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1.
PLoS Genet ; 4(3): e1000026, 2008 Mar 07.
Article in English | MEDLINE | ID: mdl-18369445

ABSTRACT

A fundamental problem in developmental biology concerns how multipotent precursors choose specific fates. Neural crest cells (NCCs) are multipotent, yet the mechanisms driving specific fate choices remain incompletely understood. Sox10 is required for specification of neural cells and melanocytes from NCCs. Like sox10 mutants, zebrafish shady mutants lack iridophores; we have proposed that sox10 and shady are required for iridophore specification from NCCs. We show using diverse approaches that shady encodes zebrafish leukocyte tyrosine kinase (Ltk). Cell transplantation studies show that Ltk acts cell-autonomously within the iridophore lineage. Consistent with this, ltk is expressed in a subset of NCCs, before becoming restricted to the iridophore lineage. Marker analysis reveals a primary defect in iridophore specification in ltk mutants. We saw no evidence for a fate-shift of neural crest cells into other pigment cell fates and some NCCs were subsequently lost by apoptosis. These features are also characteristic of the neural crest cell phenotype in sox10 mutants, leading us to examine iridophores in sox10 mutants. As expected, sox10 mutants largely lacked iridophore markers at late stages. In addition, sox10 mutants unexpectedly showed more ltk-expressing cells than wild-type siblings. These cells remained in a premigratory position and expressed sox10 but not the earliest neural crest markers and may represent multipotent, but partially-restricted, progenitors. In summary, we have discovered a novel signalling pathway in NCC development and demonstrate fate specification of iridophores as the first identified role for Ltk.


Subject(s)
Protein-Tyrosine Kinases/metabolism , Zebrafish/embryology , Zebrafish/metabolism , Alleles , Animals , Apoptosis/genetics , Carrier Proteins/genetics , Carrier Proteins/metabolism , Chromosome Mapping , Embryonic Stem Cells/cytology , Embryonic Stem Cells/enzymology , Gene Expression Regulation, Developmental , High Mobility Group Proteins/genetics , High Mobility Group Proteins/metabolism , Leukocytes/enzymology , Melanocytes/cytology , Melanocytes/enzymology , Models, Biological , Multipotent Stem Cells/cytology , Multipotent Stem Cells/enzymology , Mutation , Neural Crest/cytology , Neural Crest/embryology , Neural Crest/enzymology , Phylogeny , Protein-Tyrosine Kinases/genetics , SOXE Transcription Factors , Zebrafish/genetics , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
2.
Genes Dev ; 18(13): 1565-76, 2004 Jul 01.
Article in English | MEDLINE | ID: mdl-15198976

ABSTRACT

Signaling by lipid-modified secreted glycoproteins of the Hedgehog family play fundamental roles during pattern formation in animal development and in humans; dysfunction of Hedgehog pathway components is frequently associated with a variety of congenital abnormalities and cancer. Transcriptional regulation of Hedgehog target genes is mediated by members of the Gli zinc-finger transcription factors. The relative nuclear concentrations of Gli activator (Gli(act)) and repressor (Gli(rep)) forms, together with their nucleocytoplasmic trafficking, appear to be critical determinants for target gene expression. Whereas such stringent controls of Gli activity are critical in ensuring appropriate levels of pathway activation, the mechanisms by which these processes are regulated remain inadequately understood. Here, using genetic analysis, we show that the zebrafish iguana gene product acts downstream of the Smoothened protein to modulate Gli activity in the somites of the developing embryo. Positional cloning reveals that iguana encodes the zebrafish ortholog of Dzip1, a novel zinc-finger/coiled-coil domain protein that we show can shuttle between the cytoplasm and nucleus in a manner correlated with Hedgehog pathway activity.


Subject(s)
Carrier Proteins/metabolism , Drosophila Proteins , Signal Transduction , Trans-Activators/metabolism , Zebrafish Proteins/metabolism , Zebrafish/embryology , Zinc Fingers , Animals , Carrier Proteins/genetics , Cyclic AMP-Dependent Protein Kinases/metabolism , Embryo, Nonmammalian , Female , Gene Expression Regulation, Developmental , Hedgehog Proteins , Molecular Sequence Data , Mutation , Oncogene Proteins/genetics , Oncogene Proteins/metabolism , Protein Structure, Tertiary , Receptors, G-Protein-Coupled/metabolism , Repressor Proteins/genetics , Repressor Proteins/metabolism , Smoothened Receptor , Trans-Activators/genetics , Transcription Factors/genetics , Transcription Factors/metabolism , Zebrafish Proteins/genetics , Zinc Finger Protein GLI1 , Zinc Finger Protein Gli2
3.
Development ; 130(20): 5043-52, 2003 Oct.
Article in English | MEDLINE | ID: mdl-12952905

ABSTRACT

The van gogh (vgo) mutant in zebrafish is characterized by defects in the ear, pharyngeal arches and associated structures such as the thymus. We show that vgo is caused by a mutation in tbx1, a member of the large family of T-box genes. tbx1 has been recently suggested to be a major contributor to the cardiovascular defects in DiGeorge deletion syndrome (DGS) in humans, a syndrome in which several neural crest derivatives are affected in the pharyngeal arches. Using cell transplantation studies, we demonstrate that vgo/tbx1 acts cell autonomously in the pharyngeal mesendoderm and influences the development of neural crest-derived cartilages secondarily. Furthermore, we provide evidence for regulatory interactions between vgo/tbx1 and edn1 and hand2, genes that are implicated in the control of pharyngeal arch development and in the etiology of DGS.


Subject(s)
DiGeorge Syndrome/genetics , T-Box Domain Proteins/genetics , Zebrafish/metabolism , Amino Acid Sequence , Animals , Branchial Region/metabolism , Ear/embryology , Endoderm/metabolism , Humans , Mesoderm/metabolism , Molecular Sequence Data , Mutation , Sequence Deletion , T-Box Domain Proteins/metabolism
4.
Development ; 129(21): 5065-79, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12397114

ABSTRACT

The molecular genetic mechanisms of cartilage construction are incompletely understood. Zebrafish embryos homozygous for jellyfish (jef) mutations show craniofacial defects and lack cartilage elements of the neurocranium, pharyngeal arches, and pectoral girdle similar to humans with campomelic dysplasia. We show that two alleles of jef contain mutations in sox9a, one of two zebrafish orthologs of the human transcription factor SOX9. A mutation induced by ethyl nitrosourea changed a conserved nucleotide at a splice junction and severely reduced splicing of sox9a transcript. A retrovirus insertion into sox9a disrupted its DNA-binding domain. Inhibiting splicing of the sox9a transcript in wild-type embryos with splice site-directed morpholino antisense oligonucleotides produced a phenotype like jef mutant larvae, and caused sox9a transcript to accumulate in the nucleus; this accumulation can serve as an assay for the efficacy of a morpholino independent of phenotype. RNase-protection assays showed that in morpholino-injected animals, the percent of splicing inhibition decreased from 80% at 28 hours post fertilization to 45% by 4 days. Homozygous mutant embryos had greatly reduced quantities of col2a1 message, the major collagen of cartilage. Analysis of dlx2 expression showed that neural crest specification and migration was normal in jef (sox9a) embryos. Confocal images of living embryos stained with BODIPY-ceramide revealed at single-cell resolution the formation of precartilage condensations in mutant embryos. Besides the lack of overt cartilage differentiation, pharyngeal arch condensations in jef (sox9a) mutants lacked three specific morphogenetic behaviors: the stacking of chondrocytes into orderly arrays, the individuation of pharyngeal cartilage organs and the proper shaping of individual cartilages. Despite the severe reduction of cartilages, analysis of titin expression showed normal muscle patterning in jef (sox9a) mutants. Likewise, calcein labeling revealed that early bone formation was largely unaffected in jef (sox9a) mutants. These studies show that jef (sox9a) is essential for both morphogenesis of condensations and overt cartilage differentiation.


Subject(s)
Cartilage/embryology , High Mobility Group Proteins/genetics , Transcription Factors/genetics , Zebrafish/embryology , Zebrafish/genetics , Alleles , Animals , Base Sequence , Bone Development/genetics , Cartilage/abnormalities , Cartilage/growth & development , Chondrogenesis/genetics , Chondrogenesis/physiology , DNA, Complementary/genetics , Disease Models, Animal , Gene Duplication , Gene Expression Regulation, Developmental , High Mobility Group Proteins/physiology , Humans , Muscles/embryology , Mutation , Oligodeoxyribonucleotides, Antisense/genetics , Oligodeoxyribonucleotides, Antisense/pharmacology , Osteochondrodysplasias/embryology , Osteochondrodysplasias/genetics , Pharynx/embryology , RNA Splicing/drug effects , SOX9 Transcription Factor , Transcription Factors/physiology , Zebrafish/growth & development
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