ABSTRACT
Elastin-like polypeptides (ELPs) are stimulus-responsive protein-based biopolymers that exhibit phase transition behavior. By joining them to synthetic phytochelatin (EC), EC-ELP fusion proteins with temperature sensitivity and metal-binding functionality were generated to remove heavy metal ions biologically. Three different EC domains (EC10, EC20, EC30) were incorporated into the ELP, and the EC-ELP fusion proteins were expressed in E. coli. Their thermal properties and metal binding abilities were then investigated according to the EC length. In addition, the feasibility of reusing EC-ELPs and the cadmium ion binding affinity of reused EC-ELPs were explored.
Subject(s)
Cadmium/metabolism , Elastin/metabolism , Phytochelatins/metabolism , Cadmium/isolation & purification , Cloning, Molecular , Elastin/chemistry , Elastin/genetics , Escherichia coli/genetics , Gene Expression , Metals, Heavy/isolation & purification , Metals, Heavy/metabolism , Peptides/chemistry , Peptides/genetics , Peptides/metabolism , Phase Transition , Phytochelatins/chemistry , Phytochelatins/genetics , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Temperature , Water Pollutants, Chemical/isolation & purification , Water Pollutants, Chemical/metabolismABSTRACT
The purpose of this study was to determine the effectiveness of antihistamine therapy for withdrawal movements caused by rocuronium injection. One hundred seventy one ASA I-II adults undergoing elective surgery were randomly assigned to one of two groups. Patients in the control group (Group C) were premedicated with 2 mL normal saline, and those in the antihistamine group (Group A) were pre-medicated with 2 mL (45.5 mg) pheniramine maleate. After the administration of thiopental sodium 5 mg/kg, rocuronium 0.6 mg/kg was injected. Withdrawal movements were assessed using a four-grade scale. The administration of antihistamine reveals lower grade of withdrawal movement after rocuronium injection.
