Bisphenol A release from CAD/CAM splint materials.

This study aimed to investigate the bisphenol A (BPA) release from four CAD/CAM splint materials: three polycarbonate-based (DD BioSplint C, Splint Plus Biostar, Temp Premium Flexible) and one polymethylmethacrylate-based (Temp Basic) material. From each material, ten cylindrical samples (n = 40) were immersed in high-performance liquid chromatography (HPLC) grade water following ISO 10993-12 and incubated for 24 h in an incubation shaker at 37°C and 112 rpm. Following BPA derivatization, analysis was performed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). After 24 h of incubation, all investigated materials released significant amounts of BPA compared to water blanks. The material-dependent elution increased in the following order: DD BioSplint C < Splint Plus Biostar < Temp Basic < Temp Premium Flexible. Subtracting extraneous BPA, the concentrations ranged between 2.27 ng/mL and 12.65 ng/mL. After extrapolating the concentrations in relation to the average surface area of occlusal splints, the amount of BPA per mL exceeded the Tolerable Daily Intake (TDI) set by the European Union for a person weighing 70 kg by 1.32-6.16 times. Contrary to the release from previously investigated materials, BPA elution from CAD/CAM splint materials was highly elevated. Considering the increasing adaptation of CAD/CAM techniques, elution from them may represent a relevant BPA source in daily dental practice.

How to register static occlusion - Correlation of contemporary techniques.

Statement of problem: A working knowledge of the analytical capacities of contemporary registration methods is essential for prosthetic treatment; however, there is a paucity of studies which coherently investigate the capabilities and limitations of the various diagnostic procedures utilized for prosthetic occlusion. Purpose: The present prospective clinical study aimed to evaluate the similarities and differences among contemporary registration methods through comparative analysis. Material and methods: The habitual static occlusion of 19 healthy individuals (14 women; mean age ± standard deviation, 30.8 ± 4.8 years) was analyzed 3 times a day, using shimstock foil, occlusal foil, wax registration, silicone registration, and computerized registration. The procedures were repeated after 14 days. Statistical analyses included all registrations referencing the first measurement point to assess the mean values of antagonistic contacts and the differences between these measurements. Pearson's and Kendall's correlation analyses were performed as part of the coherent mixed logistic regression model, and marginal probabilities were calculated using the registration technique and repeated measurements. Results: Strong correlations were found among the various registration techniques. The largest effect sizes were observed among the wax, silicone, occlusion foil, and computerized registrations (r = 0.95, P < 0.001 to r = 0.62, P < 0.001), while the lowest effect sizes were found for shimstock correlations (τ = 0.41, P < 0.001 to τ = 0.27, P < 0.001). Occlusal changes per maxillary arch were observed referencing the first measurement time with wax registration (P < 0.001; 7.4%), shimstock foil (P < 0.001; 13.8%), computerized registration (P < 0.001; 20.3%), silicone registration (P = 0.009; 66.3%), and occlusion foil (P < 0.001; 98.8%). Occlusal changes per maxillary tooth were observed from the first incisor (P < 0.001; 5.7%) to the third molar (P < 0.001; 18.1%). Conclusions: The results of the present study revealed that there are strong overall correlations among the various contemporary registration techniques. The different affinities of the techniques used to register occlusal changes, however, showed differences in the measurement techniques, which should be neither over- nor underestimated. The differential tendencies of teeth to change should be considered, even if a hypervariable system is assumed.

In Vivo Biofilm Formation on Novel PEEK, Titanium, and Zirconia Implant Abutment Materials.

The formation of biofilms on the surface of dental implants and abutment materials may lead to peri-implantitis and subsequent implant failure. Recently, innovative materials such as polyether-ether-ketone (PEEK) and its modifications have been used as abutment materials. However, there is limited knowledge on microbial adhesion to PEEK materials. The aim of this in vivo study was to investigate biofilm formation on the surface of conventional (titanium and zirconia) and PEEK implant abutment materials. Split specimens of titanium, zirconia, PEEK, and modified PEEK (PEEK-BioHPP) were manufactured, mounted in individual removable acrylic upper jaw splints, and worn by 20 healthy volunteers for 24 h. The surface roughness was determined using widefield confocal microscopy. Biofilm accumulation was investigated by fluorescence microscopy and quantified by imaging software. The surface roughness of the investigated materials was <0.2 µm and showed no significant differences between the materials. Zirconia showed the lowest biofilm formation, followed by titanium, PEEK, and PEEK-BioHPP. Differences were significant (p < 0.001) between the investigated materials, except for the polyether-ether-ketones. Generally, biofilm formation was significantly higher (p < 0.05) in the posterior region of the oral cavity than in the anterior region. The results of the present study show a material-dependent susceptibility to biofilm formation. The risk of developing peri-implantitis may be reduced by a specific choice of abutment material.

A Pilot Study on Monomer and Bisphenol A (BPA) Release from UDMA-Based and Conventional Indirect Veneering Composites.

This study aimed to investigate the release of common monomers from conventional (Dialog Vario, Enamel Plus HFO) and UDMA-based indirect veneering composites (VITA VM LC, GC Gradia). Ten cylindrical samples of each material were prepared (n = 40), immersed in HPLC grade water, and incubated for 24 h in an incubation shaker at 37 °C and 112 rpm. Extraction was performed following ISO 10993-12 and monomers were detected and quantified by HPLC-MS/MS. In all the samples, urethane dimethacrylate (UDMA) and bisphenol A (BPA) were quantifiable. Compared to water blanks, BPA levels were only elevated in the eluates from conventional composites. In all other samples, concentrations were in the range of extraneous BPA and were therefore clinically irrelevant. Low concentrations of Bisphenol A-glycidyl methacrylate (BisGMA) were found in one BPA-free composite and in both conventional materials. Statistical analyses showed that BPA-free materials released significantly less BisGMA and no BPA, while UDMA elution was comparable to elution from conventional materials. All measured concentrations were below reported effective cytotoxic concentrations. Considering these results, the substitution of BPA-derivatives with UDMA might be beneficial since BPA-associated adverse effects are ruled out. Further studies should be enrolled to test the biocompatibility of UDMA on cells of the oral environment.

Changes in Maximum Mandibular Mobility Due to Splint Therapy in Patients with Temporomandibular Disorders.

Splint therapy is widely used in the treatment of myofascial pain, but valid studies on the efficacy of this therapy are rare. The purpose of the present study was to investigate which qualifiable and quantifiable effects of splint therapy are detectable. For this purpose, 29 patients (21 women, mean age 44.6 ± 16 years) diagnosed with myofascial pain (RDC/TMD) were investigated in this prospective clinical trial (10/6/14An). Patients were treated with Michigan splints applied overnight for three months. Before (T1) and after three months of treatment (T2), patients were registered with an electronic ultrasound device with qualitative and quantitative evaluation of the registrations and a qualitative assessment of pain symptoms using a verbal analog scale. Significant differences were found between maximum mouth opening (MMP) (p < 0.001) and right condylar movement (CM) at MMP (p = 0.045). Qualitative assessment revealed that 24 of 29 patients experienced an improvement in pain symptoms, 17 of whom experienced complete remission. The results of the qualitative and quantitative analysis provide indications of the effectiveness of the splint therapy. In addition to quantitative measurements, the ultrasound facebow technique was also able to provide qualitative information.

Monomer Release from Dental Resins: The Current Status on Study Setup, Detection and Quantification for In Vitro Testing.

Improvements in mechanical properties and a shift of focus towards esthetic dentistry led to the application of dental resins in various areas of dentistry. However, dental resins are not inert in the oral environment and may release monomers and other substances such as Bisphenol-A (BPA) due to incomplete polymerization and intraoral degradation. Current research shows that various monomers present cytotoxic, genotoxic, proinflammatory, and even mutagenic effects. Of these eluting substances, the elution of BPA in the oral environment is of particular interest due to its role as an endocrine disruptor. For this reason, the release of residual monomers and especially BPA from dental resins has been a cause for public concern. The assessment of patient exposure and potential health risks of dental monomers require a reliable experimental and analytical setup. However, the heterogeneous study design applied in current research hinders biocompatibility testing by impeding comparative analysis of different studies and transfer to the clinical situation. Therefore, this review aims to provide information on each step of a robust experimental and analytical in vitro setup that allows the collection of clinically relevant data and future meta-analytical evaluations.

A comparative in vitro study on monomer release from bisphenol A-free and conventional temporary crown and bridge materials.

This study aimed to investigate the release of common monomers from two conventional and two bisphenol A (BPA)-free temporary crown and bridge materials. Cylindrical samples of all materials were prepared (N = 90; five samples for each material and cycle of analysis). All samples were immersed in high-performance liquid chromatography (HPLC)-grade water and incubated for 1 h, 12 h, 24 h, and 7 days in an incubation shaker at 37°C and 112 rpm. Extraction was performed in accordance with ISO 10993-12. Eluted monomers were detected and quantified by HPLC coupled with ultraviolet-visible spectroscopy and mass spectrometry (HPLC-UV/Vis-MS). Analysis of BPA was performed by HPLC coupled with ultraviolet-visible spectroscopy (HPLC-UV/Vis) and positive results were verified by HPLC-tandem mass spectrometry (HPLC-MS/MS). Neither bisphenol A-glycidyl methacrylate (Bis-GMA) nor BPA was quantifiable in any of the crown and bridge samples investigated in the present study. However, all samples contained triethylene glycol dimethacrylate (TEGDMA) and/or urethane dimethacrylate (UDMA) after 24 h of incubation. Statistical analysis showed that significantly more UDMA was released from the BPA-free materials than from the conventional materials. All concentrations of UDMA measured were below the effective cytotoxic concentrations previously reported. However, for a few materials, especially BPA-free temporary crown and bridge materials, the levels of UDMA were above previously reported potentially harmful concentrations for local cells. As BPA-free materials were introduced as being more biocompatible than materials containing BPA, substitution of Bis-GMA with UDMA should be further investigated.

Intraoral sensor-based monitoring of stabilization splint therapy in patients with myofascial pain.

AIM: The present study aimed to evaluate intraoral microsensors for the objective measurement of patient compliance during splint therapy and to comparatively analyze the duration of mandibular and maxillary splint application in patients with myofascial pain. MATERIALS AND METHODS: Thirty-two patients with sole myofascial pain without limited opening (Research Diagnostic Criteria for Temporomandibular Disorders [RDC/TMD] Ia) were divided into two groups. To ensure patient comparability, all pressure-sensitive sites from the initial palpation were summarized as pain scores. The subjects in group 1 were treated with maxillary stabilization splints, and those in group 2 with mandibular stabilization splints. All splints were equipped with a microsensor without interfering static or dynamic occlusion. Wear pattern was recorded at three intervals of 30 days each. Following the observation period, the data were retrieved and statistically evaluated using multi-factor analysis of variance (ANOVA) and the Bland-Altman analysis. RESULTS: During the observation period, maxillary splints were applied in 44.4% and mandibular splints in 44.2% of the days. Regarding patient compliance, there was no significant difference between the maxillary and mandibular splints (P = 0.359). Patients with an increased pain score (P < 0.0001) and female patients (P = 0.013) wore their splints significantly more often. The wear time decreased over the observation period, whereas only the initial and terminal interval differed significantly across both the mandibular and maxillary splint groups (P < 0.0001). CONCLUSION: The microsensor used in the present study was an effective and reliable tool for monitoring patient compliance in patients with temporomandibular disorders (TMD). This method also allows for the reliable recording of intraoral splint application prior to the required bite elevations. The two types of splints analyzed in the present study had no significant influence on compliance.

The variability of the curve of Spee : An analysis of multiple setups of the same Angle Class I patient case.

PURPOSE: The aim of this study was to examine possible parameters correlating to the shape of the curve of Spee (COS) by analyzing multiple setups of the same Angle class I patient case. METHODS: In all, 65 setups of the same Angle class I patient case were constructed. We measured the depth of the COS, length and width of the dental arch, steps, rotations, spaces and symmetry of the constructed setups. With a correlation analysis, possible relationships between the depth of the COS and measured parameters were evaluated. RESULTS: The COS in the constructed setups was more exaggerated than in the reference case but none of the measured parameters correlated to the depth of the COS. The largest deviation was found on the mesiobuccal cusp tip of the first molar (-0.5 ± 0.4 mm left side; -0.8 ± 0.5 mm right side). This study found a negative correlation between the length and width of the lower dental arch (r = -0.61). While maintaining Angle class I, a large spread width of the depth of the COS, the dental arch width and length was shown. CONCLUSION: The different manifestations of the COS in this study show the freedom of arrangement of the individual tooth morphology without exhibiting a correlation to parameters of the dental arch.