ABSTRACT
BACKGROUND: Meniscus tissue engineering has provided a great potential treatment for meniscal injuries. However, few scaffolds in meniscus tissue engineering have matched the mechanical properties of native meniscus. OBJECTIVE: In this study, we developed a composite scaffold using decellularized meniscus extracellular matrix (DMECM) and gelatin/chitosan (G/C) to explore a preferable ratio to enhance the elastic modulus and cytotoxicity properties of scaffolds. METHODS: The microstructure, porosity, cytotoxicity, and strength of the composite scaffolds were evaluated. The micro-architectures of the samples were evaluated using scanning electron microscope (SEM). Fourier Transform Infrared analysis (FTIR) was used to confirm the chemical structure with different type composite scaffolds. The compressive elastic modulus of all the scaffolds were measured by the universal tensile testing machine DNS300. Calcein-AM (fluorescent green) and propidium iodide (fluorescent red) were used to stain live cells and dead cells. Morphology and spatial distribution of cells within scaffolds were observed by confocal laser scanning microscopy FV 1000. RESULTS: SEM showed that the composite scaffolds had suitable porous structure. CCK-8 and live/dead staining demonstrated that the composite scaffolds had no cytotoxicity and could promote bone marrow mesenchymal stem cells (BMSCs) proliferation. The FTIR results demonstrated the successful mixing of these two elements, and the addition of DMECM improved the elastic modulus and cytotoxicity of G/C composite scaffolds. CONCLUSIONS: This study developed a composite scaffold using DMECM and G/C, and demonstrated that it might be suitable for meniscal tissue engineering application.
Subject(s)
Chitosan/chemistry , Extracellular Matrix/chemistry , Gelatin/chemistry , Meniscus/cytology , Mesenchymal Stem Cells/cytology , Tissue Engineering/methods , Tissue Scaffolds/chemistry , Animals , Cell Survival , Cells, Cultured , Elastic Modulus , Materials Testing , Meniscus/chemistry , Porosity , SwineABSTRACT
@#ObjectiveTo apply the Schwann cell neurilemma channel(SCNC) in promoting the regeneration of injuried spinal cord.MethodsSpinal cords of rats after laminectomy at T8/9 were divided in 3 groups randomly: 10 rats were transplanted with SCNC(Group A);10 rats only made models(Group B);10 rats were as normal control group(Group C).All rats received behaviour survey and inclined plane test every week 8 weeks after operation.6 weeks after operation,they were measured with cortical somatosensory evoked potential(CSEP).And 8 weeks after operation,all specimens were studied histologically.ResultsCSEP could be recorded in group A and C,but not in group B.There were marked differenties between group A or C and group B in behavior survey,inclined plane test.Amount of nerve fibers regenerated through the injury gap in group A was significantly larger than that in group B.ConclusionSCNC could facilitate axonal regeneration and promote the repairing of injuried spinal cord.
ABSTRACT
@#ObjectiveTo explore the effect and feasibility of autograft and allograft bone and cartilage transplantation.Methods63 rabbits were made experimental defect in the same side knee joint,and were divided randomly into group A(autograft group),group B(allograft group) and group C(control group).Group A was divided further into 2 column limiting motion subgroup(A2M group),not limiting motion subgroup(A2N group) and 4 column subgroup(A4M group).In the same way,group B was divided further into B2M group,B2N group and B4M group.Every subgroup included nine rabbits,and the transplantation was made in no weight-bear area of knee joint.The generally observation were made,the joint movable range and the thickness of the repairing tissue were measured,histology change of transplanted tissue(through light,electronic microscope)were observed.ResultsGroup A had a satisfactory result,and the result of no limiting groups were better.The result of A4M group was better than A2M group (P≤0.05).The result of group B was not good.ConclusionThe bone-cartilage column of small diameter and general number are beneficial in repairing the defect of joint cartilage.The allograft bone and cartilage transplantation can cause seriously immunoreaction and the absorption of cartilage pole,and is detrimental to repair the defect of joint cartilage.
