ABSTRACT
BACKGROUND: Information on lymphocyte populations (T, B, and Natural killer cells) and subpopulations (CD4 and CD8) in Morocco is scarce if not inexistent. OBJECTIVE: To establish a reference value of these cells in 242 Moroccan young adult blood donors by flow cytometry. RESULTS: Smokers had significantly higher total leukocyte count (p < 0.001), total lymphocyte count (p < 0.0001) and higher CD3+CD4+ cells (p < 0.0001). The percentage of CD3-CD56+ subsets was affected by smoking (p < 0.01). Our analysis positively correlate with previous observations of an increase of absolute CD4+ T cells, with no changes in other lymphocyte subset cells in smokers. The lymphocyte subpopulation distributions for all antigens were found to be similar to those reported in Saudi and Italian adults, while higher levels were reported for the same gender in other countries, especially Ghana and Kuwait. CONCLUSION: The international classification standards of the HIV-infected subjects according to their rates of CD4 are applicable to the present study's population.
Subject(s)
Blood Donors , Lymphocyte Subsets/immunology , Smoking/immunology , Adult , Age Distribution , Analysis of Variance , Female , Flow Cytometry , Humans , Male , Middle Aged , Morocco , Reference Values , Sex Distribution , Smoking/adverse effects , Statistics, NonparametricABSTRACT
The purpose of this report is to present the findings of a retrospective study (2008-2009) to determine the seroprevalence of hepatitis B and C virus in blood donors at the Blood Transfusion Center of Military Teaching Hospital Mohammed V in Rabat, Morocco. Samples from 19,801 consecutive blood donors were analyzed by the immuno-enzymatic method (Enzyme Linked Immunosorbent Assay, third generation). The overall seroprevalence of HBV and HCV was 0.8% and 0.2% respectively. A total of 98 units were rejected because of elevated alanine transaminase. No case of co-infection was found. From 1991 to 2010, HBV and HCV seropositivity showed a significant declining trend. In spite of the low prevalence observed, this study confirms that the risk of transfusion transmitted infection exists and thus underlines the need to implement preventive strategies to improve blood transfusion safety.
Subject(s)
Blood Donors , Hepatitis B/epidemiology , Hepatitis C/epidemiology , Adolescent , Adult , Female , Hepatitis B/blood , Hepatitis B/immunology , Hepatitis B Surface Antigens/blood , Hepatitis C/blood , Hepatitis C/immunology , Hepatitis C Antibodies/blood , Hospitals, Teaching , Humans , Male , Middle Aged , Morocco/epidemiology , Retrospective Studies , Seroepidemiologic Studies , Young AdultABSTRACT
Bacteraemia due to non Typhi Salmonella is frequent in human immunodeficiency virus (HIV infected patients). Focal vascular complications especially in patients with artheriosclerosis are rarely reported. We report the case of patient who presented a mycotic aneurysm of the abdominal aortic due to Salmonella enterica serotype enteritidis. Despite the chirurgical treatment associated with antibiotherapy, the patient evolution was fatal.
Subject(s)
Aneurysm, Infected/microbiology , Aortic Aneurysm, Abdominal/microbiology , Salmonella Infections/diagnosis , Salmonella enterica , Aneurysm, Infected/therapy , Aortic Aneurysm, Abdominal/therapy , Fatal Outcome , Humans , Male , Middle Aged , Salmonella Infections/therapyABSTRACT
The sensitivity of the detection of irregular antibodies (DIA) is one of the fundamental bases of transfusion safety. Its implementation is specified in accordance with a very specific framework that allows the use of serum or plasma. The case reported here points out a failure to detect irregular antibodies in plasma sample. Thus a low intensity anti-JK1 antibody detected and identified in serum slipped through unnoticed in plasma. The difficulties to detect and identify this antibody, well-known as a deceptive and dangerous one, are being discussed in order to better assess the limitations of DIA.
