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Multiple domains mediate heme control of the yeast activator HAP1.

Haldi, M L; Guarente, L.

Mol Gen Genet ; 248(2): 229-35, 1995 Jul 28.

Article in English | MEDLINE | ID: mdl-7651346

ABSTRACT

The activity of the yeast activator HAP1 in vivo requires heme. A heme responsive domain of HAP1 was identified previously. It is adjacent to the DNA-binding domain and appears to block DNA binding in the absence of heme. Here we describe a novel genetic selection which yielded mutants of HAP1 that are independent of heme when assayed for activation. These mutants define a second region of HAP1, close to the activation domain, which also controls its response to heme.

Subject(s)

DNA, Fungal/metabolism , DNA-Binding Proteins/metabolism , Fungal Proteins/metabolism , Heme/pharmacology , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Trans-Activators/metabolism , 5-Aminolevulinate Synthetase/genetics , Amino Acid Sequence , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/genetics , Fungal Proteins/chemistry , Fungal Proteins/genetics , Gene Expression Regulation, Fungal/genetics , Heme/metabolism , Molecular Sequence Data , Mutagenesis , Trans-Activators/chemistry , Trans-Activators/genetics , Transcription Factors , Transcriptional Activation

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