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1.
J Clin Pathol ; 63(2): 151-5, 2010 Feb.
Article in English | MEDLINE | ID: mdl-20154038

ABSTRACT

AIMS: Although poor thymic function leading to viral and fungal infections can be a feature of chromosome 22q11.2 deletion syndrome, most patients have relatively normal immunity. The aim of this study was to investigate which clinical and laboratory parameters best predict the likelihood of serious or recurrent infections in patients with this syndrome. METHODS: Clinical and laboratory parameters from 64 patients with 22q11.2 deletion syndrome referred to two immunology centres in the north of England were studied retrospectively. RESULTS: 31 (48%) patients had no problems with infection, 21 (33%) had bacterial infections, and 12 (19%) had recurrent or persistent thrush and/or viral enteritis and bronchiolitis, the latter suggestive of a significant T cell immunodeficiency. Patients with a history of thrush/viral infections, but not those with bacterial infections, had significantly lower CD4+ and CD8+ T lymphocyte numbers (relative risk (95% CI) 0.3 (0.1 to 0.8)) and phytohaemagglutinin mitogen responses (0.4 (0.2 to 0.8)) adjusted for age at testing. Hypoparathyroidism was associated with low T lymphocyte numbers and function (p<0.05) as well as an increased risk of thrush/viral infections (p<0.0001) after adjusting for age at testing. CONCLUSIONS: 22q11.2 syndrome patients with hypoparathyroidism are more likely to have a clinically significant T cell immunodeficiency and lower laboratory parameters of T cell function, with a higher risk of thrush and viral bronchiolitis and enteritis. Measurement of absolute CD3 count is a simple and accurate predictor of fungal/viral infection risk, with phytohaemagglutinin mitogen responses providing little or no further value in most patients.


Subject(s)
DiGeorge Syndrome/immunology , Candidiasis, Oral/complications , Candidiasis, Oral/immunology , Child , Child, Preschool , DiGeorge Syndrome/complications , Female , Humans , Hypoparathyroidism/complications , Hypoparathyroidism/immunology , Immunity, Cellular , Lymphocyte Count , Male , Opportunistic Infections/complications , Opportunistic Infections/immunology , Phenotype , Recurrence , Retrospective Studies , T-Lymphocyte Subsets/immunology , Virus Diseases/complications , Virus Diseases/immunology
2.
Rheumatology (Oxford) ; 46(10): 1612-4, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17890276

ABSTRACT

OBJECTIVES: C1q deficiency is a rare inherited defect in the early part of the complement cascade. In this report, we describe the varied clinical features of patients with this condition as well as the characteristic autoantibody profile. METHODS: A large Pakistani family with a high degree of consanguinity is described in which the father and five sons have C1q deficiency, all with different clinical manifestations. RESULTS: Clinical features of C1q deficiency can vary from almost no disease to fulminant bacterial infection and localized lupus-like skin, renal or CNS disease. Autoantibodies to ribonucleoproteins such as anti-Sm and Ro, but not dsDNA, were present. CONCLUSIONS: Awareness of the spectrum of clinical disease, autoantibody profiles and tests required to confirm the diagnosis of C1q deficiency are important if this life-threatening immunodeficiency disease is to be managed correctly.


Subject(s)
Autoantibodies/blood , Complement C1q/deficiency , Adolescent , Adult , Angioedemas, Hereditary/immunology , Child , Humans , Lupus Erythematosus, Discoid/immunology , Lupus Nephritis/immunology , Lupus Vasculitis, Central Nervous System/immunology , Magnetic Resonance Imaging , Male , Meningitis, Pneumococcal/immunology , Opportunistic Infections/immunology , Pedigree , Vasculitis/immunology , Vasculitis/pathology
3.
J Clin Pathol ; 55(10): 729-33, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12354795

ABSTRACT

This article describes a new set of revised standards for the medical laboratory, which have been produced by Clinical Pathology Accreditation (UK) Ltd (CPA). The original standards have been in use since 1992 and it was recognised that extensive revision was required. A standards revision group was established by CPA and this group used several international standards as source references, so that the resulting new standards are compatible with the most recent international reference sources. The aim is to make the assessment of medical laboratories as objective as possible in the future. CPA plans to introduce these standards in the UK in 2003 following extensive consultation with professional bodies, piloting in selected laboratories, and training of assessors.


Subject(s)
Accreditation , Laboratories, Hospital/standards , Pathology, Clinical/standards , Diffusion of Innovation , Humans , Quality Assurance, Health Care , United Kingdom
4.
Clin Exp Immunol ; 125(2): 184-9, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11529907

ABSTRACT

Toxin-positive strains of Staphylococcus aureus (T + S. aureus) are present on the skin of some but not all patients with atopic dermatitis. Many staphylococcal toxins are superantigens, which can stimulate the immune response and thus may potentially lead to the very high levels of IgE characteristic of this condition, as well as exacerbating the clinical disease. The aim of this study was to determine whether the presence of T + S. aureus on the skin of children with atopic dermatitis was associated with in vivo evidence of a heightened humoral immune response, higher IgE levels and more severe clinical disease. Toxin gene expression in S. aureus isolated from the eczematous lesions of 28 children with atopic dermatitis was assessed by PCR. Clinical and immune data were also collected from this cohort. Thirteen of the 28 children (46%) were colonized with T + S. aureus strains. The presence of T + S. aureus was associated with a significant expansion in peripheral blood CD5- B cells (P = 0.01), and the more toxin types identified the greater the B-cell expansion (P = 0.002). However, in this cohort of children with atopic dermatitis, despite th in vivo expansion of B cells in children harbouring T + S. aureus, there was no associated increase in IgE levels or in clinical disease severity scores.


Subject(s)
B-Lymphocytes/immunology , CD5 Antigens/analysis , Dermatitis, Atopic/immunology , Dermatitis, Atopic/microbiology , Enterotoxins/analysis , Staphylococcus aureus/pathogenicity , Adolescent , B-Lymphocytes/chemistry , Blood/immunology , Child , Child, Preschool , Dermatitis, Atopic/diagnosis , Eosinophils , Humans , Immunoglobulin E/blood , Immunoglobulin G/blood , Leukocyte Count , Lymphocyte Activation , Lymphocyte Count , Nasal Cavity/microbiology , Staphylococcus aureus/chemistry , Superantigens/analysis
5.
Clin Exp Immunol ; 122(1): 16-9, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11012612

ABSTRACT

The aim of this study was to investigate a previously undocumented observation, that children with atopic eczema under 9 years of age tended to have a poor antibody response to Pneumococcal vaccination. Thirty-five children (mean age 8.8 years, range 3-16 years) with moderate to severe atopic eczema but no history of systemic infection were studied retrospectively. Pneumococcal antibody responses after immunization with Pneumovax II were compared with a hospital control group consisting of 36 children (mean age 6.0 years, range 3-16 years) with recurrent upper respiratory tract infections. Only 17% of children with atopic eczema aged 3-8 years responded to Pneumovax. This response was significantly poorer than that of the controls (57%) (odds ratio 0.20, 95% confidence interval (CI) 0.05-0.84, P = 0.03). There were no significant differences in the levels of total IgG2, the component of IgG associated with protective antibody responses to Pneumococcus between the two groups. Delay in maturation of the total IgG and IgG2 antibody response to Pneumococcus is a feature in this group of children with moderately severe atopic eczema.


Subject(s)
Antibodies, Bacterial/immunology , Dermatitis, Atopic/immunology , Pneumococcal Vaccines/immunology , Adolescent , Biomarkers , Child , Child, Preschool , Humans , Immunoglobulin A/blood , Immunoglobulin A/immunology , Immunoglobulin G/blood , Immunoglobulin G/immunology , Retrospective Studies
6.
Clin Rheumatol ; 18(2): 132-5, 1999.
Article in English | MEDLINE | ID: mdl-10357118

ABSTRACT

Bronchiectasis (BR) occurs in about 3% of patients with rheumatoid arthritis (RA). Defective antibody production is a rare but well-recognised cause of both BR and inflammatory arthritis. We examined the hypothesis that subtle specific antibody defects might play a role in the pathogenesis of BR associated with RA. Identification of defects in antibody production is important because substantial benefits may be gained from immunoglobulin replacement. Specific antibody production was assessed in 20 patients with RA and BR, 20 with BR alone, 20 with RA alone and 20 healthy controls (all groups matched for age and sex). All had normal total IgG. IgA and IgM and IgG subclass levels. Specific antibody production was assessed by assay of antibodies to representative polysaccharide and protein antigens. Subjects with subprotective titres were challenged with the appropriate vaccine. Defective antibody production was defined as a subprotective level despite immunisation. Three out of 20 patients with RA and BR had a defective IgG2 response to the polysaccharide antigen, but normal responses to the protein antigen. All of the subjects in the BR alone or healthy control group had normal antibody production. Two out of 20 patients with RA alone had defective production of antibodies against both protein and polysaccharide antigens; both were receiving gold therapy, a recognised cause of functional antibody defects. It was concluded that some patients with RA and BR have functional antibody defects and may benefit from antibody replacement. An unexpectedly high proportion of patients with RA alone also have functional antibody defects, possibly secondary to gold therapy.


Subject(s)
Antibodies, Bacterial/biosynthesis , Arthritis, Rheumatoid/immunology , Bronchiectasis/immunology , Immunoglobulin Isotypes/biosynthesis , Polysaccharides, Bacterial/immunology , Adult , Aged , Antibody Formation , Arthritis, Rheumatoid/complications , Bacterial Capsules/immunology , Bronchiectasis/complications , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunization , Lipopolysaccharides/immunology , Middle Aged
7.
J R Soc Med ; 91(3): 133-4, 1998 Mar.
Article in English | MEDLINE | ID: mdl-9659324

ABSTRACT

In two children with atopic dermatitis, routine vaccination with bacillus Calmette-Guérin (BCG) was followed by severe exacerbation of skin disease. If the sequence is cause and effect, a possible mechanism is stimulation of a Th2 lymphocyte cytokine profile by the vaccine, with migration of activated lymphocytes to inflamed skin. In children with active atopic dermatitis, BCG vaccination is best deferred until remission.


Subject(s)
BCG Vaccine/adverse effects , Dermatitis, Atopic/etiology , Vaccination/adverse effects , Age Factors , Child , Dermatitis, Atopic/immunology , Female , Follow-Up Studies , Humans , Male
8.
J Qual Clin Pract ; 18(1): 75-82, 1998 Mar.
Article in English | MEDLINE | ID: mdl-9563564

ABSTRACT

The accreditation scheme established by Clinical Pathology Accreditation (UK) Ltd has been in formal operation since 1992. So far, 933 laboratories have been inspected, nearly two-thirds of all laboratories in the UK. Of these, 73% are fully accredited, 24% have outstanding conditions requiring correction, and 3% have been referred. Inspectors have identified some regional and specialty differences, most notably in histopathology and cytology. The second cycle of inspection visits is underway. Most UK departments of pathology have embraced the concept of accreditation with relative enthusiasm and there has been strong support from professional bodies, specialist societies, health service managers, the independent sector and government departments.


Subject(s)
Accreditation/organization & administration , Pathology/standards , Cell Biology/standards , Histology/standards , Humans , Quality Assurance, Health Care , Societies, Medical , State Medicine/standards , United Kingdom
9.
J Antimicrob Chemother ; 41 Suppl A: 41-6, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9511085

ABSTRACT

Complement provides a critical and multifaceted defence system against infection. Following activation, complement can clear invading microorganisms by lysis or by opsonization, which promotes recognition by complement receptors on phagocytes. Complement activation products promote a local inflammatory response and are involved in the modulation of adaptive immune responses that lead to antibody production. The host has developed regulatory mechanisms for controlling complement activation and for protecting its own cells against complement attack. Some microorganisms have also evolved ways to avoid the opsonic and lytic action of complement.


Subject(s)
Complement Activation , Complement System Proteins/immunology , Sepsis/immunology , Complement C3/immunology , Complement System Proteins/physiology , Humans , Immunoglobulin D/immunology , Immunoglobulin E/immunology , Immunoglobulin G/immunology , Immunotherapy , Phagocytosis , Receptors, Complement/physiology , Sepsis/therapy
10.
Clin Exp Immunol ; 109(1): 67-72, 1997 Jul.
Article in English | MEDLINE | ID: mdl-9218826

ABSTRACT

We have measured antibodies to pneumococcal and Haemophilus polysaccharides in a prospective study of 450 children aged 2-16 years with otitis media requiring grommets (ear tubes). Pneumococcal antibody levels were significantly higher in the 2-6 year (P < 0.004) and 7-10 year (P < 0.04) study groups in comparison with age-matched controls. There was no difference in Haemophilus antibody levels between the study and control group children for the age groups 2-6 years and 11-16 years. Haemophilus antibody levels were significantly lower in the 7-10 year (P < 0.003) group in comparison with age-matched controls. Eighty-eight out of 450 (19.6%) children had pneumococcal antibody levels below the 25th percentile. Nineteen out of 88 (21.6%) children with pneumococcal antibody levels below the 25th centile were test immunized with 23 valent Pneumococcal polysaccharide and unconjugated Haemophilus type b capsular polysaccharide. Of these 19 children (aged 4-11 years), five mounted suboptimal responses to both polysaccharide antigens, whilst one child failed to respond to Haemophilus polysaccharide alone. There was no significant difference in the prevalence of IgG subclass deficiency between the normal responders and poor responders to immunization (P = 0.12). We found no evidence of specific polysaccharide antibody deficiency in the vast majority of the 450 children studied. However, the significance of poor antibody responses to test immunization in a small minority of children with otitis media is unclear. Long-term follow up of these children is required to determine whether poor immunization responses herald the development of frank antibody deficiency.


Subject(s)
Antibodies, Bacterial/analysis , Haemophilus influenzae/immunology , Otitis Media/immunology , Polysaccharides, Bacterial/immunology , Streptococcus pneumoniae/immunology , Antigens, Bacterial/administration & dosage , Antigens, Bacterial/immunology , Child , Child, Preschool , Humans , Immunization , Immunoglobulin G/analysis , Immunoglobulin G/immunology , Middle Ear Ventilation , Otitis Media/therapy , Polysaccharides, Bacterial/administration & dosage , Prospective Studies
11.
Ann Rheum Dis ; 55(9): 616-21, 1996 Sep.
Article in English | MEDLINE | ID: mdl-8882131

ABSTRACT

OBJECTIVE: To perform a clinical and immunological study of patients with rheumatoid arthritis who develop subnormal serum immunoglobulins on gold treatment; to clarify the nature of the defect in antibody production and determine the natural history of this adverse reaction; to use this information to suggest guidelines for the detection, investigation, and management of this complication. METHODS: 22 patients who developed subnormal levels of one or more immunoglobulin isotypes while receiving gold treatment were recruited over a 10 year period from the practice of a single rheumatologist. Antibody production was assessed by measurement of total immunoglobulins and of specific antibody production against polysaccharide and protein antigens, with test immunisation if necessary. RESULTS: Two broad patterns of antibody deficiency were identified: (1) (n = 11) mild, affecting only one immunoglobulin isotype and with normal specific antibody production. These patients were in general able to continue gold without further deterioration in antibody production. (2) (n = 11) severe, affecting two or three immunoglobulin isotypes, with defective specific antibody production. Six patients developed significant infections and were treated with immunoglobulin. Gold was discontinued in all. Normal antibody production recovered in nine patients, and in all but one followed for more than one year. No relation was seen between duration/dose of gold and antibody deficiency. CONCLUSIONS: Gold-induced antibody deficiency may be more common than usually recognised. A spectrum of deficiency exists, with some patients developing infective complications. Antibody production should be monitored in patients on gold treatment.


Subject(s)
Arthritis, Rheumatoid/immunology , Gold Sodium Thiomalate/adverse effects , Immunoglobulins/blood , Adult , Aged , Aged, 80 and over , Antibody Formation/drug effects , Arthritis, Rheumatoid/drug therapy , Female , Gold Sodium Thiomalate/therapeutic use , Humans , Immunoglobulin A/blood , Immunoglobulin G/blood , Immunoglobulin M/blood , Male , Middle Aged
12.
J Infect ; 31(1): 67-8, 1995 Jul.
Article in English | MEDLINE | ID: mdl-8522838

ABSTRACT

A 32-year-old male presented with two episodes of meningococcal septicaemia, each of which was caused by a different serogroup of Neisseria meningitidis. Examination of the alternative pathway of complement revealed the rare X-linked disorder properdin deficiency (PD). Meningococcal Infection in complement deficiency states is discussed and the unusual features of this case are highlighted.


Subject(s)
Bacteremia/microbiology , Meningococcal Infections/microbiology , Neisseria meningitidis/isolation & purification , Properdin/deficiency , Adult , Bacteremia/drug therapy , Humans , Injections, Intravenous , Male , Meningococcal Infections/drug therapy , Penicillin G/administration & dosage , Penicillin G/therapeutic use , Penicillins/administration & dosage , Penicillins/therapeutic use
13.
Clin Exp Immunol ; 97(2): 239-41, 1994 Aug.
Article in English | MEDLINE | ID: mdl-8050171

ABSTRACT

Serum levels of the soluble form of the low-affinity receptor for IgE (FcERII, CD23) (sCD23) are elevated in autoimmune conditions associated with hypergammaglobulinaemia and B cell hyperactivity. Very high levels of sCD23 are found in patients with B-chronic lymphatic leukaemia (B-CLL) who are, however, frequently hypogammaglobulinaemic. We therefore compared the serum levels of sCD23 in healthy controls (n = 33) with three conditions associated with hypogammaglobulinaemia (HGG) and varying B cell numbers: X-linked agammaglobulinaemia (XLA, n = 12), common variable immunodeficiency (CVI, n = 20) and B-chronic lymphatic leukaemia (n = 33). Serum levels of sCD23 showed a significant correlation with the CD19+ B cell count in both normals and patients with CVI (r = 0.65, P < 0.0001). Amongst the different clinical groups, serum levels of sCD23 were increased in the order XLA < CVI < normals < CLL (medians 2.5, 7.7, 11.1 and 540, respectively; P < 0.001 for all comparisons except CVI versus normals P < 0.03 in a one-tailed test). In the CVI group, serum sCD23 was lowest amongst four patients with low B cell numbers. There was no overlap in sCD23 between patients with XLA and this subgroup of CVI patients. Serum sCD23 is, therefore, derived predominantly from B cells, and is significantly related to the peripheral blood B cell count.


Subject(s)
Agammaglobulinemia/immunology , B-Lymphocytes/immunology , Receptors, IgE/metabolism , Agammaglobulinemia/genetics , Common Variable Immunodeficiency/immunology , Female , Genetic Linkage , Humans , Leukemia, Lymphocytic, Chronic, B-Cell/immunology , Leukocyte Count , Male , X Chromosome
14.
Ann Clin Biochem ; 31 ( Pt 3): 249-54, 1994 May.
Article in English | MEDLINE | ID: mdl-8067665

ABSTRACT

The measurement of inorganic phosphate using an unmodified acid/molybdate assay is known to be subject to interference when paraproteinaemia exists. This phenomenon, due to precipitation in the reaction mixture, is not common to all paraproteins. We studied sera from 35 patients to determine whether interference in the assay was related to particular electrophysical characteristics of the paraproteins. There were spuriously elevated phosphate concentrations in 48.6% of the sera assayed. This could not be related to a direct effect of light chain type, electrical charge or IgG subclass. No IgA paraproteins were found to cause interference but there were immunoglobulin G (IgG) and immunoglobulin M (IgM) paraproteins in both the 'interfering' and 'non-interfering' groups. The median paraprotein concentration was similar in both groups but, where interference occurred, the degree increased in line with the paraprotein concentration. Although it does not seem possible to predict which samples will cause interference, it is important that the clinical implications of this problem are appreciated.


Subject(s)
Antibodies, Monoclonal/chemistry , Immunoglobulin G/chemistry , Phosphates/blood , Electrochemistry , Humans , Paraproteinemias/blood
15.
J Clin Pathol ; 46(12): 1113-5, 1993 Dec.
Article in English | MEDLINE | ID: mdl-8282835

ABSTRACT

AIMS: To determine whether the presence of anticardiolipin antibodies in patients with suspected myocardial infarction is predictive of complications during hospital stay or after discharge. METHODS: Anticardiolipin antibodies were serially measured in a cohort of 111 patients, from the time of admission to the coronary care until till eight weeks after discharge. Associations with fatal and non-fatal cardiac complications were documented. RESULTS: The incidence of raised titres of IgG and IgM anticardiolipin antibodies (ACA) in patients with myocardial infarction was comparable with that in patients with ischaemic heart disease. ACA titres in patients with a previous myocardial infarct were not significantly different from those found in patients without a previous history of infarction. Over the period of the study, ACA titres in the myocardial infarct group did not change significantly from those recorded on admission, nor did those patients with raised ACA titres have a higher prevalence of complications in hospital or in the early period after discharge. CONCLUSIONS: There is no evidence that patients with an acute or previous myocardial infarct have higher ACA titres than those found in patients with ischaemic heart disease. Raised ACA titres soon after myocardial infarction do not influence immediate patient outcome.


Subject(s)
Antibodies, Anticardiolipin/blood , Immunoglobulin G/blood , Immunoglobulin M/blood , Myocardial Infarction/immunology , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Myocardial Infarction/blood , Myocardial Ischemia/immunology , Prognosis
16.
Mutat Res ; 290(2): 255-64, 1993 Dec.
Article in English | MEDLINE | ID: mdl-7694117

ABSTRACT

From more than 500 tumours reported in human primary immune deficiencies a majority has been observed in two disorders: ataxia telangiectasia (A-T) and common variable immune deficiency (CVID). Since both diseases have an increased risk of lymphomas/leukaemias and gastrointestinal tumours, suggesting a common risk factor, and the cells derived from A-T patients exhibit an increased chromosomal radiosensitivity we analysed chromosome damage in the G2 lymphocytes of 24 CVID patients and 21 controls after X-irradiation in vitro. There was a significant difference in mean aberration yields between patients and controls. Three CVID patients had yields higher than the mean + 3SD of the controls. Six patients but only one control had yields higher than the mean + 2SD of controls. The patient with the highest chromosomal radiosensitivity subsequently developed a lymphoma. Repeat assays on the same blood sample, with a 24-h delay in setting up the second culture, showed as much variability for control donors as the variation between control donors although for CVID patients inter-individual variation was greater than the difference between results of repeat samples. There was a weak positive correlation between radiosensitivity and age of donor. Chromosomal radiosensitivity of five patients with X-linked hypogammaglobulinaemia was not different from healthy donors. The mean mitotic index (MI) for unirradiated samples from CVID patients was significantly lower than for controls and there was an inverse relationship between MI and aberration yields in the patients, but not in controls. We suggest that the defect in CVID patients that reduces response to mitogenic stimuli may have mechanism(s) in common with those involved in cellular repair processes.


Subject(s)
Chromosome Aberrations , Chromosomes, Human/radiation effects , Common Variable Immunodeficiency/genetics , Radiation Tolerance , Adult , Age Factors , Aged , Analysis of Variance , Case-Control Studies , Cells, Cultured , Chromatids/radiation effects , Female , Humans , Lymphocytes/radiation effects , Male , Middle Aged , Mitotic Index , Neoplasms, Radiation-Induced/genetics , Reproducibility of Results
17.
Clin Exp Immunol ; 94(1): 17-20, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8403502

ABSTRACT

Using a flow cytometric method, CD4+, CD8+, alpha beta TCR+ and TCR variable region gene product (TVRGP)-specific T cells were analysed in healthy heterosexual males (HHeM), HIV-seronegative homosexual males (SNHM), asymptomatic seropositive homosexual males (ASPH) and homosexual males with AIDS who were either well (AIDS-A), or unwell in hospital (AIDS-B). Total CD4+ and CD8+ T cell numbers were similar in HHeM and SNHM. CD4+ T cells were significantly reduced in ASPH relative to both HHeM and SNHM and in AIDS-A and AIDS-B relative to SNHM. TVRGP-specific T cells expressed as a percentage of TCR alpha beta + cells showed no significant difference in HHeM, SNHM and AIDS-B. The proportion of alpha beta + cells expressing the V beta 5.1, V beta 12 and V alpha 2 gene product (GP) was, however, significantly reduced in ASPH and AIDS-B relative to HHeM, SNHM and AIDS-A. Possible causes of TVRGP-specific T cell deletion are discussed.


Subject(s)
HIV Infections/immunology , Receptors, Antigen, T-Cell, alpha-beta/analysis , T-Lymphocytes/immunology , CD4 Antigens/analysis , CD8 Antigens/analysis , Homosexuality , Humans , Male
18.
J Clin Pathol ; 46(9): 846-8, 1993 Sep.
Article in English | MEDLINE | ID: mdl-7901239

ABSTRACT

AIMS: To determine whether the routine measurement of lymphocyte transformation responses to mitogenic stimuli provide any information additional to that available from routine T cell CD4 and CD8 analysis in patients with HIV infection. METHODS: The case records of 197 immunologically investigated HIV seropositive patients were reviewed. The influence of disease stage on T lymphocyte subsets and lymphocyte transformation responses (LyTR) to phytohaemagglutinin (PHA) and Pokeweed mitogen was assessed. RESULTS: The median CD3 and CD4 counts and LyTR to PHA and Pokeweed mitogen were highest in patients with persistent generalised lymphadenopathy (PGL) and decreased progressively in the order: asymptomatic patients, those with ARC, those with AIDS. LyTR to PHA was preserved in over 70% of all patients, but the response to Pokeweed mitogen was depressed in 8% of patients with PGL, 34% of asymptomatic patients, 68% of those with ARC and 78% of those with AIDS. Subnormal values of both CD4 + T cells and LyTR to Pokeweed mitogen were more common in patients with ARC and AIDS (68%) than in those who were asymptomatic or had PGL (20%). CONCLUSIONS: CD4 T cell analysis and LyTR to Pokeweed mitogen, but not to PHA, both correlate with disease states in patients with HIV infection.


Subject(s)
HIV Infections/immunology , Lymphocyte Activation/immunology , Phytohemagglutinins/immunology , Pokeweed Mitogens/immunology , AIDS-Related Complex/immunology , Acquired Immunodeficiency Syndrome/immunology , CD4-CD8 Ratio , CD4-Positive T-Lymphocytes/immunology , Female , Humans , Leukocyte Count , Male , T-Lymphocyte Subsets/immunology
19.
J Clin Pathol ; 46(7): 678-80, 1993 Jul.
Article in English | MEDLINE | ID: mdl-8157762

ABSTRACT

A 51 year old man with Waldenström's macroglobulinaemia presented with a malabsorptive syndrome related to extensive small bowel lymphangiectasia caused by immunoglobulin accumulation. The patient's plasma had strong lupus anticoagulant activity and the IgM lambda paraprotein displayed specificity for the negatively charged phospholipids phosphatidyl serine and phosphatidyl inositol, as well as the neutral phosphatidic acid. Despite treatment for the macroglobulinaemia the patient died and at necropsy was found to have myocardial ischaemia and segmental infarcts in the spleen and kidney. The coexistence of these relatively rare findings suggests a possible association between Waldenström's macroglobulinaemia with gastrointestinal manifestations and paraprotein specificity for phospholipid.


Subject(s)
Immunoglobulin M/immunology , Immunoglobulin lambda-Chains/immunology , Lupus Coagulation Inhibitor/immunology , Lymphangiectasis, Intestinal/etiology , Malabsorption Syndromes/etiology , Paraproteins/immunology , Waldenstrom Macroglobulinemia/immunology , Antibody Specificity , Humans , Male , Middle Aged , Phospholipids/immunology , Waldenstrom Macroglobulinemia/complications
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