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J Feline Med Surg ; 11(12): 1031-4, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19818660

ABSTRACT

The prevalence of feline coronavirus (FCoV) was studied in two catteries in Malaysia. Rectal swabs or faecal samples were collected from a total of 44 clinically healthy Persian purebred and mix-breed cats. RNA extracted from the faecal material was subjected to a reverse transcription-polymerase chain reaction (RT-PCR) using primers flanking for a conserved region of the virus genome. The overall prevalence of FCoV infection was 84% and the infection rate was higher in Persian purebred cats (96%) than mix-breed cats (70%). There was no significant association between the age or gender of tested cats and shedding the virus. This study is the first PCR-based survey for FCoV in Malaysia and showed the ubiquitous presence of FCoV in Malaysian cat colonies.


Subject(s)
Cat Diseases/diagnosis , Cat Diseases/epidemiology , Coronavirus Infections/veterinary , Coronavirus, Feline/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Animals , Cat Diseases/virology , Cats , Coronavirus Infections/diagnosis , Coronavirus Infections/epidemiology , Coronavirus Infections/virology , Feces/virology , Female , Malaysia/epidemiology , Male , Prevalence , RNA, Viral/analysis , Sensitivity and Specificity
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