ABSTRACT
Peptide immunohistochemistry (IHC) controls are a new quality control format for verifying proper IHC assay performance, offering advantages in high throughput automated manufacture and standardization. We previously demonstrated that formalin-fixed peptide epitopes, covalently attached to glass microscope slides, behaved (immunochemically) in a similar fashion to the native protein in tissue sections. To convert this promising idea into a practical clinical laboratory quality control tool, we tested the hypothesis that the quality assurance information provided by peptide IHC controls accurately reflects IHC staining performance among a diverse group of clinical laboratories. To test the hypothesis, we first designed and built an instrument for reproducibly printing the controls on microscope slides and a simple software program to measure the color intensity of stained controls. Automated printing of peptide spots was reproducible, with coefficients of variation of 4% to 8%. Moreover, the peptide controls were stable at Subject(s)
Epitopes/analysis
, Immunohistochemistry/standards
, Peptides/analysis
, Staining and Labeling/standards
, Breast Neoplasms/diagnosis
, Cell Line, Tumor
, Epitopes/immunology
, Humans
, Immunohistochemistry/instrumentation
, Peptides/immunology
, Quality Control
, Receptor, ErbB-2/analysis
, Receptor, ErbB-2/immunology
, Reference Standards
, Software
ABSTRACT
Experimental trials were conducted at Integrated Pest Management Programme, National Agriculture Research Centre Islamabad, to evaluate the resistance of host plants (cereals) against Rhopalosiphum padi (L.). For evaluation of susceptibility, twenty varieties/-advanced lines of National Uniform Wheat Yield Trails (NUWYT) Normal (N) of year 2004-2005 were used. In seedling bulk tests varieties/advanced lines were grouped into three categories resistant, moderately resistant and susceptible. Data from seedling bulk tests showed that DN-47 and PR 87 lines of wheat were resistant to aphid as compared to the other varieties/-advanced lines. In antixenosis tests varieties/-advanced lines were grouped into three categories, least preferred, moderately preferred, highly preferred. Lines V-01180, DN-47 and PR-84 were least preferred, sixteen varieties/-advanced lines were moderately preferred and only one variety V-9021 was found to be highly preferred.
