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1.
J Vet Intern Med ; 15(6): 585-8, 2001.
Article in English | MEDLINE | ID: mdl-11817065

ABSTRACT

We compared serum concentrations of zinc, chromium, and iron in dogs with cancer to those of normal dogs. Dogs with lymphoma (n = 50) and osteosarcoma (n = 52) were evaluated. Dogs with lymphoma had significantly lower (P = .0028) mean serum zinc concentrations (mean +/- SD; 1.0 +/- 0.3 mg/L) when compared to normal dogs (1.2 +/- 0.4 mg/L). Dogs with osteosarcoma also had lower mean serum zinc concentrations (1.1 +/- 0.4 mg/L), but this difference was not significant (P = .075). Serum chromium concentrations were significantly lower in dogs with lymphoma (2.6 +/- 2.6 microg/L, P = .0007) and osteosarcoma (2.4 +/- 3.1 microg/L, P = .0001) compared to normal dogs (4.7 +/- 2.8 microg/L). Serum iron concentrations and total iron-binding capacity were significantly lower in dogs with lymphoma (110.8 +/- 56.7 microg/dL, P < .0001, and 236.6 +/- 45.6 microg/dL, P < .0001, respectively) and osteosarcoma (99.6 +/- 49.3 microg/dL, P < .0001, and 245.0 +/- 43.8 microg/dL, P = .0011, respectively) when compared to normal dogs (175.1 +/- 56.7 microg/dL and 277.1 +/- 47.4 microg/dL). Mean ferritin concentration was significantly higher in dogs with lymphoma (1291.7 +/- 63.0 microg/L) than in normal dogs (805.8 +/- 291.1 microg/L, P < .0001) and dogs with osteosarcoma (826.5 +/- 309.2 microg/L, P < .0001). Further investigation is needed to explore the clinical significance of these mineral abnormalities in dogs with cancer.


Subject(s)
Bone Neoplasms/veterinary , Chromium/blood , Dog Diseases/pathology , Iron/blood , Lymphoma/veterinary , Osteosarcoma/veterinary , Zinc/blood , Animals , Bone Neoplasms/pathology , Case-Control Studies , Chromium/deficiency , Dogs , Female , Glucose Tolerance Test/veterinary , Hyperinsulinism/veterinary , Killer Cells, Natural , Lymphoma/pathology , Male , Osteosarcoma/pathology , Zinc/deficiency
2.
Am J Vet Res ; 61(4): 450-5, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10772113

ABSTRACT

OBJECTIVE: To determine and compare substrate specificity and kinetic rate constants of feline and canine alcohol dehydrogenase (ADH) with ethanol (EtOH) and ethylene glycol (EG) as substrates in vitro, with and without fomepizole. SAMPLE POPULATION: Livers from 3 dogs and 3 cats. PROCEDURE: Canine and feline ADH activity, in cytosolic fractions of homogenized liver, was determined by use of various concentrations of nicotinamide adenine dinucleotide (NAD), EtOH, or EG as substrates. Initial reaction velocities were calculated, and kinetic inhibition rate constants (Ki) for fomepizole were determined. RESULTS: Substrate specificity of canine and feline ADH for EtOH or EG was not significantly different. A 2-fold difference was detected in the maximal velocity of canine, compared with feline, ADH, using either substrate. Fomepizole Ki in feline hepatic homogenates was significantly greater than Ki in canine hepatic homogenates when either EtOH or EG was used as substrate (10- and 30-fold, respectively). A 6-fold increase in the concentration of fomepizole was required to achieve ADH inhibition, with feline homogenates equivalent to those of canine homogenates. CONCLUSIONS AND CLINICAL RELEVANCE: Feline ADH has lower enzymatic capacity for turnover or is less concentrated in liver than canine ADH with regard to EtOH and EG catalysis. Canine ADH was more effectively inhibited by fomepizole than feline ADH. Results suggest that higher dosages of fomepizole may be more effective to treat cats with EG intoxication than dosages reported to treat dogs.


Subject(s)
Alcohol Dehydrogenase/metabolism , Antidotes/pharmacology , Cats/metabolism , Dogs/metabolism , Pyrazoles/pharmacology , Animals , Fomepizole , Kinetics , Liver/drug effects , Liver/enzymology , Substrate Specificity
3.
Am J Vet Res ; 60(3): 328-33, 1999 Mar.
Article in English | MEDLINE | ID: mdl-10188815

ABSTRACT

OBJECTIVE: To determine effects of dietary cysteine on blood sulfur amino acids (SAA), reduced glutathione (GSH), oxidized glutathione (GSSG), and malondialdehyde (MDA) concentrations in cats. ANIMALS: 12 healthy adult cats. PROCEDURE: Cats were fed diets with a nominal (0.50 g/100 g dry matter [DM]), moderate (1.00 g/100 g DM), or high (1.50 g/100 g DM) cysteine content in a 3 X 3 Latin square design with blocks of 8 weeks' duration. Venous blood samples were collected after each diet had been fed for 4 and 8 weeks, and a CBC and serum biochemical analyses were performed; poikilocyte, reticulocyte, and Heinz body counts were determined; and MDA, GSH, GSSG, and SAA concentrations were measured. RESULTS: Blood cysteine and MDA concentrations were not significantly affected by dietary cysteine content. Blood methionine, homocysteine, and GSSG concentrations were significantly increased when cats consumed the high cysteine content diet but not when they consumed the moderate cysteine content diet, compared with concentrations obtained when cats consumed the nominal cysteine content diet. Blood GSH concentrations were significantly increased when cats consumed the moderate or high cysteine content diet. CONCLUSIONS: Increased dietary cysteine content promotes higher blood methionine, homocysteine, GSH, and GSSG concentrations in healthy cats. CLINICAL RELEVANCE: Supplemental dietary cysteine may be indicated to promote glutathione synthesis and ameliorate adverse effects of oxidative damage induced by disease or drugs.


Subject(s)
Amino Acids/blood , Animal Nutritional Physiological Phenomena , Cats/blood , Cysteine/pharmacology , Glutathione/blood , Malondialdehyde/blood , Sulfur/blood , Animals , Critical Care , Dietary Supplements , Female , Male , Oxidative Stress
4.
J Am Vet Med Assoc ; 213(11): 1599-604, 1571, 1998 Dec 01.
Article in English | MEDLINE | ID: mdl-9838961

ABSTRACT

During a 2-week period, 16 of 150 recently weaned calves developed signs of polioencephalomalacia (PEM). One calf was examined and treated at our veterinary teaching hospital and a necropsy was performed on a calf that died. During the peak of the outbreak, clinicians visited the ranch. Ruminal hydrogen sulfide (H2S) and blood thiamine concentrations were measured in 10 clinically normal penmates of PEM-affected calves. Ruminal H2S concentrations were high (> 0.695 mg/L [> 500 ppm]) in all cattle (mean, 12.19 mg/L [8,770 ppm]). All blood thiamine values were within the reference range. Within 12 hours after measurement of blood thiamine concentrations, 2 of the calves from which samples were obtained developed clinical signs of PEM. Dietary analysis revealed an estimated sulfur intake of 0.9% per calf on a dry-matter basis. Hay contributed most of this sulfur. In the investigation reported here, an outbreak of PEM was associated with high ruminal H2S concentrations and excess sulfur intake without evidence of thiamine deficiency.


Subject(s)
Cattle Diseases/etiology , Encephalomalacia/veterinary , Hydrogen Sulfide/analysis , Rumen/chemistry , Sulfur/administration & dosage , Animal Feed/analysis , Animals , Brain/pathology , Cattle , Cattle Diseases/pathology , Encephalomalacia/etiology , Encephalomalacia/pathology , Female , Male , Muscle, Skeletal/pathology , Poaceae/chemistry , Sulfur/adverse effects , Sulfur/analysis , Water/analysis , Weaning
5.
Res Vet Sci ; 64(1): 11-6, 1998.
Article in English | MEDLINE | ID: mdl-9557799

ABSTRACT

Weight gain is a common problem in domestic cats, but little is known about its metabolic effects. The purpose of this study was to determine the effects of diet-induced weight gain and subsequent weight loss on metabolic rate, body composition, and glucose tolerance. Gain of approximately 20 per cent body weight (divided approximately equally between fat and fat-free mass) over three months resulted in insulin resistance in females, indicated by increases in basal insulin concentration (68.2+/-7.9 to 119+/-16.5 pmol litre(-1), P<0.05), insulin peak response to glucose (241.1+/-31.6 to 315.0+/-23.0 pmol litre(-1), P<0.05), and deltaI/deltaG (14.2+/-2.6 to 18.1+/-1.3 pmol mmol(-1), P<0.05) compared with pre-gain values. The same numerical trend was noted in male cats, however, changes were not significant (P>0.05). Alterations in serum lipids included significant (P<0.05) elevations in triglyceride concentrations in male cats and decreased beta-lipoprotein concentrations in both genders. Weight loss over three months normalised basal insulin, insulin response to glucose, and serum triglyceride concentrations, and resulted in significant (P<0.05) decreases in serum concentrations of beta- and prebeta-lipoproteins, cholesterol, and triiodothyronine. Diet-induced weight gain of three months' duration, followed by three months' maintenance of increased body weight did not affect fasting or resting metabolic rate. Development and severity of impaired glucose tolerance, insulin resistance, and other changes may be affected by duration and possibly severity of weight gain.


Subject(s)
Blood Glucose/metabolism , Cats/physiology , Lipids/blood , Weight Gain/physiology , Weight Loss/physiology , Adipose Tissue/anatomy & histology , Animals , Basal Metabolism , Body Composition , Cholesterol/blood , Energy Metabolism , Fasting , Female , Glucose Tolerance Test/veterinary , Insulin Resistance , Lipoproteins/blood , Male , Orchiectomy , Ovariectomy , Sex Characteristics , Thyroxine/blood , Time Factors , Triglycerides/blood , Triiodothyronine/blood
7.
Vet Pathol ; 34(5): 442-9, 1997 Sep.
Article in English | MEDLINE | ID: mdl-9381655

ABSTRACT

Transient echinocytosis has been reported in association with snake envenomation in humans and dogs. An in vitro model of echinocytosis induced by venom of crotalus atrox (western diamondback rattlesnake) was established to characterize erythrocyte morphologic changes and to investigate potential mechanisms of echinocytic transformation. Erythrocyte morphologic changes produced after the addition of venom to canine, feline, equine, and human blood were characterized by dose-dependent echinocytosis. Type III echinocytosis were consistently induced in vitro at a dose comparable to in vivo envenomation; higher venom doses produced spheroechinocytic and spherocytic transformations. The changes could not be induced in vitro in the presence of ethylenediaminetraacetic acid but were observed in heparinized and citrated blood samples, suggesting the participation of calcium or a metalloprotein in echinocytic change. These findings suggest that phospholipase A2 (PLA2), a calcium-dependent enzyme in snake venom, may be responsible for echinocytic transformation via the production of lysolecithin, a known echinocytic agent. Purified PLA2 from C. atrox venom induced dose-dependent echinocytic change in vitro in canine blood. Other potential mechanisms of echinocytic change evaluated in canine blood included erythrocyte cation loss and erythrocyte ATP depletion. In canine blood mixed with venom, erythrocyte sodium and potassium concentrations were consistently less than those of controls, likely as a result of membrane alteration produced by the actions of PLA2. There was no difference in blood ATP concentrations from dogs with snakebite when compared with normal controls; however, the power of this comparison was low. Echinocytosis induced by rattlesnake venom is related to the degree of venom exposure and may correlate clinically with the amount of venom absorbed. Echinocytic transformation in vitro is induced by PLA2 present in venom.


Subject(s)
Crotalid Venoms/pharmacology , Crotalus/metabolism , Dogs/blood , Erythrocytes/drug effects , Adenosine Triphosphate/analysis , Adenosine Triphosphate/blood , Animals , Calcium/physiology , Cats , Cell Membrane/drug effects , Cell Membrane/ultrastructure , Crotalid Venoms/chemistry , Crotalid Venoms/metabolism , Dose-Response Relationship, Drug , Erythrocytes/chemistry , Erythrocytes/ultrastructure , Horses , Humans , Hydrogen-Ion Concentration , Metalloproteins/physiology , Microscopy, Electron/methods , Microscopy, Electron/veterinary , Phospholipases A/analysis , Phospholipases A/pharmacology , Phospholipases A2 , Potassium/analysis , Sodium/analysis
8.
Res Vet Sci ; 62(2): 131-6, 1997.
Article in English | MEDLINE | ID: mdl-9243711

ABSTRACT

Few controlled studies have been made of the possible mechanisms and physiological consequences of weight gain after cats have been neutered. In this study, six male and six female cats were gonadectomised and compared with five entire male and six entire female cats, before they were neutered and one and three months later. The neutered males gained significantly more weight (mean [SEM] per cent) than the entire males (30.2 [5.2] v 11.8 [2.3]) and the entire females gained 40.0 (7.3) v 16.1 (3.3) per cent, (P < 0.05). The castrated males gained more weight as fat than the sexually intact males (22.0 [3.3] v 8.8 [4.5] per cent, P < 0.05). There was a significant increase (P < 0.05) in daily food intake after neutering. Spayed females underwent a significant decrease in fasting metabolic rate (83.7 [5.5] v 67.2 [2.3] kcal/kg bodyweight0.75/day P < 0.05). Gonadectomy had minimal effects on serum thyroid hormone concentrations, the resting or fasting metabolic rates in males, or on indices of glucose tolerance.


Subject(s)
Body Weight/physiology , Cats/metabolism , Cats/physiology , Glucose/pharmacology , Orchiectomy/veterinary , Ovariectomy/veterinary , Adipose Tissue/metabolism , Adipose Tissue/physiology , Animals , Body Composition/physiology , Body Water/metabolism , Cats/surgery , Cholesterol/blood , Digestion/physiology , Dose-Response Relationship, Drug , Eating/physiology , Female , Glucose/metabolism , Glucose Tolerance Test/veterinary , Insulin/blood , Male , Orchiectomy/adverse effects , Orchiectomy/methods , Ovariectomy/adverse effects , Ovariectomy/methods , Thyroid Hormones/blood , Triglycerides/blood , Weight Gain/physiology
9.
J Vet Diagn Invest ; 9(1): 72-6, 1997 Jan.
Article in English | MEDLINE | ID: mdl-9087929

ABSTRACT

Two groups of 3 120-160-kg Holstein steers were fed a diet high in carbohydrate and low in long fiber and either with or without added sodium sulfate. Prior to and during the course of feeding the experimental diet, the concentrations of rumen hydrogen sulfide gas and rumen fluid sulfide were determined by a simple sulfide detector tube method and by sulfide-selective electrode, respectively. Other measurements included rumen fluid pH, blood creatine kinase, and blood sulfhemoglobin. Two of the 3 steers fed the high-sulfate diet developed signs and lesions of polioencephalomalacia. Clinical signs included episodic ataxia and blunted or absent menace reaction. Increased ruminal H2S gas concentrations occurred in all 3 steers consuming the diet with added sulfate. The onset of clinical signs coincided with the onset of elevated H2S concentrations. These increases were 40-60 times the values measured in the steers consuming the diet without added sulfate. In contrast, increases in rumen fluid sulfide concentrations usually rose to 4 times that of control steers. The steers fed an identical diet but without added sulfate exhibited no signs or lesions of polioencephalomalacia and no elevations of sulfide in rumen gas or fluid. All steers had a modest decrease in rumen fluid pH associated with the transition to the concentrate diet. No significant changes were observed in any of the blood measurements of any of the steers. An additional pair of steers was fed the experimental diet with or without added sulfate to compare the ruminal H2S gas concentrations estimated by H2S detector tubes with those estimated by a different method of analysis utilizing charcoal trapping of H2S, conversion to sulfate, and measurement of the sulfate. Both methods yielded comparable estimates of H2S concentration. Overall, these data indicate that changes in rumen gas cap H2S concentrations are larger than changes in rumen fluid sulfide concentration and the estimation of rumen gas cap H2S concentration may be a practical approach to detecting pathologic increases in ruminal H2S gas. This simple, rapid, minimally invasive method should be useful for estimating the H2S content of ruminal gas under field conditions.


Subject(s)
Animal Feed , Cattle Diseases , Encephalomalacia/veterinary , Rumen/metabolism , Sulfides/metabolism , Animals , Cattle , Dietary Carbohydrates , Dietary Fiber , Encephalomalacia/etiology , Encephalomalacia/metabolism , Gastrointestinal Contents , Hydrogen Sulfide/analysis , Male , Orchiectomy , Rumen/pathology , Sulfates , Time Factors
10.
J Am Vet Med Assoc ; 209(11): 1880-3, 1996 Dec 01.
Article in English | MEDLINE | ID: mdl-8944802

ABSTRACT

OBJECTIVE: To evaluate safety and efficacy of 4-methylpyrazole (4-MP) treatment in dogs and to determine clinical signs and outcome of, and clinicopathologic abnormalities in, dogs treated in early or late stages of ethylene glycol (EG) intoxication. DESIGN: Retrospective study. ANIMALS: 107 dogs. PROCEDURE: For dogs treated with 4-MP, 1 of 2 dosage regimens was usually used: 20 mg/kg of body weight, IV, initially, 15 mg/kg 17 hours later, and 5 mg/kg 25 and 36 hours after the initial dose, or 20 mg/kg, IV, initially, 15 mg/kg 12 and 24 hours later, and 5 mg/kg 36 hours after the initial dose. RESULTS: Neither adverse clinical signs nor clinicopathologic abnormalities were associated with the administration of 4-MP except in 1 dog, which developed tachypnea, gagging, excess salivation, and trembling after the second dose of 4-MP was given. Ethylene glycol intoxication was confirmed in 37 dogs. Of these, 21 were azotemic or became azotemic within 18 hours after admission, and only 1 of the 21 survived. All 16 dogs that did not become azotemic survived. Median time from EG ingestion to treatment with 4-MP was 5 hours (range, 2 to 8.5 hours) for dogs that were not azotemic at admission and 14.5 hours (range, 8.5 to 38 hours) for dogs that were azotemic at admission. CLINICAL IMPLICATIONS: 4-MP was a safe and effective treatment for EG intoxication when it was given before sufficient quantities of EG had been metabolized to induce renal failure. Dogs treated within 8 hours of EG ingestion had a good prognosis.


Subject(s)
Alcohol Dehydrogenase/antagonists & inhibitors , Dog Diseases/drug therapy , Enzyme Inhibitors/therapeutic use , Ethylene Glycols/poisoning , Pyrazoles/therapeutic use , Acid-Base Equilibrium , Animals , Dog Diseases/chemically induced , Dog Diseases/mortality , Dogs , Enzyme Inhibitors/adverse effects , Ethylene Glycol , Female , Fomepizole , Male , Poisoning/drug therapy , Poisoning/mortality , Poisoning/veterinary , Prognosis , Pyrazoles/adverse effects , Retrospective Studies , Uremia/chemically induced , Uremia/mortality , Uremia/veterinary
11.
J Vet Diagn Invest ; 7(4): 527-30, 1995 Oct.
Article in English | MEDLINE | ID: mdl-8580177

ABSTRACT

Twenty forage samples were collected and selected for variation in nitrate content. Each forage samples was analyzed 4 times by 4 different methods: diphenylamine spot plate, spectrophotometric, nitrate-selective electrode, and high-performance liquid chromatographic. Five feed extracts were spiked with 2 different amounts of nitrate and analyzed by each method. The spectrophotometric and nitrate-selective electrode had similar percent recoveries, which were close to 100%. The nitrate-selective electrode method had the least variation of the 4 methods. The diphenylamine spot plate method had the poorest average recovery, greatest variation, and was the least accurate. The average coefficients of variation for all samples within a method were 15%, 12%, 6.4%, and 16 for the diphenylamine spot plate, spectrophotometric, nitrate-selective electrode, and high-performance liquid chromatographic methods, respectively. The variation in the nitrate-selective electrode method was lower (P < 0.05) than the other methods. The results from this study suggest that the nitrate-selective electrode method is more accurate and precise than the other methods of analysis tested.


Subject(s)
Animal Feed/analysis , Nitrates/analysis , Poaceae , Animals , Cattle , Chromatography, High Pressure Liquid/methods , Colorimetry/methods , Diphenylamine , Electrochemistry/methods , Spectrophotometry/methods
12.
Am J Vet Res ; 56(10): 1384-9, 1995 Oct.
Article in English | MEDLINE | ID: mdl-8928959

ABSTRACT

To study their role in sulfate reduction, anaerobic bacteria were cultured from rumen fluid samples of cattle fed high-carbohydrate, short-fiber diets with and without added sulfate. The steers fed the diet with added sulfate developed polioencephalomalacia. Microbiological methods included colony type profiles, molybdate sensitivity, presence of desulfoviridin, sulfate reduction rates of pure and mixed cultures, and incubation time effects on sulfate reduction. Colony-type profiles indicated decreased diversity, but no relative change in numbers of sulfate-reducing bacteria in rumen fluid from cattle fed diets with and without added sulfate. Thirteen bacteria] isolates were selected for further study on the basis of colony type, sulfate-reducing activity, and growth in lactate, sulfate, and yeast extract media. Seven of the isolates had Desulfovibrio-like characteristics (ie, they were gram-negative, motile rods that reduced sulfate, were inhibited by molybdate, and contained the pigment desulfoviridin). The remaining 6 isolates were gram-negative, nonmotile rods. Four of these released sulfide from cysteine, and 2 generated only limited amounts of sulfide from sulfate or cysteine. The 7 sulfate reducing isolates generated sulfide in rumen fluid broth medium at greater rates than those observed in fresh rumen fluid. Sulfate reduction Could be sustained in cultures for prolonged incubation times if the gas phase containing hydrogen sulfide was replaced at frequent intervals. Variations in the amount of sulfate reduced by the pure cultures were most pronounced at short incubation times. Sulfate reduction was not inhibited in mixed cultures of sulfate-reducing and nonsulfate-reducing bacteria.


Subject(s)
Cattle Diseases/chemically induced , Desulfovibrio/isolation & purification , Encephalomalacia/veterinary , Gram-Negative Anaerobic Bacteria/isolation & purification , Rumen/microbiology , Sulfates/adverse effects , Animals , Cattle , Desulfovibrio/classification , Desulfovibrio/physiology , Diet/veterinary , Encephalomalacia/chemically induced , Gram-Negative Anaerobic Bacteria/classification , Gram-Negative Anaerobic Bacteria/physiology , Male , Microbiological Techniques/veterinary , Phenotype , Sulfates/administration & dosage
13.
Am J Vet Res ; 56(10): 1390-5, 1995 Oct.
Article in English | MEDLINE | ID: mdl-8928960

ABSTRACT

Holstein steers were fed carbohydrate-rich, short-fiber basal diets with and without added sodium sulfate. Steers fed the high-sulfate diet developed the CNS disorder polioencephalomalacia (PEM). The onset of signs of PEM was associated with increased sulfide concentration in the rumen fluid. Over the course of the disease, anaerobic rumen bacteria were enumerated in roll tubes by use of the Hungate method Lo determine the effect of dietary sulfate on sulfate-reducing bacterial numbers. Media used included a general type for total counts and sulfate containing media with and without cysteine to assess sulfate-reducing bacteria. Changes in total and sulfate reducing bacterial numbers attributable to dietary sulfate content were not observed. The capacity to generate hydrogen sulfide from sulfate in fresh rumen fluid in vitro was substantially increased only after steers had been fed the high sulfate diet for 10 to 12 days, which coincided with the onset of signs of PEM. The low capacity for hydrogen sulfide production of rumen fluid taken at earlier times in the feeding period suggests that rumen microorganisms must adapt to higher dietary sulfate content before they are capable of generating potentially toxic concentrations of sulfide.


Subject(s)
Cattle Diseases/metabolism , Encephalomalacia/veterinary , Gram-Negative Bacteria/metabolism , Hydrogen Sulfide/metabolism , Rumen/microbiology , Sulfates/adverse effects , Animal Feed , Animals , Cattle , Cattle Diseases/chemically induced , Colony Count, Microbial/veterinary , Diet/veterinary , Encephalomalacia/chemically induced , Encephalomalacia/metabolism , Gram-Negative Bacteria/isolation & purification , Male , Sulfates/administration & dosage
14.
Am J Vet Res ; 55(12): 1762-70, 1994 Dec.
Article in English | MEDLINE | ID: mdl-7887524

ABSTRACT

4-Methylpyrazole (4-MP), an alcohol dehydrogenase inhibitor, was administered to dogs to treat ethylene glycol (EG) intoxication. Eleven dogs were given 10.6 g of EG/kg of body weight; 5 dogs were treated with 4-MP 5 hours after EG ingestion and 6 dogs were treated with 4-MP 8 hours after EG ingestion. 4-Methylpyrazole was administered IV as a 50-mg/ml [corrected] solution in 50% polyethylene glycol: initial dose, 20 mg/kg; at 12 hours after initial dose, 15 mg/kg; at 24 hours after initial dose, 10 mg/kg; and at 30 hours after initial dose, 5 mg/kg. Physical, biochemical, hematologic, blood gas, serum and urine EG concentrations, and urinalysis findings were evaluated at 0, 1, 3, 6, 9, 12, 24, 48, 72 hours, and at 1 week and 2 weeks after EG ingestion. Dogs of both groups developed clinicopathologic signs associated with EG intoxication, including CNS depression, hyperosmolality, high anion gap metabolic acidosis, polydipsia, polyuria, calcium oxalate monohydrate and dihydrate crystalluria, and isosthenuria. Fractional excretion of sodium was increased in all dogs between 1 and 9 hours after EG ingestion, but remained increased beyond 24 hours only in the 2 dogs treated at 8 hours after EG ingestion that developed acute renal failure. All dogs treated 5 hours after EG ingestion recovered without morphologic, biochemical, or clinical evidence of renal impairment. Of the 6 dogs treated 8 hours after EG ingestion, 2 developed acute renal failure. One of the dogs treated 8 hours after EG ingestion remained isosthenuric for 2 months, but did not manifest any other signs of renal impairment.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Dog Diseases/chemically induced , Dog Diseases/drug therapy , Ethylene Glycols/metabolism , Ethylene Glycols/poisoning , Pyrazoles/therapeutic use , Animals , Dog Diseases/blood , Dog Diseases/urine , Dogs , Ethylene Glycol , Female , Fomepizole , Male , Time Factors
15.
Am J Vet Res ; 55(12): 1771-82, 1994 Dec.
Article in English | MEDLINE | ID: mdl-7887525

ABSTRACT

The efficacy of 4-methylpyrazole (4-MP) and ethanol as treatment for ethylene glycol (EG) intoxication in cats was compared. Twenty-two cats were assigned at random to 6 experimental groups. Cats of 1 experimental group were given only 4-MP; those of another experimental group were given only EG. Cats of 3 experimental groups were intoxicated with EG and given 4-MP at 0 hour or 2 or 3 hours after EG ingestion, and those of 1 experimental group were given EG and treated with ethanol 3 hours after EG ingestion. Physical, biochemical, hematologic, blood gas, serum and urine EG concentrations, and urinalysis findings were evaluated at 0, 1, 3, 6, 9, 12, 24, 48, and 72 hours, 1 weeks, and 2 weeks after EG ingestion or 4-MP treatment in cats of the 4-MP only group. The half-life of EG and percentage of ingested EG excreted unchanged were determined for each group. 4-Methylpyrazole treatment at 0 hour was most effective at preventing metabolism of EG. 4-Methylpyrazole was not effective in preventing development of renal failure when given 2 or 3 hours after EG ingestion. Ethanol given 3 hours after EG ingestion was successful in preventing development of renal dysfunction in 2 of the 6 cats treated 3 hours after EG ingestion. Of the remaining 4 cats treated with ethanol, 2 developed transient renal dysfunction and 2 developed acute oliguric renal failure and were euthanatized. 4-Methylpyrazole given 2 or 3 hours after EG ingestion was less effective in preventing EG metabolism than was ethanol given 3 hours after EG ingestion. Therefore 4-MP, at the dose found to be effective in dogs, cannot be recommended as an alternative to ethanol for treatment of EG intoxication in cats.


Subject(s)
Cat Diseases/chemically induced , Cat Diseases/drug therapy , Ethanol/therapeutic use , Ethylene Glycols/metabolism , Ethylene Glycols/poisoning , Pyrazoles/therapeutic use , Animals , Cat Diseases/blood , Cat Diseases/urine , Cats , Ethylene Glycol , Female , Fomepizole , Male , Time Factors
16.
Am J Vet Res ; 53(11): 2125-35, 1992 Nov.
Article in English | MEDLINE | ID: mdl-1466511

ABSTRACT

Experimental evidence indicates that maintenance of urinary pH < or = 6.4 is the single most effective means of preventing feline struvite crystalluria or urolithiasis of noninfectious causes. This may be accomplished by dietary acidification, but must be moderated to avoid potential adverse effects of excessive acidification, including bone demineralization, negative calcium balance, potassium depletion, and renal disease. Effects of chronic dietary phosphoric acid supplementation on acid-base balance and on mineral and bone metabolism were investigated in adult, domestic cats. One group of 6 cats was fed a basal, naturally acidifying diet without added acidifiers, and another group of 6 cats was fed 1.7% dietary phosphoric acid. Changes observed during 12 months of study included development of noncompensated metabolic acidosis, increased urinary calcium excretion, and lower but positive calcium balance in cats of both groups. Urinary pH decreased in cats of both groups, but was significantly (P < 0.05) and consistently maintained < or = 6.4 in cats given dietary phosphoric acid. Urinary phosphorus excretion increased in cats of both groups, but was significantly (P < 0.05) greater in phosphoric acid-supplemented cats, leading to lower overall phosphorus balance as well. Potassium balance decreased in cats of both groups, but was only transiently negative in the phosphoric acid-supplemented cats midway through the study, and normalized at positive values thereafter. Plasma taurine concentration was not affected by dietary acidification, and remained well within the acceptable reference range for taurine metabolism. Double labeling of bone in vivo with fluorescent markers was followed by bone biopsy and histomorphometric measurement of several static and dynamic variables of bone formation. Overall indices of bone formation decreased in cats of both groups with age and confinement, but were not affected by dietary phosphoric acid supplementation. Dietary supplementation with phosphoric acid used as the principal inorganic P source to achieve moderate and stable degree of urinary acidification, did not appear over the course of 1 year, to have induced adverse effects on mineral, bone, or taurine balance in these adult domestic cats.


Subject(s)
Acid-Base Equilibrium/physiology , Bone and Bones/metabolism , Cats/metabolism , Minerals/metabolism , Phosphoric Acids/pharmacology , Acid-Base Equilibrium/drug effects , Animals , Bone and Bones/drug effects , Female , Food, Fortified , Hydrogen-Ion Concentration , Male
17.
J Comp Pathol ; 106(3): 267-78, 1992 Apr.
Article in English | MEDLINE | ID: mdl-1602060

ABSTRACT

Polioencephalomalacia (PEM) was induced in four of 10 lambs by the administration of a sulphide solution into the oesophagus at 20 min intervals for a period of 40 to 120 min. Signs of neurological dysfunction occurred in all 10 lambs during that time and included stupor, visual impairment and seizures. Gross autofluorescent and microscopic lesions in cerebrocortical grey matter were present as soon as 20 h after sulphide administration and were indistinguishable from lesions in naturally occurring disease. These findings, when considered together with an earlier study that revealed an association between high ruminal concentrations of sulphide and PEM, indicate that this disease can result from sulphide toxicosis, independent of the metabolic status of thiamine.


Subject(s)
Brain Diseases/veterinary , Cerebral Cortex , Sheep Diseases/chemically induced , Sulfides/adverse effects , Administration, Oral , Animals , Brain Diseases/chemically induced , Brain Diseases/pathology , Liver/pathology , Necrosis , Sheep , Sheep Diseases/pathology
18.
Am J Vet Res ; 53(3): 402-5, 1992 Mar.
Article in English | MEDLINE | ID: mdl-1534475

ABSTRACT

Although low plasma taurine concentrations have been associated with congestive cardiomyopathy in cats, the cause of taurine depletion in cats consuming adequate quantities of taurine is unknown. Taurine depletion and cardiovascular disease (cardiomyopathy and thromboembolism) developed unexpectedly in 3 of 6 healthy adult cats during a potassium-depletion study. Plasma taurine concentration decreased significantly (P less than 0.05) and rapidly over an 8-week period (from 98 to 36 nmol/ml) in 6 cats that consumed a potassium-deficient diet (0.20% potassium, dry matter basis) that was acidified with 0.8% ammonium chloride, despite containing dietary taurine concentrations (0.12% dry matter basis) in excess of amounts currently recommended. Taurine concentrations were significantly lower in cats fed the acidified diet than in 6 cats fed a potassium-deficient diet that was not acidified (36 nmol/ml vs 75 nmol/ml) after 8 weeks. In addition, plasma taurine concentrations did not decrease over a 6-month period in 8 cats that were fed a potassium-replete diet with acidifier. Plasma taurine concentrations were lowest in 3 cats that died of cardiovascular disease in the group receiving potassium-deficient, acidified diets. These data indicated an association between taurine and potassium balance in cats and suggested that development of taurine depletion and cardiovascular disease may be linked to concurrent potassium depletion.


Subject(s)
Cardiovascular Diseases/veterinary , Cat Diseases/etiology , Potassium Deficiency/veterinary , Taurine/deficiency , Ammonium Chloride/administration & dosage , Animal Feed , Animals , Cardiovascular Diseases/etiology , Cats , Female , Male , Potassium/administration & dosage , Potassium/blood , Potassium Deficiency/complications , Specific Pathogen-Free Organisms , Taurine/blood
19.
Am J Vet Res ; 53(2): 251-7, 1992 Feb.
Article in English | MEDLINE | ID: mdl-1575394

ABSTRACT

Zoometric measurements and bioelectrical impedance analysis were evaluated as methods of body composition determination in healthy cats. Zoometric and impedance measurements were taken on 22 anesthetized adult cats of various ages, genders, breeds, and body weights. The cats were then euthanatized. The bodies were processed through a tissue homogenizer and free-catch specimens were taken, freeze-dried, and analyzed for total body water, protein, fat, potassium, and ash content. Stepwise regression analysis was implemented to identify statistically significant relationships between the chemically determined dependent variables (total body water, protein, potassium, fat-free mass, fat mass, and percent body fat) and the zoometric measurements, with or without bioelectrical impedance analysis. Statistical analysis revealed high correlations between the dependent variables and the corresponding predicted values of those variables. Body weight alone was a poor predictor of body composition in these cats. On the basis of these findings, we suggest that zoometric and bioelectrical impedance measurements may serve as practical, noninvasive, simple, and accurate methods for estimating body composition in domestic cats.


Subject(s)
Body Composition , Cats/anatomy & histology , Animals , Electrodes/veterinary , Female , Male , Regression Analysis
20.
Am J Vet Res ; 52(7): 1164-9, 1991 Jul.
Article in English | MEDLINE | ID: mdl-1892274

ABSTRACT

Nine 115- to 180-kg, hay-adapted, Holstein steers were fed an experimental diet with added sodium sulfate that induces polioencephalomalacia (PEM). Five calves developed the disease. Thiamine concentrations in blood, CSF, brain, and liver were not indicative of thiamine deficiency. The odor of hydrogen sulfide in eructated rumen gas was associated with the onset of PEM. Sulfide concentrations in rumen fluid were measured 1 or 2 times a week by 2 techniques. Sulfide concentrations progressively increased in all 9 calves after the feeding of the PEM-inducing diet commenced. The highest concentrations coincided with the onset of clinical signs of PEM and were significantly higher in the calves that developed PEM than in those that did not. This suggests that PEM can result from sulfide toxicosis following excess production of sulfide in the rumen.


Subject(s)
Cattle Diseases/etiology , Diet , Encephalomalacia/veterinary , Rumen/chemistry , Sulfides/analysis , Animals , Brain Chemistry , Cattle , Encephalomalacia/etiology , Hydrogen-Ion Concentration , Liver/chemistry , Male , Thiamine/analysis , Thiamine/blood , Thiamine/cerebrospinal fluid
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