ABSTRACT
Metallized arrays of three-dimensional (3D) nanoarchitectures offer new and exciting prospects in nanophotonics and nanoelectronics. Engineering these repeating nanoarchitectures, which have dimensions smaller than the wavelength of the light source, enables in-depth investigation of unprecedented light-matter interactions. Conventional metal nanomanufacturing relies largely on lithographic methods that are limited regarding the choice of materials and machine write time and are restricted to flat patterns and rigid structures. Herein, we present a 3D nanoprinter devised to fabricate flexible arrays of 3D metallic nanoarchitectures over areas up to 4 × 4 mm2 within 20 min. By suitably adjusting the electric and flow fields, metal lines as narrow as 14 nm were printed. We also demonstrate the key ability to print a wide variety of materials ranging from single metals, alloys to multimaterials. In addition, the optical properties of the as-printed 3D nanoarchitectures can be tailored by varying the material, geometry, feature size, and periodic arrangement. The custom-designed and custom-built 3D nanoprinter not only combines metal 3D printing with nanoscale precision but also decouples the materials from the printing process, thereby yielding opportunities to advance future nanophotonics and semiconductor devices.
ABSTRACT
The aim of this study was to explore the primary chemical properties and anti-fatigue effect in vivo of Pholiota nameko polysaccharide (PNP). Through UV-visible spectrum, the absorption peaks of proteins, nucleic acids and pigments were not found. The organic functional groups of polysaccharides (3,289.97, 1,584.72, and 1,045.23 cm-1 so on) were measured by IR spectroscopy. The PNP was a semi-crystalline or non-crystalline substance, possessed a three-dimensional lump structure with a smooth, dense surface and amorphous structure according to the scanning electron microscopy and XRD images. Moreover, the PNP was chain or bright-spot structures formed by the entanglement of multiple polysaccharide fibers on the basis of atomic force microscopy. The results of anti-fatigue suggested the PNP could significantly extend the forced swim time from 121.58 ± 18.48 and 101.91 ± 14.27 min to 154.95 ± 24.26 and 134.13 ± 25.71 min in male and female mice respectively. The LDH activity was up to 31.68 ± 4.60 U/ml in male mice and 29.49 ± 5.12 U/ml in female mice. Meanwhile, the Ca2+ -Mg2+ -ATPase activity was reached to 2.49 ± 0.41 µmol/(mg·h) in male mice and 2.44 ± 0.29 µmol/(mg·h) in female mice. The SOD activity was increased to 5.92 ± 1.19 U/ml in male mice and 5.89 ± 0.98 U/ml in female mice, while the MDA content was decreased to 2.24 ± 0.34 nmol/mg in male mice and 2.02 ± 0.41 nmol/mg in female mice. These results showed a theoretical basis for application of the PNP in food and pharmacy as a natural physical strengthening substance. PRACTICAL APPLICATIONS: Fatigue affects physical and mental health in vivo, which resulted in negative effects on everyday tasks, leisure activities, cognitive and behavioral performances and is very common in modern life. Therefore, this study was designed to explore the primary chemical properties and research the anti-fatigue effects of Pholiota nameko polysaccharide (PNP) in mice. And then, it would be a reference for the development and utilization of PNP as a kind of healthy food on sub-health.