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1.
Ann Dermatol Venereol ; 147(5): 383-386, 2020 May.
Article in French | MEDLINE | ID: mdl-32107023

ABSTRACT

INTRODUCTION: Mucosal leishmaniasis is endemic in certain parts of Latin America and are usually absent in Morocco. Herein we report a case of Leishmaniainfantum in a Moroccan patient. PATIENTS AND METHODS: A 61-year-old male patient working as a tourist bus driver presented with a sublingual endobuccal tumor. He reported a history of treated cutaneous leishmaniasis of the lower lip in 2009 and had presented the sublingual oral tumor since December 2011. The histopathological findings as well as the species-specific PCR analysis confirmed the diagnosis of sublingual mucosal leishmaniasis due to L. infantum. HIV serology was negative. Our patient was then treated with intra-muscular meglumine antimoniate for 25 days, resulting in complete disappearance of the oral lesion. CONCLUSION: Our case thus has several peculiarities: the strictly mucosal character of the lesion, occurring in an immunocompetent subject, the unusual pseudotumoral form, and the causative agent, L. infantum, not known for its mucosal tropism in our country.


Subject(s)
Leishmania infantum , Leishmaniasis, Visceral , Tongue Diseases/parasitology , Humans , Leishmaniasis, Visceral/diagnosis , Leishmaniasis, Visceral/drug therapy , Male , Middle Aged , Tongue Diseases/diagnosis , Tongue Diseases/drug therapy
2.
Arch Pediatr ; 23(8): 832-5, 2016 Aug.
Article in French | MEDLINE | ID: mdl-27345558

ABSTRACT

BACKGROUND: Frontal fibrosing alopecia is a topographic form of lichen planopilaris, which most commonly affects postmenopausal women. We report on three original pediatric cases of this scarring alopecia, including one case of female twins. OBSERVATIONS: The first observation concerns twin sisters, 14 years of age, with frontotemporal symmetric and progressive alopecia, beginning at the age of 5 years, with follicular facial noninflammatory micropapules. Histological examination showed a depletion of hair follicles with dermal fibrosis and perivascular infiltrate. The treatment decided was monthly intralesional infiltrations of betamethasone. The second case is a 7-year-old girl, with a frontotemporal alopecia band and partial alopecia of the eyebrows. Histological examination was consistent with lichen planopilaris. The outcome was favorable after six monthly oral boluses of steroids. COMMENT AND CONCLUSION: The initial description of frontal fibrosing alopecia was made in postmenopausal women. Some cases have been reported subsequently in premenopausal women as well as in men, with recent identification of familial cases. To our knowledge, these are the first pediatric cases of this form of alopecia, which is not only postmenopausal.


Subject(s)
Alopecia/etiology , Lichen Planus/diagnosis , Adolescent , Alopecia/drug therapy , Child , Female , Glucocorticoids/therapeutic use , Humans , Lichen Planus/drug therapy
3.
J Mal Vasc ; 40(6): 402-5, 2015 Dec.
Article in French | MEDLINE | ID: mdl-26548536

ABSTRACT

UNLABELLED: Vitamin K antagonists are widely used in thromboembolic diseases. Hemorrhagic complications related to drug overdose represent their main side effect. We report a rare side effect, a severe and unexpected type of skin vasculitis - necrotic leg ulcer - induced by vitamin K antagonist. CASE REPORT: A 63-year-old female with a history of diabetes developed hyperalgesic necrotic ulcerations on the lower limbs one month after starting an acenocoumarol-based treatment for ischemic heart disease. Histological examination revealed lymphocytic vasculitis with fibrinoid necrosis. Etiological explorations searching for vasculitis were negative. In the absence of a precise etiology, drug-induced ulcer was suspected. Low molecular weight heparin was prescribed to replace acenocoumarol. The lesions slowly resolved with topical treatment. DISCUSSION: The chronological criteria and the negativity of etiological explorations allowed the diagnosis of vitamin K antagonist-induced necrotic skin ulcer. Clinicians should be aware of this rare complication induced by oral anticoagulants because of its practical therapeutic implications. This is the first case of necrotic leg ulcer induced by acenocoumarol corresponding histologically to necrotising lymphocytic vasculitis.


Subject(s)
Acenocoumarol/adverse effects , Anticoagulants/adverse effects , Leg Ulcer/etiology , Vasculitis/chemically induced , Vitamin K/antagonists & inhibitors , Acenocoumarol/therapeutic use , Anticoagulants/therapeutic use , Diabetes Mellitus, Type 2/complications , Drug Substitution , Female , Heparin, Low-Molecular-Weight , Humans , Hyperalgesia/etiology , Leg Ulcer/pathology , Middle Aged , Necrosis , Vasculitis/complications , Vasculitis/immunology
5.
Chir Main ; 32(3): 183-5, 2013 Jun.
Article in French | MEDLINE | ID: mdl-23538099

ABSTRACT

Schwannoma is a tumor that develops from nerve sheath. The authors report an original observation of a giant schwannoma developed in the arm depending on the musculocutaneous nerve. The diagnosis was based on MRI appearance. Confirmation of the diagnosis was made by histological examination. Surgical treatment was resection, taking care preserving adjacent nerve fibers. This allowed recovery without any sequela. Recurrence rate and potential for malignant transformation of this type of tumor is low.


Subject(s)
Musculocutaneous Nerve/surgery , Nerve Sheath Neoplasms/pathology , Nerve Sheath Neoplasms/surgery , Neurilemmoma/pathology , Neurilemmoma/surgery , Adult , Arm/surgery , Biopsy , Humans , Magnetic Resonance Imaging , Nerve Sheath Neoplasms/diagnosis , Neurilemmoma/diagnosis , Neurosurgical Procedures , Prognosis , Treatment Outcome
7.
Rev Chir Orthop Reparatrice Appar Mot ; 89(4): 357-60, 2003 Jun.
Article in French | MEDLINE | ID: mdl-12844040

ABSTRACT

Ossifying parosteal lipoma is an uncommon form of parosteal lipoma. We report an exceptional rib localization where hyperosteosis probably arose from contact between the lipoma and the periosteum. Foci of metaplastic cartilage undergoing chondral ossification within the adipose tissue suggest a possible mixed origin.


Subject(s)
Bone Neoplasms , Lipoma , Ribs , Adult , Bone Neoplasms/diagnosis , Bone Neoplasms/surgery , Humans , Lipoma/diagnosis , Lipoma/surgery , Male
8.
Article in French | MEDLINE | ID: mdl-11976575

ABSTRACT

We report the case of 37-year-old women with primary Squamous cell carcinoma of the right breast. The tumor was nodular, 13cm, with important hemorrhagic and necrotic reshaping. There was no central or peripheral cystic altering. Histologically, the tumoral cells were polyedric, dyskeratosic with intercellular junction. This rare breast entity has controversy histogenesis. The development was characterized by metastases frequency. The treatment remains no codified.


Subject(s)
Breast Neoplasms/pathology , Carcinoma, Squamous Cell/pathology , Adult , Breast Neoplasms/diagnosis , Breast Neoplasms/therapy , Carcinoma, Squamous Cell/diagnosis , Carcinoma, Squamous Cell/therapy , Female , Hemorrhage , Humans , Necrosis
9.
Eur J Biochem ; 254(1): 90-5, 1998 May 15.
Article in English | MEDLINE | ID: mdl-9652399

ABSTRACT

This study describes the cloning of two cDNAs encoding three serine-protease-inhibiting peptides, SGPI I, II and III, which were recently identified from ovarian extracts of the desert locust, Schistocerca gregaria. The first cDNA codes for the precursor polypeptides of SGPI I and SGPI II; the second encodes only a single inhibitor, SGPI III. Northern-blot analysis revealed an approximate length of 0.8 kb for SGPI-I/II mRNA and 0.6 kb for SGPI-III mRNA. The transcripts are present in several locust tissues, but they could not be detected in the midgut. The gene for SGPI-I/II is abundantly transcribed during all larval and adult stages, whereas SGPI-III mRNA is mainly present in adults. Northern-blot hybridization also revealed important changes in the SGPI-mRNA content during the molting cycle and during the adult reproductive cycle. Moreover, a differential hormonal control was observed in adult females which had been treated with precocene, juvenile hormone or ecdysone.


Subject(s)
Grasshoppers/physiology , Insect Proteins/chemistry , Peptides/chemistry , Serine Proteinase Inhibitors/chemistry , Amino Acid Sequence , Animals , Base Sequence , Benzopyrans/pharmacology , Cloning, Molecular , Ecdysterone/pharmacology , Female , Gene Expression Regulation, Developmental/genetics , Molecular Sequence Data , Ovary/chemistry , Protein Precursors/chemistry , RNA, Messenger/metabolism , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Sesquiterpenes/pharmacology , Transcription, Genetic/genetics
10.
Biochem Biophys Res Commun ; 243(2): 390-4, 1998 Feb 13.
Article in English | MEDLINE | ID: mdl-9480819

ABSTRACT

A novel, highly hydrophobic, glycine- and proline-rich peptide was characterized in the ovary of the desert locust, Schistocerca gregaria. The peptide was detected as one of the major peaks in a chromatographic separation of an acidic methanolic extract of 50 mature ovaries. Electrospray mass spectrometry yielded a molecular mass of 6305 Da. The partial amino acid sequence as determined by Edman degradation based automated microsequencing is as follows: Ala-Tyr-Pro-Ala-His-Gln-Gly-Tyr- Pro-Ala-His-Val-Gly-Tyr-Ala-Arg-Val-Gly-Tyr-Gly- Gly-Tyr-Pro-Ser-Tyr-Gly-Tyr-Pro-Ala. Four amino acids (Gly, Pro, Ala, and Tyr) account for more than 80% of the composition of this sequence. Gly-Tyr-Pro is the most important repetitive motif. Ala-Tyr-Pro, Gly-Tyr-Gly and Gly-Tyr-Ala occur as variations of this motif. The novel glycine- and proline-rich insect peptide displays structural characteristics similar to those of a new class of glycine- and proline-rich proteins (GPRP) that have recently been identified in Arabidopsis thaliana (thale cress) and Daucus carota (carrot). The GPRP of A. thaliana contains the same repetitive motifs (except for Ala-Tyr-Pro), the Gly-Tyr-Pro motif also being the most abundant.


Subject(s)
Arabidopsis Proteins , Grasshoppers/chemistry , Peptides/chemistry , Plant Proteins/chemistry , Amino Acid Sequence , Animals , Arabidopsis/chemistry , Daucus carota/chemistry , Female , Molecular Sequence Data , Ovary/chemistry , Peptides/isolation & purification , Sequence Analysis , Sequence Homology, Amino Acid
11.
FEBS Lett ; 422(1): 74-8, 1998 Jan 23.
Article in English | MEDLINE | ID: mdl-9475173

ABSTRACT

The ovary of the desert locust, Schistocerca gregaria, contains multiple inhibitors of serine proteases. Five serine protease inhibitors, designated SGPI-1-5 (Schistocerca gregaria protease inhibitors) were purified from methanolic extracts of mature ovaries and analyzed by mass spectrometry and amino acid sequencing. The revealed primary structures display amino acid similarities and are related to the serine protease inhibitors identified in the hemolymph of Locusta migratoria. All inhibitors show an in vitro inhibiting activity towards alpha-chymotrypsin. In addition, SGPI-1 displays in vitro inhibiting activity towards trypsin, and SGPI-2 is a potent pancreatic elastase inhibitor. Differences in inhibitory specificities towards the locust endogenous serine proteases can be readily attributed to the amino acid sequence within the active region and also to amino acid residues beyond the P1-P'1 bond. A difference in one or two amino acid residues around the reactive sites results in considerable alteration of the inhibitory specificity. The temporal and spatial distribution of SGPI-1-5 was studied by RP-HPLC analysis. All inhibitors occur in hemolymph, ovaries, testes and fat body of adults but are absent in the gut. They are also present in larval hemolymph and fat body. An antibody raised against SGPI-2 shows positive immunostaining in the ovarian follicle cells.


Subject(s)
Ovary/chemistry , Serine Proteinase Inhibitors/chemistry , Serine Proteinase Inhibitors/isolation & purification , Amino Acid Sequence , Animals , Binding Sites , Chymotrypsin/antagonists & inhibitors , Female , Grasshoppers , Immunohistochemistry , Isoenzymes/isolation & purification , Isoenzymes/pharmacology , Mass Spectrometry , Molecular Sequence Data , Molecular Weight , Pancreatic Elastase/antagonists & inhibitors , Peptides/isolation & purification , Peptides/pharmacology , Sequence Analysis , Trypsin Inhibitors/pharmacology
12.
Biochem Biophys Res Commun ; 238(2): 357-60, 1997 Sep 18.
Article in English | MEDLINE | ID: mdl-9299512

ABSTRACT

A protease-inhibitor was isolated from mature ovaries of Schistocerca gregaria by a combination of trypsin-affinity chromatography and reverse-phase high performance liquid chromatography. It was characterized by aminoterminal amino acid sequencing using Edman degradation based automated microsequencing and by MALDI-TOF mass spectrometry. The N-terminal sequence (Y)XAEXDELA(A)EEY(Y)Q(Q)X(I)(L)M (X being a Cys, an irregular or modified amino acid) revealed no similarities with any other protease inhibitors isolated from invertebrate or vertebrate source. The 14 kDa inhibitor was found to be heat-stable. It shows potent inhibitory activity toward bovine trypsin and chymotrypsin, but not toward pancreatic elastase. It is likely that the characterized inhibitor will serve as an important tool for understanding its role in insect development.


Subject(s)
Grasshoppers/metabolism , Ovary/metabolism , Serine Endopeptidases/isolation & purification , Animals , Cattle , Female , Hot Temperature , Mass Spectrometry , Serine Endopeptidases/metabolism
13.
Am Rev Respir Dis ; 135(4): 860-3, 1987 Apr.
Article in English | MEDLINE | ID: mdl-3105373

ABSTRACT

Pseudomonas aeruginosa may cause severe lung infections in humans. This bacteria secretes an elastase that might degrade lung elastin. We have studied the solubilization of human lung elastin by P. aeruginosa elastase in an attempt to delineate the pathogenic role of this proteinase in P. aeruginosa lung infections. We also used bovine ligamentum nuchae elastin and human leukocyte elastase for comparative purposes. With an elastin concentration of 5 mg X ml-1 and at physiologic ionic strength, P. aeruginosa elastase is about 50 times more active on human lung elastin than on bovine elastin. In contrast, human leukocyte elastase has similar specific activities on the 2 substrates. In addition, the bacterial enzyme is about 10 times more active on human elastin than the neutrophil elastase but the latter is about 5 times more active on bovine elastin than the former. In order to better quantitate these enzyme-substrate interactions, we have measured initial rates of elastolysis, derived from product versus time curves, as a function of elastin concentration. The substrate-velocity curves, analyzed using an equation similar to the classic Michaelis-Menten one, yielded 2 empirical kinetic parameters: [S50]-1, the apparent elastase-elastin affinity and Vm, the apparent catalytic efficiency of elastase. This analysis shows that human leukocyte elastase exhibits similar [S50]-1 and Vm values for the 2 elastins. The low activity of P. aeruginosa elastase on bovine elastin is due to the combined effects of low S50(-1) and Vm values, which could not be measured separately.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Bacterial Proteins , Elastin/metabolism , Endopeptidases/metabolism , Lung/metabolism , Metalloendopeptidases , Animals , Cattle , Humans , In Vitro Techniques , Kinetics , Pseudomonas aeruginosa/pathogenicity , Solubility , Time Factors
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