1.
Appl Biochem Biotechnol
; 77-79: 827-34, 1999.
Article
in English
| MEDLINE
| ID: mdl-15304701
ABSTRACT
Halomonas (Deleya) sp. alpha-15 produces new co-factor binding soluble glucose 3-dehydrogenase (G3DH), which oxidizes the third hydroxy group of pyranose. This study investigated the condition of efficient production of G3DH using Halomonas (Deleya) sp. alpha-15. This enzyme was inducible, and alpha-methyl-D-glucoside, isopropyl-thiogalactopyranoside (IPTG) and lactose were revealed to be suitable carbon sources for G3DH induction. Maximum G3DH production was achieved by using minimal medium containing 0.8% (w/v) lactose with a productivity of 470U/l.