABSTRACT

Homocysteine (Hcy) is a by-product of methionine metabolism. An imbalance of Hcy in the body may lead to hyperhomocysteinemia, a condition with elevated Hcy concentration in blood that may be one of the risk factors responsible for the development of several vascular diseases (thromboembolism, atherosclerosis, stroke, vascular diseases and dementia). Radix Salvia miltiorrhiza (Danshen), a well-known Chinese medicinal herb that can activate and improve blood microcirculation, is noticeable for its beneficial effect in treating cardiovascular diseases. The present study is to demonstrate the protective effect of Danshen extract against the homocysteine-induced adverse effect on human umbilical vein endothelial cell (HUVEC). Homocysteine (5 mM) not only decreased the cell viability but also caused the disruption of capillary-like structure formation in vitro. The protective effect of Danshen aqueous extract and its active compounds on endothelial cell function were demonstrated through an in vitro tube formation assay, which mimics the new blood vessel formation. To identify the active components in the aqueous extract of Danshen, the content was characterized by instrumental analysis using high performance liquid chromatography with diode array detector (DAD) and electrospray tandem mass spectrometry (ESI-MS/MS). Interestingly, Danshen extract and its pure compounds showed different effectiveness in protecting HUVEC against Hcy-induced injury according to the following descending order: Danshen aqueous extract, 3-(3,4-dihydroxy-phenyl)-2-hydroxy-propionic acid (Danshensu), protocatechuic acid, catechin and protocatechualdehyde. We believed that such findings might provide evidence in understanding the beneficial effects of Danshen on the cardiovascular system.


Subject(s)
Drugs, Chinese Herbal/pharmacology , Endothelial Cells/drug effects , Homocysteine/toxicity , Lactates/pharmacology , Salvia miltiorrhiza/chemistry , Benzaldehydes/pharmacology , Catechin/pharmacology , Catechols/pharmacology , Cell Survival/drug effects , Chromatography, High Pressure Liquid , Dose-Response Relationship, Drug , Drugs, Chinese Herbal/isolation & purification , Humans , Hydroxybenzoates/pharmacology , Lactates/isolation & purification , Spectrometry, Mass, Electrospray Ionization , Umbilical Veins/drug effects
7.
J Agric Food Chem ; 50(7): 1871-5, 2002 Mar 27.
Article in English | MEDLINE | ID: mdl-11902926

ABSTRACT

AFLP profiles characteristic to Panax ginseng and Panax quinquefolius were generated using primers E-AGG/M-CAA. P. ginseng samples from different farms in China and Korea are homogeneous genetically [similarity index (SI) = 0.88-0.99], whereas samples of P. quinquefolius from different sources are much more heterogeneous (SI = 0.64-0.96). Detailed analysis of one of the polymorphic bands in P. ginseng led to the identification of a minisatellite Pg2, which contains eight repeats of 5'-AGGACTCATCACATTGTTACTC. The minisatellite DNA was consequently used in directed amplification minisatellite region DNA analysis to authenticate the two ginsengs.


Subject(s)
DNA, Plant/analysis , Gene Amplification , Minisatellite Repeats , Panax/genetics , Polymorphism, Genetic , Base Sequence , DNA Fingerprinting , DNA Primers , Drugs, Chinese Herbal , Molecular Sequence Data , Polymerase Chain Reaction , Quality Control
8.
Planta Med ; 67(8): 781-3, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11731932

ABSTRACT

A 420-bp RAPD fragment from Panax quinquefolius was converted to a sequence characterized amplified region (SCAR) marker. The main difference between the SCAR of P. quinquefolius and its homolog in P. ginseng is the presence of a 25 bp insertion in the latter. Primers derived from this sequence were successfully used to authenticate six Panax species and two common adulterants.


Subject(s)
Panax/genetics , Random Amplified Polymorphic DNA Technique/methods , Base Sequence , DNA Primers , DNA, Plant , Drug Contamination , Drugs, Chinese Herbal , Molecular Sequence Data , Panax/classification , Plant Roots , Sequence Alignment
9.
Planta Med ; 67(6): 587-9, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11509992

ABSTRACT

The method of direct amplification of length polymorphism (DALP) was applied to authenticate Panax ginseng and P. quinquefolius. A 636-bp DALP fragment was present in all P. ginseng but absent in all the P. quinquefolius cultivars examined. We have shown that the use of DALP and conversion of specific polymorphic band to sequence-tagged site (STS) for quick authentication may be applied to authenticate related medicinal materials.


Subject(s)
Panax/classification , Polymorphism, Genetic , Base Sequence , DNA, Plant , Drugs, Chinese Herbal , Electrophoresis, Polyacrylamide Gel , Molecular Sequence Data , Nucleic Acid Amplification Techniques , Panax/genetics , Plant Extracts/genetics , Plant Roots/genetics , Sequence Tagged Sites , Species Specificity
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