ABSTRACT
A Gram-stain-negative, non-motile, facultatively anaerobic and non-flagellated marine bacterium, designated JC070T was isolated from the Yongle Blue Hole in the South China Sea. The temperature, pH and NaCl ranges for growth of strain JC070T were 4-37 °C (optimum, 16 °C), pH 6.0-9.0 (optimum, pH 7.0) and 1.0 -6.0% (w/v; optimum, 3.0%). The predominant isoprenoid quinone of strain JC070T was identified as menaquinone-7. The dominant fatty acids (>10%) were iso-C15:0 (59.6%) and iso-C17:0 3-OH (17.2%). The major polar lipids were aminophospholipid, aminolipid, two unknown phospholipids and two unidentified lipids. The genomic DNA G+C content was determined to be 37.0 mol%. Based on the results of polyphasic analysis, a new species, named Marinifilum caeruleilacunae sp. nov., within the genus Marinifilum was proposed. The type strain is JC070T (= JCM 39045T=MCCC 1K03774T).
Subject(s)
Flavobacteriaceae , Bacterial Typing Techniques , Bacteroidetes , Base Composition , China , DNA, Bacterial/genetics , Fatty Acids/chemistry , Phylogeny , RNA, Ribosomal, 16S/genetics , Seawater/microbiology , Sequence Analysis, DNAABSTRACT
A Gram-stain-negative, obligately anaerobic, non-motile, non-spore-forming, long-rod-shaped and non-flagellated bacterial strain, designated T3-2 S1-CT, was isolated from a sediment sample collected at the Okinawa Trough. Phylogenetic analyses of 16S rRNA gene sequences and the whole genome revealed that strain T3-2 S1-CT was a member of the family Marinifilaceae and exhibited less than 95.1 % sequence similarities to the closely related type strains of the family Marinifilaceae. Optimal growth occurred at pH 7.0, 28 °C and in the presence of 3 % (w/v) NaCl. The isoprenoid quinone of strain T3-2 S1-CT was identified as menaquinone-7 (MK-7) and the predominant fatty acids (>10 %) were iso-C15 : 0 (38.9 %) and anteiso-C15 : 0 (11.6 %). The major polar lipids were one phosphatidylethanolamine, one phosphatidylmonomethylethanolamine, one aminolipids, two unidentified lipids and two unidentified phospholipids. The DNA G+C content of strain T3-2 S1-CT was 35.7 mol%. On the basis of the results of polyphasic analyses, strain T3-2 S1-CT is considered to represent a novel species of the genus Ancylomarina, for which the name Ancylomarina longa sp. nov. is proposed. The type strain is T3-2 S1-CT (=KCTC 15505T=MCCC 1K01617T).
Subject(s)
Bacteroidetes/classification , Phylogeny , Bacterial Typing Techniques , Bacteroidetes/isolation & purification , Base Composition , DNA, Bacterial/genetics , Fatty Acids/chemistry , Pacific Ocean , Phospholipids/chemistry , RNA, Ribosomal, 16S/genetics , Seawater/microbiology , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistryABSTRACT
A Gram-stain negative, obligately anaerobic, non-motile, asporogenous long rod-shaped and non-flagellated bacterial strain, designated 4SWWS2-6T, was isolated from sediment in the intertidal zone of Fildes Peninsula, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4SWWS2-6T belongs to the genus Ancylomarina and showed high sequence similarity with Ancylomarina subtilis FA102T (96.5%). Optimal growth occurred at pH 6.5, 16 °C and in the presence of 3% (w/v) NaCl. Strain 4SWWS2-6T contained menaquinone-7 (MK-7) as the major respiratory quinone and held iso-C15:0, anteiso-C15:0 and iso-C15:0 3-OH as the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an aminolipid, two unidentified lipids and an unidentified phospholipid. The DNA G + C content of strain 4SWWS2-6T was 37.6 mol%. On the basis of the polyphasic analyses, strain 4SWWS2-6T is considered to represent a novel species in the genus Ancylomarina, for which the name Ancylomarina psychrotolerans sp. nov. is proposed. The type strain is 4SWWS2-6T (= KCTC 15504T = MCCC 1K01618T).
Subject(s)
Bacteroidetes/classification , Bacteroidetes/isolation & purification , Geologic Sediments/microbiology , Antarctic Regions , Bacterial Typing Techniques , Bacteroidetes/genetics , Bacteroidetes/metabolism , DNA, Bacterial/genetics , Fatty Acids/chemistry , Fatty Acids/metabolism , Phospholipids/metabolism , Phylogeny , RNA, Ribosomal, 16S/geneticsABSTRACT
A Gram-stain-negative, non-endospore-forming, strictly aerobic, irregular rod-shaped bacterium without flagellum, designated strain H94T, was isolated by the high-throughput cultivation method from seawater of an amphioxus breeding zone in the coastal region of Qingdao, China. Growth was observed at 4-37 °C (optimum 28 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 1-12 % (w/v) NaCl (optimum 1-2 %). The predominant cellular fatty acids were C18 : 1ω9c, C16 : 0 and C16 : 1ω9c. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol and an unidentified phosphoglycolipid. The major respiratory quinone was ubiquinone-9 (Q-9). The genomic DNA G+C content of strain H94T was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H94T shared the highest similarity (95.9 %) with Hahella antarctica NBRC 102683T, and exhibited 92.9 % and 92.1 % similarity with the two other recognized Hahella species, Hahella chejuensis KCTC 2396T and Hahella ganghwensis DSM 17046T, respectively. The phylogenetic position revealed that strain H94T formed a stable distinct lineage cluster together with Hahella antarctica NBRC 102683T and this result was further confirmed by multilocus sequence analysis based on housekeeping genes gyrB and rpoB. On the basis of the polyphasic taxonomic analyses, strain H94T is considered to represent a novel species in a new genus, for which the name Allohahellamarinimesophila gen. nov., sp. nov. is proposed. The type strain of Allohahellamarinimesophila is H94T (=CGMCC 1.10800T=JCM 17555T). It is also proposed that Hahella antarctica should be reclassified within the genus Allohahella as Allohahella antarctica comb. nov. (type strain NBRC 102683T=IMCC 3113T=KCCM 42675T). The type species of the genus Allohahella is Allohahella antarctica comb. nov.
