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1.
J Biol Chem ; 276(42): 39067-75, 2001 Oct 19.
Article in English | MEDLINE | ID: mdl-11495908

ABSTRACT

Rab11a is a small GTP-binding protein enriched in the pericentriolar plasma membrane recycling systems. We hypothesized that Rab11a-binding proteins exist as downstream effectors of its action. Here we define a family of four Rab11-interacting proteins: Rab11-Family Interacting Protein 1 (Rab11-FIP1), Rab11-Family Interacting Protein 2 (Rab11-FIP2), Rab11-Family Interacting Protein 3 (Rab11-FIP3), and pp75/Rip11. All four interacting proteins associated with wild type Rab11a and dominant active Rab11a (Rab11aS20V) as well as Rab11b and Rab25. Rab11-FIP2 also interacted with dominant negative Rab11a (Rab11aS25N) and the tail of myosin Vb. The binding of Rab11-FIP1, Rab11-FIP2, and Rab11-FIP3 to Rab11a was dependent upon a conserved carboxyl-terminal amphipathic alpha-helix. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 colocalized with Rab11a in plasma membrane recycling systems in both non-polarized HeLa cells and polarized Madin-Darby canine kidney cells. GFP-Rab11-FIP3 also colocalized with Rab11a in HeLa cells. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 also coenriched with Rab11a and H(+)K(+)-ATPase on parietal cell tubulovesicles, and Rab11-FIP1 and Rab11-FIP2 translocated with Rab11a and the H(+)K(+)-ATPase upon stimulating parietal cells with histamine. The results suggest that the function of Rab11a in plasma membrane recycling systems is dependent upon a compendium of protein effectors.


Subject(s)
rab GTP-Binding Proteins/chemistry , rab GTP-Binding Proteins/metabolism , Amino Acid Sequence , Animals , Antineoplastic Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Base Sequence , Blotting, Western , Cell Line , Cell Membrane/metabolism , Cloning, Molecular , Conserved Sequence , DNA, Complementary/metabolism , Dogs , Expressed Sequence Tags , Gastric Mucosa/metabolism , Gene Deletion , Gene Library , Genes, Dominant , HeLa Cells , Histamine/metabolism , Humans , Microscopy, Fluorescence , Molecular Sequence Data , Nocodazole/pharmacology , Paclitaxel/pharmacology , Protein Binding , Rabbits , Recombinant Proteins/metabolism , Sequence Homology, Amino Acid , Two-Hybrid System Techniques
2.
J Biol Chem ; 276(42): 38645-51, 2001 Oct 19.
Article in English | MEDLINE | ID: mdl-11509571

ABSTRACT

Disruption of the survival motor neuron (SMN) gene leads to selective loss of spinal motor neurons, resulting in the fatal human neurodegenerative disorder spinal muscular atrophy (SMA). SMN has been shown to function in spliceosomal small nuclear ribonucleoprotein (snRNP) biogenesis and pre-mRNA splicing. We have demonstrated that SMN also interacts with fibrillarin, a highly conserved nucleolar protein that is associated with all Box C/D small nucleolar RNAs and functions in processing and modification of rRNA. Fibrillarin and SMN co-immunoprecipitate from HeLa cell extracts indicating that the proteins exist as a complex in vivo. Furthermore, in vitro binding studies indicate that the interaction between SMN and fibrillarin is direct and salt-stable. We show that the glycine/arginine-rich domain of fibrillarin is necessary and sufficient for SMN binding and that the region of SMN encoded by exon 3, including the Tudor domain, mediates the binding of fibrillarin. Tudor domain missense mutations, including one found in an SMA patient, impair the interaction between SMN and fibrillarin (as well as the common snRNP protein SmB). Our results suggest a function for SMN in small nucleolar RNP biogenesis (akin to its known role as an snRNP assembly factor) and reveal a potential link between small nucleolar RNP biogenesis and SMA.


Subject(s)
Chromosomal Proteins, Non-Histone/chemistry , Chromosomal Proteins, Non-Histone/metabolism , Nerve Tissue Proteins/chemistry , Nerve Tissue Proteins/metabolism , Amino Acid Sequence , Animals , Arginine/chemistry , Cell Nucleolus/metabolism , Chromosomal Proteins, Non-Histone/genetics , Cyclic AMP Response Element-Binding Protein , DNA, Complementary/metabolism , Exons , Gene Library , Glycine/chemistry , HeLa Cells , Humans , Molecular Sequence Data , Mutation, Missense , Nerve Tissue Proteins/genetics , Precipitin Tests , Protein Binding , Protein Structure, Tertiary , RNA-Binding Proteins , SMN Complex Proteins , Two-Hybrid System Techniques , Xenopus
3.
Mol Biol Cell ; 12(6): 1843-57, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11408590

ABSTRACT

Myosin Va is associated with discrete vesicle populations in a number of cell types, but little is known of the function of myosin Vb. Yeast two-hybrid screening of a rabbit parietal cell cDNA library with dominant active Rab11a (Rab11aS20V) identified myosin Vb as an interacting protein for Rab11a, a marker for plasma membrane recycling systems. The isolated clone, corresponding to the carboxyl terminal 60 kDa of the myosin Vb tail, interacted with all members of the Rab11 family (Rab11a, Rab11b, and Rab25). GFP-myosin Vb and endogenous myosin Vb immunoreactivity codistributed with Rab11a in HeLa and Madin-Darby canine kidney (MDCK) cells. As with Rab11a in MDCK cells, the myosin Vb immunoreactivity was dispersed with nocodazole treatment and relocated to the apical corners of cells with taxol treatment. A green fluorescent protein (GFP)-myosin Vb tail chimera overexpressed in HeLa cells retarded transferrin recycling and caused accumulation of transferrin and the transferrin receptor in pericentrosomal vesicles. Expression of the myosin Vb tail chimera in polarized MDCK cells stably expressing the polymeric IgA receptor caused accumulation of basolaterally endocytosed polymeric IgA and the polymeric IgA receptor in the pericentrosomal region. The myosin Vb tail had no effects on transferrin trafficking in polarized MDCK cells. The GFP-myosin Va tail did not colocalize with Rab11a and had no effects on recycling system vesicle distribution in either HeLa or MDCK cells. The results indicate myosin Vb is associated with the plasma membrane recycling system in nonpolarized cells and the apical recycling system in polarized cells. The dominant negative effects of the myosin Vb tail chimera indicate that this unconventional myosin is required for transit out of plasma membrane recycling systems.


Subject(s)
Cell Membrane/chemistry , Cell Membrane/metabolism , Myosins/chemistry , Myosins/metabolism , Amino Acid Sequence , Animals , Biological Transport , DNA, Complementary/metabolism , Dogs , Gene Library , Green Fluorescent Proteins , HeLa Cells , Humans , Luminescent Proteins/metabolism , Molecular Sequence Data , Mutation , Protein Binding , Protein Structure, Tertiary , Rabbits , Receptors, Fc/metabolism , Recombinant Fusion Proteins/metabolism , Sequence Homology, Amino Acid , Time Factors , Transfection , Transferrin/chemistry , Transferrin/metabolism , Two-Hybrid System Techniques , rab GTP-Binding Proteins/chemistry , rab GTP-Binding Proteins/metabolism
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