ABSTRACT
Encephalomyocarditis virus can cause myocarditis and encephalitis in pigs and other mammals, thus posing a potential threat to public health safety. The 2A protein is an important virulence factor of EMCV. Previous studies have shown that the 2A protein may be related to the inhibition of apoptosis by virus, but its specific molecular mechanism is not clear. In this study, the 2A protein was expressed in Escherichia coli in order to find interacting cell proteins. A pull down assay, coupled with mass spectrometry, revealed that the 2A protein possibly interacted with annexin A2. Co-immunoprecipitation assays and confocal imaging analysis further demonstrated that the 2A protein interacted with annexin A2 in cells. In reducing the expression of annexin A2 by siRNA, the ability of the 2A protein to inhibit apoptosis was weakened and the proliferation of EMCV was slowed down. These results suggest that annexin A2 is closely related to the inhibition of apoptosis by 2A. Furthermore, both RT-PCR and western blot results showed that the 2A protein requires annexin A2 interaction to inhibit apoptosis via JNK/c-Jun pathway. Taken together, our data indicate that the 2A protein inhibits apoptosis by interacting with annexin A2 via the JNK/c-Jun pathway. These findings provide insight into the molecular pathogenesis underlying EMCV infection.
Subject(s)
Annexin A2/metabolism , Apoptosis , Encephalomyocarditis virus/physiology , JNK Mitogen-Activated Protein Kinases/metabolism , Proto-Oncogene Proteins c-jun/metabolism , Viral Proteins/metabolism , Animals , Annexin A2/genetics , Apoptosis/genetics , Cell Line , Cricetinae , JNK Mitogen-Activated Protein Kinases/genetics , MAP Kinase Signaling System , Protein Binding , Proto-Oncogene Proteins c-jun/genetics , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Swine , Viral Proteins/genetics , Virus ReplicationABSTRACT
This study was carried out to investigate the biological characteristics of the foot-and-mouth disease (FMD) virus strain Asia-1 China/2005, which is responsible for the 2005 epidemic in China. The result showed that this strain is not host restricted, and could not only cause FMD in cattle and sheep but also in pigs by either inoculation or direct contact.
