ABSTRACT
A simple, accurate, and sensitive HPLC analysis of monoethylglycinexylidide (MEGX) and lidocaine in porcine microsome samples is described. Lidocaine and MEGX were measured by direct injection after the addition of the internal standard. Chromatography was performed on a muBondapak C(18) column using an isocratic mobile phase of 0.03 M potassium dihydrogen phosphate:acetonitrile (87:13), pH 5.9. UV absorbance was measured at 205 nm. The procedure produced linear curves for the concentration range 50-1000 ng/mL with a limit of detection of 10 ng/mL. Recoveries for both compounds were greater than 90%. This assay produced accurate and repeatable results.
Subject(s)
Lidocaine/analogs & derivatives , Lidocaine/analysis , Microsomes/chemistry , Animals , Chromatography, High Pressure Liquid , Indicators and Reagents , Lidocaine/metabolism , Reference Standards , Solutions , Spectrophotometry, Ultraviolet , SwineABSTRACT
A simple, accurate and sensitive HPLC method for the in vitro determination of 6-hydroxychlorzoxazone and chlorzoxazone in porcine microsome samples is described. Chromatography was performed on a YMC-Pack ODS-AQ column using a mobile phase of 0.05% phosphoric acid pH 3-methanol (60:40, v/v). UV detection was carried out at 287 nm. The detector response was linear over the concentration range 25-2000 ng/ml. This assay produced quick, accurate, and repeatable results.
