Anticancer, Antioxidant, and Antibacterial Activities of the Methanolic Extract from Sphagneticola trilobata (L.) J. F Pruski Leaves.

This study aims to investigate the potential of bioactive secondary metabolites contained in Sphagneticola trilobata (L.) J.F Pruski leaves as novel plant-derived anticancer agent. Qualitative bioactive compound contents in the methanolic extract of S. trilobata leaves were screened using phytochemical method. Antioxidant evaluation was carried out using 2,2-diphenyl-1-picrylhydrazyl assay; antibacterial - using well diffusion method on Escherichia coli and Salmonella typhi; and cytotoxicity - using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on MCF-7 cell line and Vero Cell. It was found that the methanolic extract exhibited antioxidant activity with an IC50 value of 124.34 µg/mL. The inhibition zone values against E. coli and S. thypi (at extract concentration of 100 mg/mL) were 34.33 and 36 mm, respectively. In vitro MTT assay showed that our extract successfully reached 96% mortality with LC50 = 189.287 µg/mL, where the selective index of 2.5 suggest its selectivity against MCF-7 breast cancer cell line. In conclusion, the data of biological activities suggest the potential development of methanolic extract from S. trilobata leaves as a phytomedicine for breast cancer treatment.

Antioxidant and cytotoxic activities of the ethyl acetate extract of Sphagneticola trilobata (L.) J.F. Pruski on MCF-7 breast cancer cell.

Sphagneticola trilobata (L.) J.F. Pruski is the perennial herb distributed at tropical temperature. In this study, the antioxidant and anticancer properties of the ethyl acetate extract from S. trilobata leaves were investigated against MCF-7 breast cancer cells. The antioxidant and anticancer activities were assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay methods, respectively. The extract demonstrated DPPH free radical scavenging effect with IC50 value equaling to 127.43 µg/mL. The cytotoxic study was conducted on the concentration range of 1-200 µg/mL, and the results exhibited the strongest inhibitory activity against MCF-7 with the LC50 value of 58.143 µg/mL. The cytotoxic activity of the extract was supported by the induction of apoptosis cell which possessed the apoptosis percentage of 78.80%. Thus, the cheap herbal drug treatment might highly be recommended to treat effectively breast cancers as an ideal choice or combinational therapy.