ABSTRACT
American kestrels (Falco sparverius) were fed meat diets containing 0, 3, 6, or 12 ppm (dry weight) methylmercury chloride. Birds fed the 12-ppm diet started to show signs of neurotoxicity after 26 days and all died in 39-49 days. One male kestrel fed the 6-ppm diet died after 75 days of exposure and several others showed signs of neurotoxicity after 45 days. None of the birds fed the 3-ppm diet died or showed signs of toxicity. After 59 days of exposure, mercury concentrations in the liver, kidney, and blood of nonreproducing kestrels increased with increasing dietary concentration. Tissue concentrations of mercury also steadily increased over time in birds fed diets with 6 ppm mercury, which were necropsied at 8, 15, 29, or 59 days of exposure, reaching mean total mercury concentrations of 57, 46, and 45 ppm (wet weight) at 59 days in the liver, kidney, and whole blood, respectively. Two pairs of kestrels at each dietary concentration were allowed to breed. Eggs averaged 8.3 and 18.1 ppm (wet weight) total mercury from birds fed 3- and 6-ppm diets, respectively. Feathers grown during mercury exposure contained high concentrations of mercury: Birds fed 3- and 6-ppm diets contained 275 and 542 ppm total mercury, respectively.
Subject(s)
Falconiformes/physiology , Methylmercury Compounds/pharmacokinetics , Methylmercury Compounds/toxicity , Water Pollutants, Chemical/pharmacokinetics , Water Pollutants, Chemical/toxicity , Administration, Oral , Animal Feed , Animals , Cerebellum/drug effects , Cerebellum/pathology , Dose-Response Relationship, Drug , Feathers/chemistry , Feathers/metabolism , Female , Longevity/drug effects , Male , Methylmercury Compounds/analysis , Nervous System Diseases/chemically induced , Nervous System Diseases/pathology , Nervous System Diseases/physiopathology , Neurons/drug effects , Neurons/pathology , Ovum/chemistry , Ovum/metabolism , Reproduction/drug effects , Tissue Distribution , Toxicity Tests , Water Pollutants, Chemical/analysisABSTRACT
Ecological risk assessments for mercury (Hg) require measured and modeled information on exposure and effects. While most of this special issue focuses on the former, i.e., distribution and fate of Hg within aquatic food webs, this paper describes an approach to predict the effects of dietary methylmercury (CH3Hg) on populations of piscivorous birds. To demonstrate this approach, the U.S. Environmental Protection Agency's National Health and Environmental Effects Research Laboratory (U.S. EPA NHEERL) is working cooperatively with environmental and conservation organizations to develop models to predict CH3Hg effects on populations of the common loon, Gavia immer. Specifically, a biologically-based toxicokinetic model is being used to extrapolate CH3Hg effects on the reproduction of a tested bird species, the American kestrel (Falco sparverius), to the loon. Population models are being used to incorporate stressor effects on survival and reproduction into projections of loon population effects. Finally, habitat and spatially-explicit population models are being used to project results spatially, assess the relative importance of CH3Hg and non-chemical stressors, and produce testable predictions of the effects of biologically-available Hg on loon populations. This stepwise process provides an integrated approach to estimate the impact on wildlife populations of regulations that limit atmospherically-distributed Hg, and to develop risk-based population-level regulatory criteria.
