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1.
Georgian Med News ; (348): 54-56, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38807391

ABSTRACT

The use of tourniquet is common in orthopaedic surgeries as it reduces blood loss, enhances visualization of the operating field, and leads to quicker procedures. However, the use of tourniquet has certain risks which can be avoided by following guidelines like British Orthopaedic Association Standards for Trauma (BOAST) guidelines for safe use of tourniquet. This audit study was done in a District general hospital to check the compliance of two trauma theatres with BOAST guidelines. The audit found that there was poor documentation of tourniquet details in the operation notes (10%). Regarding tourniquet time and pressure, the compliance in the two theatres was 95 % & 97.5 %. The recommendations of this audit were to use a template to improve documentation of tourniquet details in the operation notes and training of theatre staff on BOAST guidelines for safe use of tourniquet.


Subject(s)
Hospitals, District , Medical Audit , Orthopedic Procedures , Tourniquets , Humans , Orthopedic Procedures/adverse effects , United Kingdom , Operating Rooms/standards , Guideline Adherence/statistics & numerical data , Blood Loss, Surgical/prevention & control
2.
Georgian Med News ; (348): 91-93, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38807399

ABSTRACT

The restoration of the joint line is important for a good functional outcome after a Total Knee Arthroplasty(TKA). Knee joint biomechanics need to be restored as near normal as possible. Joint line elevation leads to anterior knee pain, decrease in range of motion, patella baja ,mid-flexion instability and impingement of patellar tendon. Joint line depression on the other hand leads to patella alta, risk of patellar subluxation and mid-flexion instability of the knee. Various studies have demonstrated various range of acceptable joint line variation but there is no clear acceptable range of joint line variation. More studies are required for establishing the acceptable range of joint line variation and standard practices should be established for arthroplasty surgeons for preventing variation of joint line.


Subject(s)
Arthroplasty, Replacement, Knee , Knee Joint , Range of Motion, Articular , Humans , Range of Motion, Articular/physiology , Knee Joint/surgery , Knee Joint/physiopathology , Biomechanical Phenomena , Patella/surgery , Joint Instability/surgery , Joint Instability/physiopathology
3.
Georgian Med News ; (347): 122-124, 2024 Feb.
Article in English | MEDLINE | ID: mdl-38609127

ABSTRACT

Fractures of the metacarpal particularly the 5th metacarpal is quite common among all hand fractures and has a high incidence in male adult population. Proper management of these fractures plays a key role in rehabilitation and early return to work thus reducing the economic burden. Treatment of these injuries depends on the type of injury: whether it is a closed/open fracture, degree of angulation at the fracture site and also mal-rotation and shortening of the finger. Non-operative management is suitable for fractures which are closed, non-displaced and without angulation or rotation. Open fractures, fractures with angulation and/or mal-rotation and fractures with neuro-vascular injury are more suitable for operative management. The acceptable angulation for conservative management for most studies is 70 degrees. Buddy strapping with a Futura splint provides good functional results. In fractures requiring operative intervention, K-wire fixation is a minimally invasive method of fixation, which in most cases has good functional results. Plate and screw fixation, however, is preferred for cases with significant comminution or multiple metacarpal fractures.


Subject(s)
Fractures, Closed , Fractures, Open , Adult , Humans , Male , Bone Screws , Conservative Treatment
4.
Georgian Med News ; (347): 149-150, 2024 Feb.
Article in English | MEDLINE | ID: mdl-38609132

ABSTRACT

Traditionally, it was believed that both proximal and distal locking are essential for achieving stability during intra-medullary fixation for extra-capsular hip fractures. However, recent literature has presented varying perspectives on the necessity of distal locking. Distal locking plays a significant role in managing hip fractures with uncertainties regarding longitudinal and rotational stability. This includes cases of comminuted intertrochanteric fractures with subtrochanteric extension, reverse oblique and high oblique fractures, broad medullary canals, comminution of the lateral wall, diaphyseal fractures, and large posteromedial fragments extending below the level of the lesser trochanter. In stable pertrochanteric fractures, with the lag screw passing through the lateral cortex of the distal fragment, may not require a distal locking screw. Distal locking has been associated with potential complications, including irritation of the fascia lata, prolonged operation time, increased radiation exposure, greater blood loss, implant loosening, secondary femoral stress fractures, and damage to the femoral artery. Thus, although distal locking is of doubtful significance in stable pertrochanteric fractures it is essential in unstable fracture patterns.


Subject(s)
Femoral Fractures , Fracture Fixation, Intramedullary , Hip Fractures , Humans , Hip Fractures/diagnostic imaging , Hip Fractures/surgery , Bone Screws , Femoral Artery
5.
Bratisl Lek Listy ; 121(10): 717-721, 2020.
Article in English | MEDLINE | ID: mdl-32955903

ABSTRACT

OBJECTIVES: Abdominal aortic aneurysm (AAA) and its complications are among the most serious cardiovascular diseases and its occurrence has risen sharply in recent years. The aim of this pilot study is to explore the relationship between the methylation of matrix metalloproteinases and tissue inhibitors of the metalloproteinases genes' promoter region, and abdominal aortic aneurysm (AAA) through the detection of the methylation status of MMP2, TIMP2, TIMP1, and MMP9 genes in peripheral blood. METHODS: The study included 43 males with verified AAA (case group) and 34 healthy males (control group). The methylation status of the genes' promoter region was detected by methylation-specific polymerase chain reaction (MS-PCR). RESULTS: In adominal aortic aneurysm patients, the methylation ratio of MMP2 gene was positive in 9.3 % (4 cases), 2.3 % (1 case) had methylated TIMP2 gene, 7.0 % (3 cases) had methylated TIMP1 gene, while the methylation ratio of MMP9 gene was positive in 93.0 % (40 cases). In the control group, MMP2 gene was found to be methylated in 5.9 % (2 cases), 5.9 % of cases had methylated TIMP2 and TIMP1 genes (2 cases), and MMP9 gene was found to be methylated in 91.2 % (31 cases). CONCLUSION: In our pilot study, we found no association between DNA methylation of gelatinases and their tissue inhibitors, and the development of an abdominal aortic aneurysm (Tab. 2, Fig. 1, Ref. 27).


Subject(s)
Aortic Aneurysm, Abdominal , DNA Methylation , Matrix Metalloproteinase 2 , Matrix Metalloproteinase 9 , Tissue Inhibitor of Metalloproteinase-1 , Tissue Inhibitor of Metalloproteinase-2 , Aortic Aneurysm, Abdominal/genetics , Humans , Male , Matrix Metalloproteinase 2/genetics , Matrix Metalloproteinase 2/metabolism , Matrix Metalloproteinase 9/genetics , Matrix Metalloproteinase 9/metabolism , Matrix Metalloproteinases , Pilot Projects , Tissue Inhibitor of Metalloproteinase-1/genetics , Tissue Inhibitor of Metalloproteinase-1/metabolism , Tissue Inhibitor of Metalloproteinase-2/genetics , Tissue Inhibitor of Metalloproteinase-2/metabolism
6.
Adv Exp Med Biol ; 911: 23-32, 2016.
Article in English | MEDLINE | ID: mdl-26987327

ABSTRACT

Phase I enzymes, including cytochrome P450, family 1, subfamily A, and polypeptide 2 (CYP1A2), are involved in the activation of carcinogens to reactive intermediates that are capable of binding covalently to DNA to form DNA adducts, potentially initiating the carcinogenic process. The aim of present study was to investigate the association of CYP1A2 gene polymorphisms and haplotypes with lung cancer risk. A case-control study was carried out on 105 lung cancer patients and 189 controls. To investigate three CYP1A2 polymorphisms: rs2472299, rs2470890, rs11072508 we used a high resolution melting analysis. We found significant allele associations (rs2470890 and rs2422299) with lung cancer risk. We searched for meaningful associations for all variants in the dominant, recessive, and additive genetic models. Genotype associations in the recessive model were of marginal significance for the same single nucleotide polymorphisms. A haplotype analysis included five variants with the frequency higher than 1 %. The haplotype "acc", present with the highest frequency, was associated with increased lung cancer risk (38.7 % vs. 31.5 %; OR 1.38; 95 %CI 0.95-2.01). On the contrary, rare haplotype "gtc" was significantly associated with decreased lung cancer risk in the Slovak population. In conclusion, the present study identified the risk alleles and haploid genotype associations of the CYP1A2 gene in lung cancer.


Subject(s)
Cytochrome P-450 CYP1A2/genetics , Genetic Predisposition to Disease , Haplotypes/genetics , Lung Neoplasms/genetics , Polymorphism, Single Nucleotide/genetics , Aged , Aged, 80 and over , Case-Control Studies , Female , Follow-Up Studies , Genotype , Humans , Lung Neoplasms/pathology , Male , Middle Aged , Neoplasm Staging , Prognosis , Risk Factors
7.
Transpl Immunol ; 29(1-4): 130-7, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24051241

ABSTRACT

Interest in mixed chimerism has evolved from its role in the induction of alloantigen tolerance. However, its precise impact on the host organism remains to be elucidated. In the present work, we analyzed cytokine secretion from chimeric mice cells to assess the influence of different mixed chimerism induction protocols on immune system function in recipient mice. To our knowledge, there have been no reports on using this parameter for the optimization of the mixed chimerism induction method. B6.SJL-PtprcaPep3b or C57BL/6J mice were used as recipients and Balb/c as donors. We utilized four protocols which consisted of: 3Gy total body irradiation (day -1), the injection of 20-30×10(6) bone marrow cells (day 0), and a combination of CD40L (days 0 and 4), CD8 (day -2), and NK1.1 (day -3) blocking antibodies and cyclophosphamide (175mg/kg - day 2). The concentrations of cytokines (IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNF) were evaluated in the supernatants of unstimulated or phytohemagglutinin-stimulated chimeric spleen, bone marrow and peripheral blood cells in the 8th week of experiment. The induction of tolerance to Balb/c mouse antigens was initially tested in chimeric mice by assessing the presence of Vß5 and Vß11 TCR-expressing lymphocytes. The cytokine production was considerably increased, especially in chimeric mice treated by cyclophosphamide. Also the mixed chimerism itself seems to affect IFN-γ, IL-2, IL-4, IL-6, IL-17A, and TNF secretion. Using the optimized induction protocol, we established that chimeric mice cells secreted lower IFN-γ, IL-2, IL-4 and higher IL-6, IL-17A, and TNF levels as compared to control animals. We found that both donor and recipient cells markedly participated in the cytokine production. In conclusion, our optimization study based on cytokine assessment contributes to establishing an effective protocol of mixed chimerism induction with no cyclophosphamide use and better understanding of the influence of this phenomenon on the recipient organism.


Subject(s)
Bone Marrow Transplantation , Cytokines/immunology , Receptors, Antigen, T-Cell, alpha-beta/immunology , Transplantation Chimera/immunology , Transplantation Tolerance , Allografts , Animals , Cytokines/metabolism , Mice , Mice, Inbred BALB C , Mice, Transgenic , Receptors, Antigen, T-Cell, alpha-beta/metabolism , Transplantation Chimera/metabolism
8.
Transplant Proc ; 41(5): 1894-9, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19545752

ABSTRACT

Mixed chimerism has been suggested to produce allograft tolerance. Since this phenomenon is not fully understood, the aim of our study was to evaluate various protocols for chimerism induction in a mouse model. B6.SJL-Ptprc(a)Pep3(b) mice were injected with 20 to 30 x 10(6) bone marrow cells from Balb C mice. Conditioning consisted of total body gamma irradiation with 9.5, 5, and 3 Gy on "-1 day" of the experiment, with 200 mg/kg cyclophosphamide (CP) ("+2 day"). Additionally, one group of mice received blocking antibody against CD40L on days 0, 1, 4, and 7. The presence of mixed chimerism in peripheral blood was assessed at 1, 2, 3, 4, 6, and 8 weeks using flow cytometry to detect CD45.1 or CD45.2 antigen expression. Moreover, the chimerism was examined in CD4, CD8, CD45/B220, Mac-1alpha subpopulations in peripheral blood and bone marrow cells at 8 weeks. We also compared chimerism in peripheral blood, bone marrow, and spleen leukocyte populations. We observed that the most effective conditioning method with relatively low toxicity was based on concomitant use of 5 Gy total body irradiation and CP. The percentage of donor cells differed among peripheral blood subpopulations and bone marrow cells, but was similar in leukocyte populations derived from various sources. Our experiments sought to optimize the induction of stable mixed chimerism.


Subject(s)
Bone Marrow Transplantation/methods , Transplantation Chimera/physiology , Animals , CD40 Ligand/immunology , Cyclophosphamide/pharmacology , Gamma Rays , Immunosuppressive Agents/pharmacology , Leukocyte Common Antigens/immunology , Mice , Mice, Inbred BALB C
9.
Folia Histochem Cytobiol ; 47(3): 395-400, 2009 Jan.
Article in English | MEDLINE | ID: mdl-20164023

ABSTRACT

Studies on mixed chimerism are currently focused primarily on obtaining less toxic conditioning protocols. With these issues in mind, we have undertaken the attempt to optimize the procedure of mixed chimerism induction in mice. In order to reduce toxicity, we used decreasing doses of total body irradiation (TBI) together with combination of blocking antibodies. We also tried to eliminate immunosuppression (cyclophosphamide - CP) treatment after bone marrow transplantation. B6.SJL-PtprcaPep3b mice were injected with 20-30 x 106 bone marrow cells from Balb C mice. Mice were treated with TBI (3 - 1.5 - 0 Gy) on "-1" day of the experiment and blocking antibodies against CD40L ("0", and "4" days) and additionally anti-CD8 ("-2" day) and/or anti-NK1.1 ("-3" day). Mice in certain groups also received CP (175 mg/kg) on "2" day. Presence of mixed chimerism was assessed in peripheral blood cells by flow cytometry on the 1st, 2nd, 3rd, 4th, 6th and 8th weeks of the experiment by detecting of CD45.1 (characteristic for B6.SJL-PtprcaPep3b strain) and CD45.2 (characteristic for Balb C strain) antigens expression. We also analyzed the percentage of peripheral blood CD8 T-cells (CD3e/CD8a) and NK cells (Ly-49D/NK1.1). We found that reduction of TBI dose and elimination of CP decrease the rate of mixed chimerism formation. The highest percentage of donor cells was obtained in the group of animals treated with 3 Gy of TBI, CP and combination of anti-CD40L, anti-CD8, and anti-NK1.1 antibodies. The 3 Gy TBI was necessary to induce stable mixed chimerism, but it could be obtained without the CP use. The percentage of CD3e/CD8a and Ly-49D/NK1.1 cells was significantly lower in the groups of mice treated by corresponding antibodies. Moreover, we observed the lowest number of peripheral blood Ly-49D/NK1.1 cells in the group of animals with highest mixed chimerism. Our experiments in mice model can help in better understanding of mixed chimerism phenomenon and in selecting the method of mixed chimerism induction with lowest possible toxicity. This also might improve the protocols of stable mixed chimerism induction in humans, and in the future, the effectiveness of vascularized organ transplantation.


Subject(s)
Models, Animal , Transplantation Chimera/immunology , Transplantation Conditioning/methods , Animals , Antibodies, Blocking/immunology , Bone Marrow Transplantation , CD8-Positive T-Lymphocytes/immunology , Cyclophosphamide/administration & dosage , Cyclophosphamide/immunology , Dose-Response Relationship, Radiation , Flow Cytometry , Killer Cells, Natural/immunology , Mice , Mice, Inbred BALB C , Radiation Dosage , Whole-Body Irradiation
10.
Folia Histochem Cytobiol ; 46(3): 299-305, 2008.
Article in English | MEDLINE | ID: mdl-19056533

ABSTRACT

The transplantation of hematopoietic stem and progenitor cells (HSPC) is an established lifesaving therapy. Bone marrow (BM), harvested from heparinized cadaveric organ donors, peripheral blood (PB) and cord blood (CB), are important sources of hematopoietic stem cells. HSPCs, which are used for transplantation purposes, are routinely evaluated in terms of number of mononuclear cells (MNCs), CD34+ MNCs count and viability. The efficacy of grafting is determined additionally in clonogenic tests in vitro. These tests deliver important information about the number of HSPCs and their proliferative potential. Unfortunately, they do not give a possibility to evaluate the functional HSPC chemotactic reactivity in the SDF-1 gradient, which is probably the key phenomenon for HSPC homing after transplantation procedure. Thus, the aim of our study was to optimize HSPC isolation according to their chemotactic reactivity in SDF-1 gradient. Using multiparameter cell sorter (FACS Aria, BD) we examined the HSPCs attracted by SDF-1 on a single cell level. The population of cells which participated in the chemotactic process was highly enriched in CXCR4+lin-AC133+CD45+ cells (referred as hematopoietic stem cells) and to our surprise in CXCR4+lin-AC133+CD45- cells (referred as pluripotent stem cells) in quantitative amounts. Since reactivity of HSPCs may depend on various factors involved in the protocol of their isolation and short-term storage, we tested the most commonly used anticoagulants (ACD, CPDA-1, EDTA and Heparin) and culture media (DME, IMDM, RPMI). HSPCs, harvested from CB, PB and BM, were subsequently investigated for clonogenic growth of CFU-GM in methylcellulose cultures and for the level of apoptosis by employing annexin V staining. Evaluating clonogenic potential, ability of chemotactic reactivity in SDF-1 gradient and intensification of apoptosis of HSPC as the most safe anticoagulant and medium were selected. This study has proved that chemotactic reactivity of HSPCs is a new but very important parameter which should be included in the procedure of their isolation.


Subject(s)
Cell Separation/methods , Chemotaxis , Hematopoietic Stem Cell Transplantation , Hematopoietic Stem Cells/physiology , Bone Marrow Cells/cytology , Bone Marrow Cells/immunology , Bone Marrow Cells/metabolism , Cells, Cultured , Cord Blood Stem Cell Transplantation , Female , Graft Enhancement, Immunologic , Hematopoietic Stem Cells/cytology , Hematopoietic Stem Cells/immunology , Humans , Weights and Measures
11.
Leukemia ; 21(2): 297-303, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17136117

ABSTRACT

Recently, we purified from adult murine bone marrow (BM) a population of CXCR4(+), Oct-4(+) SSEA-1(+), Sca-1(+) lin(-) CD45(-) very small embryonic-like (VSEL) stem cells and hypothesized that similar cells could be also present in human cord blood (CB). Here, we report that by employing a novel two-step isolation procedure -- removal of erythrocytes by hypotonic lysis combined with multiparameter sorting -- we could isolate from CB a population of human cells that are similar to murine BM-derived VSELs, described previously by us. These CB-isolated VSELs (CB-VSEL) are very small (3-5 micro m) and highly enriched in a population of CXCR4(+)AC133(+)CD34(+)lin(-) CD45(-) CB mononuclear cells, possess large nuclei containing unorganized euchromatin and express nuclear embryonic transcription factors Oct-4 and Nanog and surface embryonic antigen SSEA-4. Further studies are needed to see if human CB-isolated VSELs similar to their murine BM-derived counterparts are endowed with pluripotent stem cell properties.


Subject(s)
Embryonic Stem Cells/cytology , Fetal Blood/cytology , Glycosphingolipids/genetics , Octamer Transcription Factor-3/genetics , Receptors, CXCR4/genetics , Animals , Bone Marrow Cells/cytology , Cell Size , Embryonic Stem Cells/ultrastructure , Humans , Infant, Newborn , Mice , Microscopy, Electron , RNA/genetics , RNA/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction , Stage-Specific Embryonic Antigens
12.
Transplant Proc ; 38(1): 16-9, 2006.
Article in English | MEDLINE | ID: mdl-16504653

ABSTRACT

We have attempted to evaluate the level of the earliest human hematopoietic cell marker expression (CD34, CD117, CD133, CD184) on cells obtained from heparinized cadaveric organ donors before and after disconnection from the respirator. Moreover, we compared various cell populations: (1) coexpressing CD34/CD117; (2) CD34/CD133; (3) highly enriched hematopoietic stem cells (CD34+CXCR4+CD45+); and (4) highly enriched tissue-committed stem cells (CD34+CXCR4+CD45-). Finally, we analyzed whether the level of hematopoietic stem cell marker expression depended on the age of the donor. The expression of the membrane receptors (CD34, CD45, CD117, CD133, CD184) was studied by flow cytometry. We observed that the proportion of mononuclear cells expressing these markers slightly decreased in bone marrow harvested after disconnection from the respirator compared with the samples obtained before disconnection. Moreover, the proportion of cells expressing CD117 antigen depended on age of the donor.


Subject(s)
Leukocytes, Mononuclear/physiology , Stem Cells/physiology , Tissue Donors , Ventilators, Mechanical , Antigens, CD/analysis , Biomarkers/analysis , Flow Cytometry , Hematopoietic Stem Cells/physiology , Humans , Tissue and Organ Harvesting/methods
13.
Neoplasma ; 52(4): 287-91, 2005.
Article in English | MEDLINE | ID: mdl-16059643

ABSTRACT

The increased occurrence of lung cancer in residents of Dolny Kubin, the North-Slovakia district with ferrochromium industry, compared to the general population of Slovakia, led us to the study assessing influence of the occupational and environmantal exposure to chromium on the lung cancer incidence, respecting also the risk coming from cigarette smoking. Residents of Dolny Kubin district with the diagnosed lung cancer in 1984-1999 were involved in the study. The occurrence of lung cancer was significantly higher in people working in ferrochromium industry. The age at the onset of the disease in people exposed to chromium was by 5.5 years lower than in non-exposed. Smoking was an important risk factor, which has been proved particularly in non-exposed group where 62% were smokers and the onset of the lung cancer in them occured about 3.4 years earlier than in non-smokers. In exposed groups, no significant effect of smoking was found. We can conclude, that occupational exposure to chromium was identified as the main risk factor of lung cancer in Dolny Kubin district even overlaying effect of smoking.


Subject(s)
Chromium/poisoning , Environmental Exposure , Lung Neoplasms/epidemiology , Lung Neoplasms/etiology , Occupational Exposure , Smoking/adverse effects , Female , Humans , Incidence , Industry , Male , Risk Assessment , Slovakia/epidemiology
14.
J Investig Allergol Clin Immunol ; 14(2): 149-53, 2004.
Article in English | MEDLINE | ID: mdl-15301305

ABSTRACT

Bacterial flora of various strains was isolated from sputum and in some cases from BAL fluid of 75 patients with obstructive pulmonary disease experiencing dyspnea symptoms accompanying infections of respiratory tract. Among strains recognized traditionally as pathogenic in respiratory tract, we also isolated various strains typically called "normal oropharyngeal flora", "physiologic" or "non-pathogenic" bacteria. Those latter strains used in the skin tests in autologous manner for each patient had the property of provoking early (15 min) and late (24-48h) reactions. Early reactivity resembles that induced in the tests with airborn allergens. This suggests a potentially important role of those currently ignored strains in pathogenesis of obstructive pulmonary disease.


Subject(s)
Gram-Negative Bacteria/immunology , Gram-Negative Bacterial Infections/microbiology , Gram-Positive Bacteria/immunology , Gram-Positive Bacterial Infections/microbiology , Pulmonary Disease, Chronic Obstructive/microbiology , Respiratory Tract Infections/microbiology , Adolescent , Adult , Aged , Bronchoalveolar Lavage Fluid/microbiology , Gram-Negative Bacteria/isolation & purification , Gram-Negative Bacterial Infections/immunology , Gram-Positive Bacteria/isolation & purification , Gram-Positive Bacterial Infections/immunology , Humans , Middle Aged , Pulmonary Disease, Chronic Obstructive/immunology , Respiratory Tract Infections/immunology , Skin Tests , Sputum/microbiology
15.
Folia Histochem Cytobiol ; 35(2): 63-8, 1997.
Article in English | MEDLINE | ID: mdl-9151079

ABSTRACT

Different inflammatory cytokines are involved in the pathogenesis of the anemia of chronic disease (ACD), by inhibiting the proliferation of erythroid progenitors. Leukemia inhibitory factor (LIF), is an important inflammatory cytokine, and the purpose of this study was to evaluate its effect on the proliferation and viability of human erythroid progenitors. We have found that LIF slightly increased the survival of human early progenitor cells cultured under serum free conditions. LIF also slightly costimulated in vitro growth of human erythroid colonies. This last effect seems to be a direct one, because we found that LIF receptor (LIF-R) is expressed in cells isolated from growing in vitro human erythroid BFU-E colonies. These data, and the data reported by others in in vivo models, where LIF administration to experimental animals did not change the values of erythropoietic parameters, demonstrate that this inflammatory cytokine itself is not involved in the pathogenesis of ACD.


Subject(s)
Erythroid Precursor Cells/physiology , Growth Inhibitors/physiology , Interleukin-6 , Lymphokines/physiology , Antigens, CD34/analysis , Blood , Cell Division , Cell Survival , Cells, Cultured , Clone Cells/metabolism , Erythroid Precursor Cells/cytology , Flow Cytometry , Hematopoiesis , Humans , Leukemia Inhibitory Factor , Leukemia Inhibitory Factor Receptor alpha Subunit , Receptors, Cytokine/metabolism , Receptors, OSM-LIF , Recombinant Proteins/metabolism
16.
Acta Haematol Pol ; 26(2): 171-7, 1995.
Article in Polish | MEDLINE | ID: mdl-7653222

ABSTRACT

The influence of macrophage inflammatory protein-1 alpha (MIP-1 alpha) on cloning efficiency of human erythroid progenitors was evaluated. In our hands, in performed experiments in serum supplemented as in serum free cloning culture systems we did not observe any effect of MIP-1 alpha on erythroid colony formation by human CD34+ bone marrow cells. Therefore, our data did not confirm the possibility of the direct role of MIP-1 alpha in the pathogenesis of the anaemia of chronic disease.


Subject(s)
Cytokines/physiology , Erythroid Precursor Cells/physiology , Erythropoiesis/physiology , Monokines/physiology , Bone Marrow/physiology , Chemokine CCL4 , Clone Cells , Humans , Macrophage Inflammatory Proteins
17.
Virology ; 98(2): 489-92, 1979 Oct 30.
Article in English | MEDLINE | ID: mdl-18631617

ABSTRACT

Exposure of tobacco (Tb) and tomato (Tm) isolates of tobacco mosaic virus (TMV) to dilute alkaline solutions (pH > 8.0) at 0 degrees results in disassembly of the virus particles. Over the range of pH and NaCl concentration studied, Tm-TMV was more sensitive to alkaline conditions than was Tb-TMV. Kinetic analysis demonstrates that both isolates disassemble in a stepwise manner and that each produces six major intermediate-size particles.

18.
Virology ; 97(2): 488-91, 1979 Sep.
Article in English | MEDLINE | ID: mdl-18631607

ABSTRACT

Controlled alkaline disassembly of tobacco mosaic virus (TMV) results in the production of two populations of partially stripped virus. These particles have been designated PSV5 and 6 and are the two smallest particles produced during alkaline disassembly of TMV. The RNA from these particles was able to reassemble with TMV coat protein. The reassembled particles were resistant to ribonuclease treatment and sedimented upon sucrose density gradient centrifugation to the same position as authentic PSV5 and 6 particles.

19.
J Virol ; 29(2): 431-7, 1979 Feb.
Article in English | MEDLINE | ID: mdl-16789173

ABSTRACT

Kinetic analysis of the alkaline degradation of tobacco mosaic virus revealed degradation to be a stepwise process during which five distinct intermediate nucleoprotein particles were formed. Each intermediate accumulated before being degraded to the next smaller particle. After prolonged exposure to alkali, a stable nucleoprotein particle accumulated. The rate of alkaline degradation of tobacco mosaic virus was retarded by lowering pH (10.3 to 9.0), increasing salt concentration (0 to 100 mM), or increasing incubation temperature over the range of 0 to 22 degrees C. A fraction (15 to 25%) of the total virus population appeared to be completely resistant to alkaline degradation; however, the progeny of this virus fraction was sensitive to alkaline degradation.

20.
Vet Med (Praha) ; 23(10): 633-40, 1978 Oct.
Article in Slovak | MEDLINE | ID: mdl-102071

ABSTRACT

Five hundred and thirty-six samples of honeycombs were examined in a laboratory in the years 1971-1974. In all the samples clinically determined as the foul brood, B. alvei was isolated as a pure culture, and enterococci, or both microorganisms were isolated in mixed form. Twenty-five strains of the isolated streptococci were analyzed microbiologically and biochemically; on the basis of their culture and biochemical characteristics five strains were designated as Streptococcus faecalis, 14 strains as Streptococcus faecalis var. liquefaciens, five strains as Streptococcus faecium and one strain as Streptococcus durans. After checking the used taxonomic key of the culture and biochemical classification of B. alvei it may be stated that the culture and biochemical characteristics are stable. The strains of B. alvei (very dried strains), which persisted in the dried slant meat-peptone agar, were viable under the laboratory conditions, which proves the high resistance of the spores to the environment.


Subject(s)
Bacillus/isolation & purification , Bees/microbiology , Streptococcal Infections/veterinary , Streptococcus/isolation & purification , Animals , Bacillus/classification , Czechoslovakia , Enterococcus faecalis/isolation & purification , Species Specificity , Streptococcus/classification
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