ABSTRACT
A system for testing the biocompatibility of synthetic oxygen carriers is described including tests of hemolysis, complement activation and proliferation of cell lines or lymphocytes. Of 17 surfactants tested in this system, 6 were compatible in all tests while the other compounds showed individually differing patterns of incompatibility. We conclude that, in order to conduct a meaningful screening, a series of different assays has to be applied. In addition to our system, further assays, covering cytokine induction and phagocytosis should be attempted.
Subject(s)
Blood Substitutes/toxicity , Complement Activation/drug effects , Fluorocarbons/toxicity , Hemolysis/drug effects , Lymphocyte Activation/drug effects , Materials Testing , Surface-Active Agents/toxicity , Cell Line , Humans , Poloxalene/toxicity , Structure-Activity RelationshipABSTRACT
A system for testing the biocompatibility of synthetic oxygen carriers is described including tests of hemolysis, complement activation and proliferation of cell lines or lymphocytes. Of 17 surfactants tested in this system, 6 were compatible in all tests while the other compounds showed individually differing patterns of incompatibility. We conclude that, in order to conduct a meaningful screening, a series of different assays has to be applied. Addition to our system of further assays covering cytokine induction and phagocytosis should be attempted.
Subject(s)
Biocompatible Materials , Blood Substitutes , Fluorocarbons , Oxygen/blood , Hemolysis/immunology , Humans , Lymphocyte Activation/immunology , Structure-Activity RelationshipABSTRACT
New surfactants have been synthesized with perfluorohexyl-groups as hydrophobic tail, a polyethyleneglycol-methylether as hydrophilic head and a prolongator between them. The prolongator units are -C4H8-, -C2H4OC5H10-, -C2H4OC3H6C(O)- and -CH2CH(OEt)CH2-. The surface tension, CMC and the interfacial tension water/perfluorocarbon were measured and the surface excess concentrations of the surfactant molecules were calculated. The influence of the surfactants on in vitro proliferation of human cell lines Hela and Molt 4 was investigated as a parameter for biocompatibility. The influence of the surfactants led to a reduction of cell proliferation depending on the concentration and chemical nature of the surfactant. Thus the prolongators changed the properties of the surfactants. They reduced the interfacial tension water/perfluorocarbon and improved the biocompatibility of surfactants.
