ABSTRACT
An epithelial cell line, EC/CUHKI was established from a human squamous cell carcinoma of the oesophagus. The cells were polygonal in shape with numerous microvilli. Transmission electron microscopy (TEM) revealed the presence of desmosomes and tonofilaments. Karyotypic analysis of cells from the cell line demonstrated an aneuploid human type with a modal chromosome number of 85. Epstein-Barr viral nuclear antigen (EBNA) has not been demonstrated in the cells from the primary culture or subcultures. The tumour cells form colonies in agar and have a plating efficiency of 20%. The cells are highly invasive in vitro and are tumorigenic in nude mouse.
Subject(s)
Carcinoma, Squamous Cell/pathology , Esophageal Neoplasms/pathology , Aged , Carcinoma, Squamous Cell/genetics , Carcinoma, Squamous Cell/ultrastructure , Cell Line , Esophageal Neoplasms/genetics , Esophageal Neoplasms/ultrastructure , Humans , Male , Microscopy, ElectronABSTRACT
A case is presented of laryngeal impaction in an infant by a cervical vertebra of a toad. Symptoms were mild initially but became progressive later with secondary laryngeal edema. Early diagnosis was not made, which resulted in considerable difficulty in management. Computerized tomography (CT) scan was found to be useful in identifying the foreign body and thereby facilitating management. CT is recommended for suspected foreign bodies in the air and food passages where more conventional methods fail to make a diagnosis.
Subject(s)
Foreign Bodies/diagnostic imaging , Larynx/diagnostic imaging , Animals , Bufonidae , Humans , Infant , Male , Tomography, X-Ray ComputedABSTRACT
Effective sump drainage of high enterocutaneous fistulae, together with alimentary rest and total parenteral nutrition, is now an integral part of the modern management of patients with this condition. The low tissue reactivity of the plastic and polymer materials currently used in most drainage tubes appears however, to be counterproductive to the establishment of a discrete fistula track and control of the fistula. A case is made for the use of red rubber sump drains for enterocutaneous fistulae. The greater tissue reactivity of rubber is reviewed and confirmed by animal experimentation. A method of rubber sump drainage of enterocutaneous fistulae developed during the management of 83 such fistulae is described.
