ABSTRACT
Solid lipid extrudates with the model drug praziquantel were produced with chemically diverse lipids and investigated regarding their dissolution behaviour in different media. The lipids used in this study were glyceryl tripalmitate, glyceryl dibehenate, glyceryl monostearate, cetyl palmitate and solid paraffin. Thermoanalytical and dissolution behaviour was investigated directly after extrusion and after 3 and 6 months open storage at 40°C/75% RH. Dissolution studies were conducted in hydrochloric acid (HCl) pH 1.2 with different levels of polysorbate 20 and with a biorelevant medium containing pancreatic lipase, bile salts and phospholipids. Furthermore, the impact of lipid digestion on drug release was studied using in vitro lipolysis. The release of praziquantel from cetyl palmitate and glyceryl monostearate in the biorelevant medium was much faster than in HCl, whereas there was hardly any difference for the other lipids. It was shown that drug release from glyceryl monostearate matrices is driven by both solubilisation and enzymatic degradation of the lipid, whereas dissolution from cetyl palmitate extrudates is dependent only on solubilisation by surfactants in the medium. Moreover, storage influenced the appearance of the extrudate surface and the dissolution rate for all lipids except solid paraffin.
Subject(s)
Anthelmintics/chemistry , Intestinal Secretions/chemistry , Lipids/chemistry , Praziquantel/chemistry , Bile Acids and Salts/chemistry , Chemistry, Pharmaceutical , Drug Storage , Glycerides/chemistry , Humidity , Hydrochloric Acid/chemistry , Hydrogen-Ion Concentration , Kinetics , Lipase/chemistry , Lipolysis , Palmitates/chemistry , Paraffin/chemistry , Phospholipids/chemistry , Polysorbates/chemistry , Solubility , Surface Properties , Technology, Pharmaceutical/methods , Temperature , Time Factors , Triglycerides/chemistryABSTRACT
The GPS recorder consists of a GPS receiver board, a logging facility, an antenna, a power supply, a DC-DC converter and a casing. Currently, it has a weight of 33 g. The recorder works reliably with a sampling rate of 1/s and with an operation time of about 3 h, providing time-indexed data on geographic positions and ground speed. The data are downloaded when the animal is recaptured. Prototypes were tested on homing pigeons. The records of complete flight paths with surprising details illustrate the potential of this new method that can be used on a variety of medium-sized and large vertebrates.
Subject(s)
Columbidae , Flight, Animal/physiology , Aircraft , Animals , Satellite CommunicationsSubject(s)
Surgical Procedures, Operative/mortality , Aged , Aged, 80 and over , Breast Neoplasms/surgery , Cause of Death , Female , Gastrointestinal Diseases/surgery , Germany, East , Hernia, Inguinal/surgery , Humans , Male , Postoperative Complications/mortality , Risk Factors , Wounds and Injuries/surgerySubject(s)
Retroperitoneal Neoplasms/diagnosis , Adolescent , Adult , Aged , Child , Diagnosis, Differential , Female , Humans , Lymphatic Metastasis , Male , Middle Aged , Retroperitoneal Neoplasms/surgerySubject(s)
Esophagus/injuries , Adult , Aged , Esophageal Diseases/diagnosis , Esophageal Diseases/surgery , Esophageal Perforation/diagnosis , Esophageal Perforation/surgery , Esophagus/surgery , Female , Foreign Bodies/diagnosis , Foreign Bodies/surgery , Humans , Iatrogenic Disease , Male , Middle Aged , Rupture, SpontaneousSubject(s)
Aneurysm/pathology , Hepatic Artery/pathology , Marfan Syndrome/complications , Adolescent , Diverticulitis, Colonic/surgery , Humans , Intestinal Perforation/surgery , Male , Marfan Syndrome/pathology , Postoperative Complications/pathology , Rupture, Spontaneous , Sigmoid Diseases/surgeryABSTRACT
Blood of pigs and cows from Langford/Bristol (U.K.) and Hannover (F.R.G.) used in membrane feeding of tsetse flies was analysed for 21 trace elements; an influence of the location of origin was not detectable. Between the two host species a significant difference of the element content was established for bromine, copper, rubidium, and zinc; if this observation is responsible for the different nutritive value of pig's and cow's blood it cannot be identified from the present experiment.
Subject(s)
Trace Elements/blood , Tsetse Flies/physiology , Animals , Cattle , Membranes, Artificial , SwineSubject(s)
Intestinal Neoplasms/surgery , Malignant Carcinoid Syndrome/surgery , Adult , Aged , Appendiceal Neoplasms/surgery , Female , Follow-Up Studies , Humans , Ileocecal Valve , Ileum , Intestinal Neoplasms/diagnosis , Male , Malignant Carcinoid Syndrome/diagnosis , Middle Aged , Rectal Neoplasms/surgerySubject(s)
Cholecystitis/surgery , Wounds and Injuries/complications , Accidents, Traffic , Adolescent , Humans , Male , Middle AgedABSTRACT
Among the various methods for studying the relative effects of transmutation and radiation of incorporated nuclides, simulation of beta radiation by external gamma exposure is of practical importance. Self-irradiation and mutual irradiation of the labeled cells cannot be neglected in any case. Furthermore, additional hypothetical and experimental problems may arise from using either external beta radiation or different isotopes of an element. By means of external gamma irradiation on the other hand, this being equivalent to the internal beta radiation from a microdosimetrical point of view, the radiation effect of the nuclide alone can be observed without any modification of other experimental parameters. To determine such equivalent gamma radiation for labeled cell nuclei of Vicia faba roots, the authors applied the Monte Carlo Method to the beta spectra of 32-P, 3-H, 14-C and 131-J, to the energy-dependent LET and to different cell diameters. The existence of secondary particle equilibrium inside the nuclei during gamma exposure was assumed. For certain radionuclides and cell sizes it is possible to calculate gamma spectra which induce energy spectra in the nuclei similar to those caused by the beta particles originating in the nuclear DNA.
