Sequentially suspended 3D bioprinting of multiple-layered vascular models with tunable geometries forin vitromodeling of arterial disorders initiation.

As the main precursor of arterial disorders, endothelial dysfunction preferentially occurs in regions of arteries prone to generating turbulent flow, particularly in branched regions of vasculatures. Although various diseased models have been engineered to investigate arterial pathology, producing a multiple-layered vascular model with branched geometries that can recapitulate the critical physiological environments of human arteries, such as intercellular communications and local turbulent flows, remains challenging. This study develops a sequentially suspended three-dimensional bioprinting (SSB) strategy and a visible-light-curable decellularized extracellular matrix bioink (abbreviated as 'VCD bioink') to construct a biomimetic human arterial model with tunable geometries. The engineered multiple-layered arterial models with compartmentalized vascular cells can exhibit physiological functionality and pathological performance under defined physiological flows specified by computational fluid dynamics simulation. Using different configurations of the vascular models, we investigated the independent and synergetic effects of cellular crosstalk and abnormal hemodynamics on the initiation of endothelial dysfunction, a hallmark event of arterial disorder. The results suggest that the arterial model constructed using the SSB strategy and VCD bioinks has promise in establishing diagnostic/analytic platforms for understanding the pathophysiology of human arterial disorders and relevant abnormalities, such as atherosclerosis, aneurysms, and ischemic diseases.

Organic fertilization integrated with water management to remediate As and Cd contamination in a paddy soil.

Soil heavy metal pollution is the main risk for sustainable agriculture, especially the combination of As and Cd pollution in paddy fields which may lead to the superimposed accumulation in rice. There is an urgent need for environmental-friendly and cost-effective strategies to remediate the contamination of As and Cd in soils. In this work, a pot culture experiment was conducted in a As and Cd polluted paddy soil to explore the effects of organic fertilization (OF) and two water managements (continuous flooding, CF; intermittent irrigation, II) on the fractionation of As and Cd in soil, and the uptake of As and Cd by rice. The results showed that OF integrated with intermittent irrigation performed best in reducing the contents of As and Cd in rice grains by 58.9 % and 69.3 %, respectively, under compound pollution. The significant conversion of available As and Cd to stable species (specifically adsorbed and Fe-Mn/Al oxide bound) under OF + II were supported by the changes in an array of soil attributes such as pH, Eh, soluble Fe and dissolved organic carbon (DOC). Intermittent irrigation was more conducive to the accumulation of As outside the roots, and Fe-plaque prevented As uptake by roots and the translocation to shoots. While more accumulation of Fe-plaque along with Cd on root surface induced by continuous flooding is helpful for depressed assimilation of Cd by rice. Considering the combined contamination of As and Cd polluted in paddy soils, a management approach was proposed based on intermittent irrigation and application of organic fertilizer at the rate of 0.1 % (∼ 2.3 t/ha) in two phases (two weeks before planting or drainage). Organic fertilization will hold great promise in restoring polluted soils and maintaining soil health via suppressing the lability of heavy metals and providing nutrients.

Elevated temperature induces contrasting transformation of exogenous copper to soil solution and solid phases in an arable soil.

Heavy metal contamination of soils has been a global environmental issue over the past decades, threatening food security and human health. Understanding the migration and transformation of heavy metals in soils is critical for restoring an impaired environment and developing sustainable agriculture, particularly in the face of global warming. However, little effort has been devoted to investigating the impact of elevated temperatures on the migration and distribution of exogenous heavy metals in soils. This study experimented with a 180-day incubation at 15 °C, 30 °C, and 45 °C with an arable soil (Alfisol) of Huang-Huai-Hai River Basin, China, which was initially spiked with copper (Cu). A comparison of the results revealed that the percentage of soil water-soluble Cu doubled at 45 °C compared with 15 °C. The percentage of protein-like substances in dissolved organic matter (DOM) was the highest at 45 °C, suggesting that proteinaceous components play a more significant role in controlling the dissolution of Cu into DOM. Moreover, by sequential extraction and micro-X-ray fluorescence (µ-XRF), Cu was facilitatively transformed from exchangeable, and specifically adsorbed fractions, to iron (Fe)/manganese (Mn) oxides bound species by 7.75%23.63% with the elevation of temperature from 15 °C to 45 °C. The conversion of Cu speciation is attributed to the significant release of organic carbon from Fe/Mn oxides, especially the Mn oxide components, which are available for Cu binding. The findings of this work will provide an in-depth understanding of the fate of Cu in soils, which is fundamental for the risk assessment and remediation of Cu-polluted soils in the Huang-Huai-Hai River Basin under the context of global warming.

Remaining Useful Life Prediction for Two-Phase Nonlinear Degrading Systems with Three-Source Variability.

Recently, the estimation of remaining useful life (RUL) for two-phase nonlinear degrading devices has shown rising momentum for ensuring their safe and reliable operation. The degradation processes of such systems are influenced by the temporal variability, unit-to-unit variability, and measurement variability jointly. However, current studies only consider these three sources of variability partially. To this end, this paper presents a two-phase nonlinear degradation model with three-source variability based on the nonlinear Wiener process. Then, the approximate analytical solution of the RUL with three-source variability is derived under the concept of the first passage time (FPT). For better implementation, the offline model parameter estimation is conducted by the maximum likelihood estimation (MLE), and the Bayesian rule in conjunction with the Kalman filtering (KF) algorithm are utilized for the online model updating. Finally, the effectiveness of the proposed approach is validated through a numerical example and a practical case study of the capacitor degradation data. The results show that it is necessary to incorporate three-source variability simultaneously into the RUL prediction of the two-phase nonlinear degrading systems.

A Novel Unified Data Modeling Method for Equipment Lifecycle Integrated Logistics Support.

Integrated logistics support (ILS) is of great significance for maintaining equipment operational capability in the whole lifecycle. Numerous segments and complex product objects exist in the process of equipment ILS, which gives ILS data multi-source, heterogeneous, and multidimensional characteristics. The present ILS data cannot satisfy the demand for efficient utilization. Therefore, the unified modeling of ILS data is extremely urgent and significant. In this paper, a unified data modeling method is proposed to solve the consistent and comprehensive expression problem of ILS data. Firstly, a four-tier unified data modeling framework is constructed based on the analysis of ILS data characteristics. Secondly, the Core unified data model, Domain unified data model, and Instantiated unified data model are built successively. Then, the expressions of ILS data in the three dimensions of time, product, and activity are analyzed. Thirdly, the Lifecycle ILS unified data model is constructed, and the multidimensional information retrieval methods are discussed. Based on these, different systems in the equipment ILS process can share a set of data models and provide ILS designers with relevant data through different views. Finally, the practical ILS data models are constructed based on the developed unified data modeling software prototype, which verifies the feasibility of the proposed method.

Investigation of braided stents in curved vessels in terms of "Dogbone" deformation.

"Dogbone" deformation that the diameters of two ends are larger than the middle diameter of the stent under the effect of the balloon expanding, is one of the important standards to evaluate the mechanical properties of vascular stents. It is a huge challenge to simulate and evaluate the "Dogbone" behaviors of braided stents in the curved vessels. In this study, the key work was to investigate the "Dogbone" deformations of braided stents in the curved vessels by designing main parameters including strut diameter, braiding angle, and the circumferential number of unit cell. Based on the "Dogbone" stents in the curved vessels, the impact of "Dogbone" on the fatigue properties of braided stents was analyzed under the pulsatile effect of vessels. The influence of "Dogbone" stents on stress distribution of vascular walls was studied. To evaluate the "Dogbone" behaviors of stents in the curved vessels, the calculation method of "Dogbone" was improved by calculating the centerline and the bus bar of the curved vessels. Braided stents with various parameters (strut diameter t = 100,125 and 152 µm, braiding angle α = 30, 40 and 50°, the circumferential number of unit cell N = 8, 10, and 12) were designed respectively. Numerical simulation method was used to mimic the "Dogbone" deformation after stent expansion. The results showed that strut diameter and braiding angle had more influence on "Dogbone" deformations than the circumferential number of unit cell. "Dogbone" deformation could adversely affect fatigue performance and vascular walls.

Multi-Stream Convolutional Neural Networks for Rotating Machinery Fault Diagnosis under Noise and Trend Items.

In recent years, rotating machinery fault diagnosis methods based on convolutional neural network have achieved much success. However, in real industrial environments, interfering signals are unavoidable, which may reduce the accuracy of fault diagnosis seriously. Most of the current fault diagnosis methods are of single input type, which may lead to the information contained in the vibration signal not being fully utilized. In this study, theoretical analysis and comprehensive comparative experiments are completed to investigate the time domain input, frequency domain input, and two types of time-frequency domain input. Based on this, a new fault diagnosis model, named multi-stream convolutional neural network, is developed. The model takes the time domain, frequency domain, and time-frequency domain images as input, and it automatically fuses the information contained in different inputs. The proposed model is tested based on three public datasets. The experimental results suggested that the model achieved pretty high accuracy under noise and trend items without the help of signal separation algorithms. In addition, the positive implications of multiple inputs and information fusion are analyzed through the visualization of learned features.

The Biofabrication of Diseased Artery In Vitro Models.

As the leading causes of global death, cardiovascular diseases are generally initiated by artery-related disorders such as atherosclerosis, thrombosis, and aneurysm. Although clinical treatments have been developed to rescue patients suffering from artery-related disorders, the underlying pathologies of these arterial abnormalities are not fully understood. Biofabrication techniques pave the way to constructing diseased artery in vitro models using human vascular cells, biomaterials, and biomolecules, which are capable of recapitulating arterial pathophysiology with superior performance compared with conventional planar cell culture and experimental animal models. This review discusses the critical elements in the arterial microenvironment which are important considerations for recreating biomimetic human arteries with the desired disorders in vitro. Afterward, conventionally biofabricated platforms for the investigation of arterial diseases are summarized, along with their merits and shortcomings, followed by a comprehensive review of advanced biofabrication techniques and the progress of their applications in establishing diseased artery models.

Realization of Super-Large-Diameter Slurry Shield Passing through Settlement-Sensitive Area Based on Unreinforced Disturbance Control Technology.

Due to the complex construction conditions of shield tunnels, ground disturbance is inevitable during the construction process, which leads to surface settlement and, in serious cases, damage to surrounding buildings (structures). Therefore, it is especially important to effectively control the constructive settlement of subway tunnels when crossing settlement-sensitive areas such as high-density shantytowns. Based on the project of Wuhan Metro Line 8 Phase I, the shield of Huangpu Road Station-Xujiapang Road Station interval crossing high-density shantytowns, we study the disturbance control technology of oversized diameter mud and water shield crossing unreinforced settlement-sensitive areas during the construction process. By optimizing the excavation parameters and evaluating the ground buildings, the excavation process can be monitored at the same time, and the water pressure, speed, and tool torque required during the excavation during the construction process can be finely adjusted; the control of tunneling process parameters can provide reference and basis for analyzing the construction control of large-diameter shield through old shantytowns.

Elevated temperature altered the binding sequence of Cd with DOM in arable soils.

Dissolved organic matter (DOM) is one of the most active soil components, which plays pivotal roles in the migration and fate of heavy metals in soils. The interactions of heavy metals with DOM are controlled by the structure and properties of DOM. The changes of temperature have a significant effect on the content and composition of DOM and thus may affect the binding nature of heavy metals with DOM. In the current study, we conducted a 180-d incubation experiment with an arable soil at temperatures of 15, 30 and 45 °C. Fluorescence spectroscopy was used to examine the composition of DOM and two-dimensional correlation spectroscopy was applied to determine the binding intensity and sequence between cadmium (Cd) with DOM. Two humic-like substances (C1, C3) and a protein-like substance (C2) were identified from soil DOM. Elevated temperature changed the characteristic and structure of DOM. The humification degree and aromaticity of DOM increased from 15 °C to 30 °C but decreased at high temperature (45 °C). The alterations in temperature exert no impact on the type of organic functional groups in DOM binding with Cd. However, elevated temperature changed the binding sequence of Cd with DOM fractions. Polysaccharide, phenolic, and aromatic groups exhibited the fastest response to Cd at 15, 30, and 45 °C, respectively. These observations would provide a better understanding on the environmental behavior of Cd in arable soils under the context of global warming.

Warming and humidification mediated changes of DOM composition in an Alfisol.

Dissolved organic matter (DOM) represents the most mobile and reactive pool of soil organic matter (SOM). Climate changes, such as global warming and altered precipitation exert considerable influences on the quality and quantity of soil DOM. However, rare reports have focused on the interactive effects of soil warming and increased precipitation. In the present study, we conducted a 90-day incubation experiment to investigate how the concentration, source and chemical composition of DOM from an Alfisol respond to the variations of temperatures (15, 30 and 45 °C) and moistures (40%, 60%, and 80% of saturated soil water content). Four DOM components were identified through fluorescence excitation emission matrix (EEM)-parallel factor analysis (PARAFAC). Increased temperature alone aggravated the decomposition of plant-derived aromatic components (C2 and C4) but promoted the accumulation of microbial-derived aliphatic carbon (C1) and tryptophan-like component (C3). Increased fungi/bacteria ratio with warming was responsible for the decomposition of plant-derived components. Warming-induced disassociation of Ca-bearing mineral to colloidal Ca facilitated the accrual of microbial-derived aliphatic DOM. Humidification alone and humidification + warming significantly increased the concentration of DOM and the percentage of plant-derived aromatic carbon (C2, C4), which was attributed to the release of Fe-bearing mineral-OC. Based on the above findings along with the results of two-way ANOVA and Variation partition analysis, we infer that moisture will play a dominant role in regulating the chemical composition of DOM in Alfisols under both warming and humidification which in turn impact global C cycling and the ultimate climate.

Design of a metamaterial film with excellent conformability and adhesion for bandage substrates.

Developing films with excellent conformability and adhesion has become a research hotspot in many fields, such as medical bandages. The conventional method for enhancing films conformability and adhesion is to make the films thinner or modify the material of the films, which usually compromises the function of the films. In this paper, a novel metamaterial film was proposed to cover the skin area of a human elbow during the rotation of elbow. This structure is composed of unit cells with rectangular perforations, whose Poisson's ratio (PR) is determined by the length of the perforation. With finite element analysis (FEA), relations among the stretch strain, Poisson's ratio and length of the perforation of unit cell was obtained. Then, the proposed film was generated by mapping unit cells with different PR to the target skin surface. With the same deformation behavior as the elbow skin, conformability and adhesion of the generated film can be guaranteed during the entire rotation process of the elbow, which has been verified by both FEA and experimental tests. Theoretically, by changing the arrangement of different PR unit cells, the proposed method can be applied to design films for other complex surface on human body. It also provides a new way to introduce materials with better biocompatibility but poor mechanical properties as bandage substrates. As a possible application, a prototype of smart bandage was developed by installing a high-resolution temperature sensor on the proposed film, which can monitor the inflammation of the wounded skin in real time.

Design and Mechanical Properties Verification of Gradient Voronoi Scaffold for Bone Tissue Engineering.

In order to obtain scaffold that can meet the therapeutic effect, researchers have carried out research on irregular porous structures. However, there are deficiencies in the design method of accurately controlling the apparent elastic modulus of the structure at present. Natural bone has a gradient porous structure. However, there are few studies on the mechanical property advantages of gradient bionic bone scaffold. In this paper, an improved method based on Voronoi-tessellation is proposed. The method can get controllable gradient scaffolds to fit the modulus of natural bone, and accurately control the apparent elastic modulus of porous structure, which is conducive to improving the stress shielding. To verify the designed structure can be fabricated by additive manufacturing, several designed models are obtained by SLM and EBM. Through finite element analysis (FEA), it is verified that the irregular porous structure based on Voronoi-tessellation is more stable than the traditional regular porous structure of the same structure volume, the same pore number and the same material. Furthermore, it is verified that the gradient irregular structure has a better stability than the non-gradient structure. An experiment is conducted successfully to verify the stability performance got by FEA. In addition, a dynamic impact FEA is also performed to simulate impact resistance. The result shows that the impact resistance of the regular porous structure, the irregular porous structure and the gradient irregular porous structure becomes better in turn. The mechanical property verification provides a theoretical basis for the structural design of gradient irregular porous bone tissue engineering scaffolds.

Structural Design of Vascular Stents: A Review.

Percutaneous Coronary Intervention (PCI) is currently the most conventional and effective method for clinically treating cardiovascular diseases such as atherosclerosis. Stent implantation, as one of the ways of PCI in the treatment of coronary artery diseases, has become a hot spot in scientific research with more and more patients suffering from cardiovascular diseases. However, vascular stent implanted into vessels of patients often causes complications such as In-Stent Restenosis (ISR). The vascular stent is one of the sophisticated medical devices, a reasonable structure of stent can effectively reduce the complications. In this paper, we introduce the evolution, performance evaluation standards, delivery and deployment, and manufacturing methods of vascular stents. Based on a large number of literature pieces, this paper focuses on designing structures of vascular stents in terms of "bridge (or link)" type, representative volume unit (RVE)/representative unit cell (RUC), and patient-specific stent. Finally, this paper gives an outlook on the future development of designing vascular stents.

Fabricating Pyramidal Lattice Structures of 304 L Stainless Steel by Wire Arc Additive Manufacturing.

Lattice structures have drawn considerable attention due to their superior mechanical properties. However, the existing fabrication methods for lattice structures require complex procedures, as they have low material utilization and lead to unreliable node connections, which greatly restricts their application. In this work, wire arc additive manufacturing is used to fabricate large-scale lattice structures efficiently, without any air holes between rods and panels. The principle and the process of fabricating the rods were analyzed systematically. The influence of the two most important parameters, including heat input and preset layer height, is disclosed. Through optical microscopy, the microstructure of the fabricated steel rods is found to consist of dendritic austenite and skeletal ferrite. The tensile strength of the rods can reach 603 MPa, and their elongation reaches 77%. These experimental results demonstrated the feasibility of fabricating lattice structures using wire arc additive manufacturing.

Experimental study of high-flow and low-expansion backfill material.

High-flow low-expansion backfill materials have been developed to improve difficult slurry pipeline transport and poor roof-contact effect of many filling materials. The fly ash content was fixed at 80%, with 8.5% - 9.5% mineral powder content, 8.5% - 9.5% lime, 2% - 3% desulfurized gypsum, 0.9% - 1.2% sodium carbonate, and 0.01% - 0.02% aluminum powder content. The prepared backfill material processed good fluidity, with the expansion rate of the hardened material reaching 2% - 3%, and compressive strength on 90 d reaching 4 MPa-5.5 MPa. SEM observations indicated that as the aluminum content increased, ettringite on bubble walls transformed from a fine-needle to needle-rod shape. Secondly, the hydration products of the system were mainly hydrated calcium silicate gel and ettringite, which interconnected and promoted the formation of the structure. The backfill material has extensive sources of raw materials, low cost, simple filling process, and good filling effect.

Resveratrol alleviate the injury of mice liver induced by cadmium sulfide nanoparticles.

Cadmium sulfide nanoparticle (Nano-CdS) is a kind of important semiconductor material with special photochemistry property. With the Nano-CdS being widely used, the security problems it caused have been catching more and more attention. This study aims to explore the possible mechanism of liver injury induced by Nano-CdS and whether resveratrol can reduce the damage. In this study, male BALB/C mice were treated with Nano-CdS with a diameter of 20 to 30 nm and a length of 80 to 100 nm. It turned out that the mice liver inflammatory cells infiltrated, the liver tissue and the ultrastructure changed; The activities of T-AOC and GSH were suppressed (n = 6, P < 0.05) and the content of lipid peroxide (MDA) increased (n = 6, P < 0.05). Besides, Nano-CdS decreased the mRNA expression level of Sirt1 and FoxO1 genes in liver tissue (n = 3, P < 0.05). All the changes in the index were reversed by resveratrol. The mRNA expression level of FoxO3a showed no significant difference between the control group and the Nano-CdS group. But under the protection of resveratrol, the mRNA expression level of FoxO3a was higher than that in the control and Nano-CdS groups (n = 3, P < 0.05). Results suggest that Nano-CdS can cause oxidative damages to liver tissues in mice, in which process that the Sirt1 and FoxO1 genes may participate, and the damage can be reversed by resveratrol which may be a potential cure for oxidative damage to nanomaterials.

Optimizing the deformation behavior of stent with nonuniform Poisson's ratio distribution for curved artery.

Stent implantation at a highly curved artery has always been a challenge, considering the relatively high chance of in-stent restenosis (ISR) caused by severe straightening effect and high strain energy over the vessel wall. In this paper, a novel optimization based design method was proposed to manipulate the deformation behavior of the common ring-and-link stent. By changing the location of the connection point between rings and links, traditional ring-and-link structure was modified to achiever tunable Poisson's ratio (PR). With the nonuniform cellular structure design method proposed in a previous study, PR distribution of the stent structure was optimized to achieve the desired curvature. As a result, the obtained stent structure with nonuniform PR could perfectly fit into the curved artery after expansion, without causing any obvious vessel straightening. To validate the proposed method, two different vessel models were introduced. Firstly, a short vessel with a constant curvature was set as the design objective, and both numerical and experimental tests were conducted. Further, a patient-specific vessel was applied. Both test results showed that optimized stents would cause much smaller vessel straightening. Moreover, vessels stented by the optimized structures had much lower stress concentration and strain energy. All those properties will decrease the possibility of ISR significantly.