Effect of preoperative sleep disorders on delirium in proximal femoral surgery patients aged 60 or older.

OBJECTIVE: To examine the effect of preoperative sleep disorders on delirium in patients older than 60 years of age who underwent surgery for proximal femoral fracture. METHODS: This is a prospective observational study. We prospectively selected 143 patients with proximal femoral fracture who underwent surgery between April 2021 and April 2022. The primary outcome was postoperative delirium (PD). Multiple logistic regression analyses were performed and a receiver operating characteristic (ROC) curve was generated. The preoperative sleep quality of all eligible participants was assessed through the Pittsburgh Sleep Quality Index (PSQI). The Confusion Assessment Method (CAM) was used to assess PD from the first to the seventh day postoperatively. Patients were divided into two groups according to the PD diagnosis: (1) the no PD (NPD) group and (2) the PD (PD) group. RESULTS: Of 143 eligible patients, 43 (30.1%) were diagnosed with PD. Multiple logistic regression analysis demonstrated that postoperative ICU admissions (OR = 2.801, p = 0.049) and preoperative sleep disorders (OR = 1.477 p < 0.001) were independently associated with PD. A receiver operating characteristic (ROC) curve demonstrated that the preoperative PSQI score was predictive of PD (AUC 0.808, 95% CI 0.724 ~ 0.892, p < 0.001). CONCLUSION: Preoperative sleeping disorders may be an independent risk factor leading to PD and an independent predictive factor for the development of delirium in proximal femoral surgery patients aged 60 or older.

Prevalence of Neisseria meningitidis serogroups in invasive meningococcal disease in China, 2010 - 2020: a systematic review and meta-analysis.

Invasive meningococcal disease (IMD) caused by Neisseria meningitidis (Nm) continues to be a global public health concern. Understanding the prevalence of Nm serogroups in IMD is critical for developing strategies for meningococcal vaccination. We used the keywords "cerebrospinal meningitis", "meningococcal", "Neisseria meningitidis'', "meningococcal meningitis", "serogroup'' and "China'' to search five databases, including PubMed, CNKI, CBM (Chinese BioMedical Literature Database), WanFang and VIP from 2010 to 2020. The age distributions, proportions of Nm serogroups and serogroup changes in IMD were analyzed. A total of 14 studies were included according to PRISMA guidelines. In China, from 2010 to 2020, the highest proportion of Nm in IMD was NmC, with 49.7% (95% CI: 35.8%-63.5%), followed by NmB with 30.2% (95%CI:17.3%-43.0%) and NmW with 23.8% (95%CI: 7.0-40.7%). Before 2014, NmC was the major circulating serogroup, with 59.6% (95% CI: 43.8%-75.4%), followed by NmW with 24.4% (95% CI: 5.9%-42.9%). After 2015, IMD cases caused by NmB were increasing, the proportion of NmB reached to 52.4% (95% CI: 31.8%-73.1%). The age groups of children from 0 to 5 years and from 6 to 10 years represented, respectively, 29.6% (95% CI: 16.8%-42.4%) and 28.9% (95% CI: 12.1%-45.8%) of all IMD cases were reported. In China, NmB, NmC and NmW were the major serogroups causing IMD between 2010 and 2020. Since 2015, the proportion of NmB increased rapidly. The current serogroup distribution in China highlights the need of replacing the meningococcal polysaccharide vaccines that are being used in the National Immunization Program with more appropriate vaccines.

Multilayer structured CNF/rGO aerogels and rGO film composites for efficient electromagnetic interference shielding.

The development of efficient electromagnetic interference (EMI) shielding materials with high electromagnetic waves (EMWs) absorption capacity is of great significance to alleviate secondary EMWs pollution. Herein, multilayered composites were prepared by stacking cellulose nanofibril (CNF)/reduced graphene oxide (rGO) aerogels and rGO film together. The porous aerogels and the dense film serve as the EMWs absorbing layer and reflecting layer, respectively. When the EMWs enter the multilayer composites, they go through the process of absorption-reflection-reabsorption, resulting in a high EMI shielding effectiveness (SE) of ~32 dB. Furthermore, both experimental and theoretical analyses were adopted to explore the effect of arrangement order of CNF/rGO aerogels on EMI shielding performance. The results indicate that composites with progressively higher graphene content exhibit a higher EMWs absorption capacity at the same total EMI SE. This work offers a feasible design for improving EMWs absorption without affecting the overall EMI shielding performance of the material.

PAK5 promotes RNA helicase DDX5 sumoylation and miRNA-10b processing in a kinase-dependent manner in breast cancer.

P21-activated kinase 5 (PAK5) plays an important role in tumors. However, the functional role of PAK5 in mammary tumorigenesis in vivo remains unclear. Here, we show that PAK5 deficiency represses MMTV-PyVT-driven breast tumorigenesis. DEAD-box RNA helicase 5 (DDX5) is a substrate of PAK5, which is phosphorylated on threonine 69. PAK5-mediated DDX5 phosphorylation promotes breast cancer cell proliferation and metastasis. The increased expression levels of PAK5 and phospho-DDX5 threonine 69 are associated with metastasis and poor clinical outcomes of patients. PAK5 facilitates the phosphorylation-dependent sumoylation of DDX5 to stabilize DDX5. Both the phosphorylation and sumoylation of DDX5 enhance the formation of a DDX5/Drosha/DGCR8 complex, thus promoting microRNA-10b processing. Finally, we verify decreased expression of DDX5 phosphorylation and sumoylation and mature miR-10b in PAK5-/-/MMTV-PyVT transgenic mice. Our findings provide insights into the function of PAK5 in microRNA (miRNA) biogenesis, which might be a potential therapeutic target for breast cancer.

PAK5-mediated AIF phosphorylation inhibits its nuclear translocation and promotes breast cancer tumorigenesis.

Although p21 activated kinase 5 (PAK5) is related to the progression of multiple cancers, its biological function in breast cancer remains unclear. Apoptosis-inducing factor (AIF) is a vital apoptosis factor in mitochondria, which can be released from mitochondria and enter the nucleus, causing caspase-independent apoptosis. In this study, we reveal that PAK5 inhibits apoptosis by preventing the nuclear translocation of AIF. PAK5 inhibits the release of AIF from mitochondria in breast cancer cells by decreasing the mitochondria membrane permeability and increasing the membrane potential. Furthermore, PAK5 phosphorylates AIF at Thr281 site to inhibit the formation of AIF/importin α3 complex, leading to decrease AIF nuclear translocation. Functionally, we demonstrate that PAK5-mediated AIF phosphorylation promotes the proliferation of breast cancer cells and accelerates the growth of breast cancer in vivo. Significantly, PAK5 and AIF expression in breast cancer are positively correlated with poor patient prognosis. PAK5 expression is negatively correlated with AIF nuclear translocation. These results suggest that PAK5-AIF signaling pathway may play an essential role in mammary tumorigenesis, providing a new therapeutic target for the treatment of breast cancer.

Anisotropic cellulose nanofiber/chitosan aerogel with thermal management and oil absorption properties.

Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications. However, mechanical robustness and functionalization remain huge challenges. Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process. The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times). The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %). The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction). Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents. Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.

Fabrication of cellulose nanocrystal from Carex meyeriana Kunth and its application in the adsorption of methylene blue.

Cellulose nanocrystal (CNC) was extracted from Carex meyeriana Kunth (CMK) by a combination of TEMPO oxidation and mechanical homogenization method, and used to remove methylene blue (MB) from aqueous solution. After alkali-oxygen treatment, the aqueous biphasic system (polyethylene glycol/inorganic salt) was applied to further remove lignin from CMK. The characteriazation of CNC, and the effects of H2O2 dosage, CNC dosage, adsorption time, and initial MB concentration on the MB removal capacity of CNC were investigated. The results showed that the removal percentage of MB by CNC was raised with the increase of H2O2 and CNC dosage. The adsorption kinetics of prepared CNC followed the pseudo-second-order model, and the adsorption isotherms fitted well to the Langmuir model with a calculated maximum adsoption capacity of 217.4mg/g, which was higher than those of CNC extracted by acid hydrolysis method, indicating CNC extracted from CMK had promising potentials in the field of MB adsorption.