Effects of autotransplanted lymph node fragments on the lymphatic system in the pig model.

Secondary lymphedema often develops after removal of lymph nodes in combination with radiation therapy, in particular in patients with breast cancer, inguinal cancer, cervical cancer and melanoma. No convincing treatment for the prevention and therapy of acquired lymphedema exists so far, therefore we wanted to show the reintegration of transplanted avascular lymph node fragments in the lymphatic system and positive effects of the transplanted fragments on the restoration of the lymphatic flow in this study. A total of 26 minipigs underwent lymphadenectomy of both groins. A minimum of one lymph node was retransplanted. The lymph nodes were cut into small pieces and retransplanted in the left groin (n = 17) or in both groins (n = 9). Different retransplantation techniques were investigated, transplantation of large versus small fragments, with and without capsule. The lymph flow was evaluated 5 and 8 months after surgery, using SPECT/CT and Berlin Blue. The results were confirmed by dissection. The lymph node transplants were assessed histologically. In contrast to the lymph flow in the transplanted groin, the lymph flow in the non-transplanted groin was often malfunctioning. Large lymph node fragments were found reintegrated in the lymphatic system more often than small slices of lymph node fragments. About 5 months after surgery impairment of lymph flow was seen especially after retransplantation of small slices of lymph node fragments. In seven out of eight minipigs a dermal backflow developed in the non-transplanted groin, 8 months after surgery. Only one minipig of these groups developed dermal backflow in both groins. All lymph node fragments showed an organized structure histologically. Autologous lymph node transplantation has positive effects on the regeneration of lymph vessels and restoration of lymph flow after lymphadenectomy.

Vacuum assisted wound closure in postoperative periprosthetic groin infections: a new gold standard?

AIM: This study was designed to control the results of conservative treatment using vacuum assisted wound closure (VAWC) applied exclusively to cases of deep groin infections with involvement of alloplastic graft material. METHODS: During a 2 year period 10 patients with 11 deep inguinal infections involving alloplastic graft material were treated with supportive VAWC. Intraoperative management included extensive debridement, sartorius myoplastic and VAWC application. A retrospective case-note review was performed. Variables comorbidity, surgical management of the infection, microbiological results, complications and Doppler results were analysed. RESULTS: Six early graft infections (< 30 days after implantation) and 5 late infections were treated. In 3 cases (27.3%) the infected graft material was replaced by a silver-coated Dacron prosthesis. The mean duration of VAWC was 16+/-7.7 days; postoperative mean hospital stay was 25.3+/-8.5 days. Mean postoperative follow-up was 13.1 months with no procedure-related mortality. CONCLUSION: Even in the presence of synthetic vascular graft material, negative pressure therapy can greatly simplify challenging wound healing problems under maintenance of the alloplastic grafts. These preliminary results demonstrate the safety and effectiveness of VAWC for the treatment of deep alloplastic graft infections.