ABSTRACT
The development of optical sensors for metal ions has gained significant attention due to their broad applications in biology, the environment, and medicine. Colorimetric and fluorometric detection methods are particularly valued for their simplicity, cost-effectiveness, high detection limits, and analytical power. Among various chemical probes, the hydrazone functional group stands out for its extensive study and utility, owing to its ease of synthesis and adaptability. This review provides a comprehensive overview of N-acylhydrazone-based probes, serving as highly effective colorimetric and fluorometric chemosensors for a diverse range of metal ions. Probes are categorized into single-ion, dual-ion, and multi-ion chemosensors, each further classified based on the detected metal(s). Additionally, the review discusses detection modes, detection limits, association constants, and spectroscopic measurements.
ABSTRACT
In this study, a novel Benzil Bis-Hydrazone (BBH) sensor with two C = N-N = C moieties was designed and synthesized based on the condensation reaction between benzil-dihydrazone (b) and cinnamaldehyde. The BBH probe in dimethylsulfoxide showed extremely weak fluorescence. However, the same solution exhibited an intensive fluorescence enhancement (152-fold) with the introduction of Zn(II) ions. In contrast, no or negligible fluorescence changes were observed when other ions were added. The fluorogenic behavior of BBH towards the examined cations indicated an excellent selectivity of the BBH sensor for Zn(II) cations without any interference from other cations like Fe(II), Mg(II), Cu(II), Co(II), Mn(II), Cr(III), Hg(II), Sn(II), Al(I), La(III), Ca(II), Ba(II), Na(I), K(I), and especially Cd(II). Moreover, the UV-vis spectrophotometric titrations revealed that a 1:1 stoichiometric complex BBH-Zn(II) was formed during the Zn(II) sensing and the binding constant value for this complex was calculated to be equal to 106.8. Further, in order to show the affinity of the BBH sensor for Zn(II) cations, it was deemed necessary to determine the limit of detection (LOD) which was found to equal to 2.5 10-4 M.
