ABSTRACT
Rapid slip, at rates in the order of 1 m/s or more, may induce frictional melting in rocks during earthquakes. The short-lived melting has been thought to be a disequilibrium process, for decades. We conducted frictional melting experiments on acidic, basic, and ultrabasic silicate rocks at a slip rate of 1.3 m/s. The experiments and microstructural observations reveal that all minerals in the rocks are melted at temperatures below their known melting temperatures (Tm); e.g., quartz is melted at ~ 1000-1200 °C, not ~ 1720 °C, while olivine at ~ 1300 °C, rather than ~ 1700 °C. The low-temperature melting is incompatible with the conventional disequilibrium melting, and may be caused predominantly by grain size reduction and phase boundary reactions during the early and later stages of slip, respectively. The newly estimated Tm and the melting mechanisms should be considered for understanding the mechanics of earthquakes, landslides, and caldera collapses.
ABSTRACT
A bacterial strain, designated B301T and isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomic approach. Cells were gram-negative, non-motile, obligate-aerobic coccobacilli that were catalase-positive and oxidase-negative. The optimum growth conditions were 30°C, pH 7.0, and 0% NaCl in tryptic soy broth. Colonies were round, convex, smooth, and cream-colored on tryptic soy agar. Strain B301T has a genome size of 3,102,684 bp, with 2,840 protein-coding genes and 102 RNA genes. The 16S rRNA gene analysis revealed that strain B301T belongs to the genus Acinetobacter and shares highest sequence similarity (97.12%) with A. celticus ANC 4603T and A. sichuanensis WCHAc060041T. The average nucleotide identity and digital DNA-DNA hybridization values for closely related species were below the cutoff values for species delineation (95-96% and 70%, respectively). The DNA G+C content of strain B301T was 37.0%. The major respiratory quinone was Q-9, and the cellular fatty acids were primarily summed feature 3 (C16:1 ω6c/C16:1 ω7c), C16:0, and C18:1 ω9c. The major polar lipids were phosphatidylethanolamine, diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidyl-serine. The antimicrobial resistance profile of strain B301T revealed the absence of antibiotic-resistance genes. Susceptibility to a wide range of antimicrobials, including imipenem, minocycline, ampicillin, and tetracycline, was also observed. The results of the phenotypic, chemotaxonomic, and phylogenetic analyses indicate that strain B301T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter pullorum sp. nov. is proposed. The type strain is B301T (=KACC 21653T = JCM 33942T).
Subject(s)
Acinetobacter/classification , Phylogeny , Poultry/microbiology , Acinetobacter/cytology , Acinetobacter/drug effects , Acinetobacter/physiology , Animals , Anti-Bacterial Agents/pharmacology , Base Composition , Chickens , DNA, Bacterial/genetics , Fatty Acids/chemistry , Genes, Bacterial , Genome, Bacterial , Microbial Sensitivity Tests , Nucleic Acid Hybridization , Phospholipids/chemistry , Quinones/chemistry , RNA, Ribosomal, 16S/genetics , Republic of Korea , Sequence Analysis, DNAABSTRACT
A novel bacterial strain, named S23T, was isolated from chicken meat of local market in Korea. Cells were Gram-negative, milky-yellow colored, non-motile and coccobacillus. The strain was obligate aerobic and catalase-positive, oxidase-negative, optimum growth temperature and pH were 25 °C and pH 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain S23T belongs to the genus Acinetobacter and is most closely related to Acinetobacter defluvii KCTC 52503 T (97.40%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) value between strain S23T and its closet phylogenetic neighbors was below 76% and 17%, respectively. The G + C content of genomic DNA of strain S23T was 41.53 mol%. The major respiratory quinone was Q-9. The major cellular fatty acids were summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c), C18:1ω9c, and C16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanol-amine, and phosphatidylserine. The ANI and dDDH results and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain S23T represented a novel species within the genus Acinetobacter, for which the name Acinetobacter pullicarnis sp. nov. is proposed. The strain type is S23T (= KACC 19921 T = JCM 33150 T).
Subject(s)
Acinetobacter/classification , Acinetobacter/genetics , Chickens/microbiology , Meat/microbiology , Acinetobacter/isolation & purification , Animals , Base Composition , DNA, Bacterial/genetics , Fatty Acids/analysis , Nucleic Acid Hybridization , Phylogeny , Quinones/analysis , RNA, Ribosomal, 16S/genetics , Republic of Korea , Sequence Analysis, DNAABSTRACT
A Gram-stain-negative bacterial strain, designated CA10T, was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 °C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain CA10T belonged to the genus Chryseobacterium, family Flavobacteriaceae, and was most closely related to Chryseobacterium indoltheticum ATCC 27950T (98.75â% similarity). The average nucleotide identity and digital DNA-DNA hybridization values of strain CA10T were 94.4 and 56.9â%, respectively, relative to Chryseobacterium scophthalmum DSM 16779T, being lower than the cut-off values of 95-96 and 70â%, respectively. The predominant respiratory quinone was menaquinone-6; major polar lipid, phosphatidylethanolamine; major fatty acids, iso-C15â:â0, summed feature 9 (iso-C17â:â1ω9c and/or C16â:â0 10-methyl), summed feature 3 (iso-C15â:â0 2-OH and/or C16â:â1ω7c) and iso-C17â:â0 3-OH. The results of physiological, chemotaxonomic and biochemical analyses suggested that strain CA10T is a novel species of genus Chryseobacterium, for which the name Chryseobacterium mulctrae sp. nov. is proposed. The type strain is CA10T (=KACC 21234T=JCM 33443T).
Subject(s)
Chryseobacterium/classification , Milk/microbiology , Phylogeny , Animals , Bacterial Typing Techniques , Base Composition , Cattle , Chryseobacterium/isolation & purification , DNA, Bacterial/genetics , Fatty Acids/chemistry , Female , Nucleic Acid Hybridization , Phosphatidylethanolamines/chemistry , RNA, Ribosomal, 16S/genetics , Republic of Korea , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistryABSTRACT
High-velocity weakening of faults may drive fault motion during large earthquakes. Experiments on simulated faults in Carrara marble at slip rates up to 1.3 meters per second demonstrate that thermal decomposition of calcite due to frictional heating induces pronounced fault weakening with steady-state friction coefficients as low as 0.06. Decomposition produces particles of tens of nanometers in size, and the ultralow friction appears to be associated with the flash heating on an ultrafine decomposition product. Thus, thermal decomposition may be an important process for the dynamic weakening of faults.